LOCUS       sequence-305589-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of DYRK4 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-DYRK4_IDG-K
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..136
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgataaacag ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg
       61 agaaatcagt ctgcggtctg ccgaaaaggc tagcggcaag cccatcccca accccctgct
      121 gggcctggac agcaccacaa gtttgtacaa aaaagcaggc tccaccatgc cggcctcaga
      181 gctcaaggct tcagaaatac ctttccaccc tagcattaaa acccaggatc ccaaggcaga
      241 ggagaagtca ccaaagaagc aaaaggtgac tctgacagcg gcagaggccc taaagctttt
      301 taagaaccag ctgtctccat atgaacaaag tgaaatcctg ggctacgcgg agctgtggtt
      361 cctgggtctt gaagccaaga agctcgacac ggctcctgag aaatttagca agacgagttt
      421 tgatgatgag catggcttct atctgaaggt tctgcatgat cacattgcct accgctatga
      481 agttctggag acaatcggga aggggtcctt tggacaggtg gccaagtgct tggatcacaa
      541 aaacaatgag ctggtggccc tgaaaatcat caggaacaag aagaggtttc accagcaggc
      601 cctgatggag ctgaagatcc tggaagctct cagaaagaag gacaaagaca acacctacaa
      661 tgtggtgcat atgaaggact ttttctactt tcgcaatcac ttctgcatca cctttgagct
      721 cctgggaatc aacttgtatg agttgatgaa gaataacaac tttcaaggct tcagtctgtc
      781 catagttcgg cgcttcactc tctctgtttt gaagtgcttg cagatgcttt cggtagagaa
      841 aatcattcac tgtgatctca agcccgaaaa tatagtgcta taccaaa
//
