LOCUS       sequence-305596-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of PHKG2 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-PHKG2_IDG-K
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..136
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgataaacag ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg
       61 agaaatcagt ctgcggtctg ccgaaaaggc tagcggcaag cccatcccca accccctgct
      121 gggcctggac agcaccacaa gtttgtacaa aaaagcaggc tccaccatga cgctggacgt
      181 ggggccggag gatgagctgc ccgactgggc cgccgccaaa gagttttacc agaagtacga
      241 ccctaaggac gtcatcggca gaggagtgag ctctgtggtc cgccgttgtg ttcatcgagc
      301 tactggccac gagtttgcgg tgaagattat ggaagtgaca gctgagcggc tgagtcctga
      361 gcagctggag gaggtgcggg aagccacacg gcgagagaca cacatccttc gccaggtcgc
      421 cggccacccc cacatcatca ccctcatcga ttcctacgag tcttctagct tcatgttcct
      481 ggtgtttgac ctgatgcgga agggagagct gtttgactat ctcacagaga aggtggccct
      541 ctctgaaaag gaaaccaggt ccatcatgcg gtctctgctg gaagcagtga gctttctcca
      601 tgccaacaac attgtgcatc gagatctgaa gcccgagaat attctcctag atgacaatat
      661 gcagatccga ctttcagatt tcgggttctc ctgccacttg gaacctggcg agaagcttcg
      721 agagttgtgt gggaccccag ggtatctagc gccagagatc cttaaatgct ccatggatga
      781 aacccaccca ggctatggca aggaggtcga cctctgggcc tgtggggtga tcttgttcac
      841 actcctgg
//
