LOCUS       sequence-305599-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of ICK (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-ICK_IDG-K
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(114..134)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      135..144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga
       61 ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac ggccaacttt
      121 tgtatacaaa gtgggccacc atgggaatcc cgctgctgaa cgctaaacag attctgggac
      181 agctggacgg cgggagcgtg gcagtcctgc ctgtggtcga ctccaccaat cagtacctgc
      241 tggatcgaat cggcgagctg aagagtgggg atgcttgcat tgcagaatat cagcaggcag
      301 ggagaggaag cagagggagg aaatggttct ctccttttgg agctaacctg tacctgagta
      361 tgttttggcg cctgaagcgg ggaccagcag caatcggcct gggcccggtc atcggaattg
      421 tcatggcaga agcgctgcga aagctgggag cagacaaggt gcgagtcaaa tggcccaatg
      481 acctgtatct gcaggataga aagctggcag gcatcctggt ggagctggcc ggaataacag
      541 gcgatgctgc acagatcgtc attggcgccg ggattaacgt ggctatgagg cgcgtggagg
      601 aaagcgtggt caatcagggc tggatcacac tgcaggaagc agggattaac ctggacagga
      661 atactctggc cgctatgctg atccgagagc tgcgggcagc cctggaactg ttcgagcagg
      721 aaggcctggc tccatatctg tcacggtggg agaagctgga taacttcatc aatagacccg
      781 tgaagctgat cattggggac aaagagattt tcgggattag ccggggga
//
