LOCUS       sequence-305600-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of ICK (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-ICK_IDG-K
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..136
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgataaacag ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg
       61 agaaatcagt ctgcggtctg ccgaaaaggc tagcggcaag cccatcccca accccctgct
      121 gggcctggac agcaccacaa gtttgtacaa aaaagcaggc tccaccatga atagatacac
      181 aacaatcagg cagctcgggg atggaaccta cggttccgtc ctgctgggaa gaagcattga
      241 gtctggggag ctgatcgcta ttaaaaaaat gaaaagaaaa ttttattcct gggaggaatg
      301 catgaacctt cgggaggtta agtctttaaa gaagctcaac catgccaatg tagtcaaatt
      361 aaaagaagtt atcagggaaa atgatcatct ttattttatc ttcgagtaca tgaaggaaaa
      421 tctttaccag ctcattaaag agagaaataa gttgtttcct gagtctgcta taaggaatat
      481 catgtatcag atattacaag gactcgcatt tattcacaaa cacggcttct ttcatcgaga
      541 cttaaagcct gagaacctcc tctgcatggg accagaactt gtgaaaattg cagactttgg
      601 tttggcccga gaaatacgat caaaacctcc atatacagat tatgtatcta ccagatggta
      661 cagggctcca gaagtactcc tgaggtctac caactacagc tcccccattg acgtctgggc
      721 ggtgggctgc atcatggcag aagtttacac cctcaggcca ctcttccctg gagccagtga
      781 aattgacaca atattcaaaa tttgccaagt gctggggaca ccaaaaaaga ctgactggcc
      841 tg
//
