LOCUS       sequence-30562-e         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 T7 DLC1
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             86..118
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(452..470)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(453..470)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(490..507)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    496..607
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agcagagctc tctggctact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcccctttn gggagaccca agcttatggc tagcatgact ggtggacagc aaatgggtgg
      121 atcctgcgac cgaaaggccg tgatcaaaaa tgcggacatg tcggaagaga tgcaacagga
      181 ctcggtggag tgcgctactc aggcgctgga gaaatacaac atagagaagg acattgcggc
      241 tcatatcaag aaggaatttg acaagaagta caatcccacc tggcattgca tcgtggggag
      301 gaacttcggt agttatgtga cacatgaaac caaacacttc atctacttct acctgggcca
      361 agtggccatt cttctgttca aatctggtta agaattctgc agatatccat cacactggcg
      421 gccgctcgag catgcatcta gagggcccta ttctatagtg tcacctaaat gctagagctc
      481 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
      541 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
      601 ttgcatcgca ttgtctgagt angtgtcatt ctattctggg gggtggggtg gggcaggaca
      661 gcaaggggga ggatgggaan acaatagcan gcatgctggg ngatgcggtg ggctc
//
