LOCUS       sequence-305625-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of CDK2 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CDK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(126..146)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      147..156
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtgttttgtg agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
       61 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
      121 acggccaact tttgtataca aagtgggcca ccatgggaat cccgctgctg aacgctaaac
      181 agattctggg acagctggac ggcgggagcg tggcagtcct gcctgtggtc gactccacca
      241 atcagtacct gctggatcga atcggcgagc tgaagagtgg ggatgcttgc attgcagaat
      301 atcagcaggc agggagagga agcagaggga ggaaatggtt ctctcctttt ggagctaacc
      361 tgtacctgag tatgttttgg cgcctgaagc ggggaccagc agcaatcggc ctgggcccgg
      421 tcatcggaat tgtcatggca gaagcgctgc gaaagctggg agcagacaag gtgcgagtca
      481 aatggcccaa tgacctgtat ctgcaggata gaaagctggc aggcatcctg gtggagctgg
      541 ccggaataac aggcgatgct gcacagatcg tcattggcgc cgggattaac gtggctatga
      601 ggcgcgtgga ggaaagcgtg gtcaatcagg gctggatcac actgcaggaa gcagggatta
      661 acctggacag gaatactctg gccgctatgc tgatccgaga gctgcgggca gccctggaac
      721 tgttcgagca ggaaggcctg gctccatatc tgtcacggtg ggagaagctg gataacttca
      781 tcaatagacc cgtgaagctg atcattgggg acaaagagat tttcgggatt agccggggga
      841 ttgataaaca gggagccctg ctgctggaac aggacggagt tatcaaaccc tgg
//
