LOCUS       sequence-305626-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of CDK2 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CDK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..136
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgataaacag ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg
       61 agaaatcagt ctgcggtctg ccgaaaaggc tagcggcaag cccatcccca accccctgct
      121 gggcctggac agcaccacaa gtttgtacaa aaaagcaggc tccatcatgg agaacttcca
      181 aaaggtggaa aagatcggag agggcacgta cggagttgtg tacaaagcca gaaacaagtt
      241 gacgggagag gtggtggcgc ttaagaaaat ccgcctggac actgagactg agggtgtgcc
      301 cagtactgcc atccgagaga tctctctgct taaggagctt aaccatccta atattgtcaa
      361 gctgctggat gtcattcaca cagaaaataa actctacctg gtttttgaat ttctgcacca
      421 agatctcaag aaattcatgg atgcctctgc tctcactggc attcctcttc ccctcatcaa
      481 gagctatctg ttccagctgc tccagggcct agctttctgc cattctcatc gggtcctcca
      541 ccgagacctt aaacctcaga atctgcttat taacacagag ggggccatca agctagcaga
      601 ctttggacta gccagagctt ttggagtccc tgttcgtact tacacccatg aggtggtgac
      661 cctgtggtac cgagctcctg aaatcctcct gggctgcaaa tattattcca cagctgtgga
      721 catctggagc ctgggctgca tctttgctga gatggtgact cgccgggccc tattccctgg
//
