LOCUS       sequence-30564-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: PCDNA3 T7 DLC2
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             78..110
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(444..462)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(445..462)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(482..499)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    488..712
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(845..864)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actctctggc tactgagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttatg gctagcatga ctggtggaca gcaaatgggt ggatcctctg
      121 accggaaggc agtgatcaag aacgcagaca tgtctgagga catgcaacag gatgccgttg
      181 actgtgccac acaggccatg gagaagtaca acatagagaa ggacattgct gcctatatca
      241 agaaggaatt tgacaagaaa tataacccta cctggcattg tatcgtgggc cggaattttg
      301 gcagctacgt cacacacgag acaaagcact tcatctattt ttacttgggt caagttgcaa
      361 tcctcctctt caagtcgggc taggaattct gcagatatcc atcacactgg cggccgctcg
      421 agcatgcatc tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca
      481 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
      541 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
      601 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
      661 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
      721 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
      781 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      841 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      901 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctc
//
