LOCUS       sequence-305642-       11496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible Bxb1 integrase for mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11496)
  AUTHORS   Chow KK, Budde MW, Granados AA, Cabrera M, Yoon S, Cho S, Huang TH, 
            Koulena N, Frieda KL, Cai L, Lois C, Elowitz MB
  TITLE     Imaging cell lineage with a synthetic digital recording system.
  JOURNAL   Science. 2021 Apr 9;372(6538). pii: 372/6538/eabb3099. doi: 
            10.1126/science.abb3099.
  PUBMED    33833095
REFERENCE   2  (bases 1 to 11496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2021-04-9"; volume: "372(6538). pii: 372/6538/eabb3099. doi";
            pages: " 10.1126/science.abb3099"
COMMENT     Sequence Label: TIGRE-TRE-[poor kozak]Bxb1-ecDHFR-BGHpA
FEATURES             Location/Qualifiers
     source          1..11496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    414..447
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     promoter        3001..3379
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    3009..3027
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3045..3063
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3081..3099
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3117..3135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3153..3171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3189..3207
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3225..3243
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3288..3312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4928..5404
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDYVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     polyA_signal    5437..5661
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8128..8627
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8163..8186)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8560..8579
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(9275..9291)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9275..9291)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9288..9310)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9299..9315
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9323..9329,9330..9347,9348..9353))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9368..9389
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9506..9523)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9677..10265)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9757..9776)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10436..11227,11228..11296))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11059..11078
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11297..11401)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11469..11487
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgaagttc
      421 ctattcttca aatagtatag gaacttcttt cggccggcca atcgattggt tttatgccgc
      481 ggttaccgtg aagatatctt aattaagaat tcgagctcgc tagagcccca tcctcactga
      541 ctccgtcctg gagttggatg agagataatg gccttacgtt gtgccagggg agggtcgggc
      601 tggatttagc aagatttacc ttctccaaag agcggtgctg cagtggcaca gctgcccacg
      661 gaggtggggg ggtcaccgtc cctggaggtg atgaagaact gtggggatgt ggcactgagg
      721 gacatggcca gtgggcacgg tgggtgggtt ggggttggtc ttggggatct tggagggctt
      781 ttccagcctt catgatttga cgattgtatg aacatctaca tggcaattct ccagctgcct
      841 gtcccagtcc tactgaccca gctgtatctc tccaggcaag ctcttccacc ccttctgctt
      901 gcatccagac accatcaaac atgcaggctc agacacaggg accagcagtg tctgtggcct
      961 ttttgtgctc ctctccatgc tgggttttaa cttgctcttt gtccttctat cctatcttct
     1021 tatccttaag gctgttctga acgctgtgac ttggagagtg tcccagagcc ctcaacacct
     1081 gcatgtccca cgtccatgct gtcctgcact tccttatccc caagatctgc ctctccgtga
     1141 tgcactgaat tggcaaacat gtgtcacccc agaccaacaa tgtcacagca aactccccct
     1201 tgataggaca agggggaatg gctttacact gagacagggg aggtttgggt tggatatgag
     1261 gaggcagttt ttcccccaga gggtggtgac gcactgaaca ggttgcccaa ggaggctgtg
     1321 gatgccccat ccctgcaggc attcaaggcc aggctggatg tggctctggg cagcctgggc
     1381 tgctggttga tgaccctgca catagcaggg ggttggatct ggatgagcac tgtgctcctt
     1441 tgcaacccag gccgttctat gattctgtca ttctaaatct ctctttcagc ctaaagcttt
     1501 ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa
     1561 agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga
     1621 tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg
     1681 gggagggacg taattacatc cctgggggct ttgggggggg gctgtcccga gctcggtacc
     1741 gccccatcct cactgactcc gtcctggagt tggatgagag ataatggcct tacgttgtgc
     1801 caggggaggg tcgggctgga tttagcaaga tttaccttct ccaaagagcg gtgctgcagt
     1861 ggcacagctg cccacggagg tgggggggtc accgtccctg gaggtgatga agaactgtgg
     1921 ggatgtggca ctgagggaca tggccagtgg gcacggtggg tgggttgggg ttggtcttgg
     1981 ggatcttgga gggcttttcc agccttcatg atttgacgat tgtatgaaca tctacatggc
     2041 aattctccag ctgcctgtcc cagtcctact gacccagctg tatctctcca ggcaagctct
     2101 tccacccctt ctgcttgcat ccagacacca tcaaacatgc aggctcagac acagggacca
     2161 gcagtgtctg tggccttttt gtgctcctct ccatgctggg ttttaacttg ctctttgtcc
     2221 ttctatccta tcttcttatc cttaaggctg ttctgaacgc tgtgacttgg agagtgtccc
     2281 agagccctca acacctgcat gtcccacgtc catgctgtcc tgcacttcct tatccccaag
     2341 atctgcctct ccgtgatgca ctgaattggc aaacatgtgt caccccagac caacaatgtc
     2401 acagcaaact cccccttgat aggacaaggg ggaatggctt tacactgaga caggggaggt
     2461 ttgggttgga tatgaggagg cagtttttcc cccagagggt ggtgacgcac tgaacaggtt
     2521 gcccaaggag gctgtggatg ccccatccct gcaggcattc aaggccaggc tggatgtggc
     2581 tctgggcagc ctgggctgct ggttgatgac cctgcacata gcagggggtt ggatctggat
     2641 gagcactgtg ctcctttgca acccaggccg ttctatgatt ctgtcattct aaatctctct
     2701 ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga
     2761 gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac
     2821 gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc
     2881 tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg
     2941 tccctctagg ggtacccggg gatccggtct cgcgtaccct ttcgagttca agaattcctc
     3001 gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag
     3061 agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc
     3121 tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta
     3181 cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag
     3241 agaacgtata agctttaggc gtgtacggtg ggcgcctata aaagcagagc tcgtttagtg
     3301 aaccgtcaga tcgcctggag caattccaca acacttttgt cttataccaa ctttccgtac
     3361 cacttcctac cctcgtaaag tcgacggatc cgtttaaagg tgcatgaggg cccttgtagt
     3421 tatcaggttg agtagggtta cggatgcaac caccagcccg gagcgccaac tggaatcatg
     3481 tcagcagctt tgtgcgcagc gcggctggga cgtggtggga gtggcggagg acttggacgt
     3541 tagcggggcc gttgacccat ttgaccgaaa gcggagacct aacctggctc gatggctcgc
     3601 ctttgaggag cagcccttcg atgtgatcgt cgcatacagg gtcgacagac tgaccagatc
     3661 cattcgccat ctgcagcagc tcgttcactg ggcggaggac cacaaaaagc tcgtggtgag
     3721 tgcaacagaa gcccactttg acaccacaac acccttcgca gccgtcgtga tcgctctgat
     3781 gggtaccgtt gcccagatgg aattggaggc aatcaaggag cggaacagat ccgccgctca
     3841 tttcaatatc cgcgcgggca agtacagggg tagtctccca ccctgggggt atttgcctac
     3901 ccgggtggac ggcgaatgga ggcttgttcc cgatcccgtg cagcgagagc gaatactgga
     3961 agtttatcat cgagtcgtgg ataaccatga accactccac ctggtggccc acgaccttaa
     4021 ccgacgcggc gtgctgagcc ctaaggacta ttttgctcaa cttcagggaa gagagccaca
     4081 gggtagggaa tggtcagcca cagctctcaa gcggtctatg atttccgaag caatgctcgg
     4141 gtacgcaaca ctcaatggca agacagttcg agacgacgac ggggcccccc tggttcgggc
     4201 cgaacccata cttacccgcg aacaactgga ggcacttcgc gcggaacttg tgaaaacaag
     4261 ccgagccaaa cccgcagtga gcaccccatc actgctgctg agggtgctct tctgtgccgt
     4321 gtgcggcgaa ccagcataca agttcgctgg cgggggtcga aaacaccccc gctaccggtg
     4381 tcgctcaatg ggttttccaa agcactgtgg caacggaaca gttgcaatgg ccgaatggga
     4441 cgctttttgt gaagaacaag tgctggatct tctgggcgac gctgagaggc tggaaaaagt
     4501 atgggtggcc gggagcgaca gcgccgttga gctcgccgag gtgaacgccg aattggtgga
     4561 cctgacgagt ctcatcggat ctccagcata ccgagctgga tccccccagc gagaggctct
     4621 ggacgctcgg atagccgccc tggcagcaag gcaggaggag cttgaggggt tggaagcacg
     4681 gccttcagga tgggaatggc gggaaacagg acagagattt ggagactggt ggagggaaca
     4741 ggataccgct gctaagaaca cttggctcag gtccatgaat gttcgactca ccttcgacgt
     4801 gaggggtggg ttgacccgca ccattgattt cggggatctg caggagtatg aacagcatct
     4861 ccggcttggc tccgtggtag aaagacttca tacaggcatg tcaggtggat caggtggagg
     4921 cagtggcatg atcagtctga ttgcggcgtt agcggtagat tacgttatcg gcatggaaaa
     4981 cgccatgccg tggaacctgc ctgccgatct cgcctggttt aaacgcaaca ccttaaataa
     5041 acccgtgatt atgggccgcc atacctggga atcaatcggt cgtccgttgc caggacgcaa
     5101 aaatattatc ctcagcagtc aaccgagtac ggacgatcgc gtaacgtggg tgaagtcggt
     5161 ggatgaagcc atcgcggcgt gtggtgacgt accagaaatc atggtgattg gcggcggtcg
     5221 cgttattgaa cagttcttgc caaaagcgca aaaactgtat ctgacgcata tcgacgcaga
     5281 agtggaaggc gacacccatt tcccggatta cgagccggat gactgggaat cggtattcag
     5341 cgaattccac gatgctgatg cgcagaactc tcacagctat tgctttgaga ttctggagcg
     5401 gcgagcatgc taaacctcga gaccacctgc taaggtctgt gccttctagt tgccagccat
     5461 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     5521 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     5581 ggggtggggt ggggcaggac agcaaggggg aggattggga agagaatagc aggcatgctg
     5641 gggatgcggt gggctctatg gtactctagt ctagagcccc atcctcactg actccgtcct
     5701 ggagttggat gagagataat ggccttacgt tgtgccaggg gagggtcggg ctggatttag
     5761 caagatttac cttctccaaa gagcggtgct gcagtggcac agctgcccac ggaggtgggg
     5821 gggtcaccgt ccctggaggt gatgaagaac tgtggggatg tggcactgag ggacatggcc
     5881 agtgggcacg gtgggtgggt tggggttggt cttggggatc ttggagggct tttccagcct
     5941 tcatgatttg acgattgtat gaacatctac atggcaattc tccagctgcc tgtcccagtc
     6001 ctactgaccc agctgtatct ctccaggcaa gctcttccac cccttctgct tgcatccaga
     6061 caccatcaaa catgcaggct cagacacagg gaccagcagt gtctgtggcc tttttgtgct
     6121 cctctccatg ctgggtttta acttgctctt tgtccttcta tcctatcttc ttatccttaa
     6181 ggctgttctg aacgctgtga cttggagagt gtcccagagc cctcaacacc tgcatgtccc
     6241 acgtccatgc tgtcctgcac ttccttatcc ccaagatctg cctctccgtg atgcactgaa
     6301 ttggcaaaca tgtgtcaccc cagaccaaca atgtcacagc aaactccccc ttgataggac
     6361 aagggggaat ggctttacac tgagacaggg gaggtttggg ttggatatga ggaggcagtt
     6421 tttcccccag agggtggtga cgcactgaac aggttgccca aggaggctgt ggatgcccca
     6481 tccctgcagg cattcaaggc caggctggat gtggctctgg gcagcctggg ctgctggttg
     6541 atgaccctgc acatagcagg gggttggatc tggatgagca ctgtgctcct ttgcaaccca
     6601 ggccgttcta tgattctgtc attctaaatc tctctttcag cctaaagctt tttccccgta
     6661 tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga aagcgatccc
     6721 gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg atgcgggggg
     6781 agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac
     6841 gtaattacat ccctgggggc tttggggggg ggctgtccct ctagagcccc atcctcactg
     6901 actccgtcct ggagttggat gagagataat ggccttacgt tgtgccaggg gagggtcggg
     6961 ctggatttag caagatttac cttctccaaa gagcggtgct gcagtggcac agctgcccac
     7021 ggaggtgggg gggtcaccgt ccctggaggt gatgaagaac tgtggggatg tggcactgag
     7081 ggacatggcc agtgggcacg gtgggtgggt tggggttggt cttggggatc ttggagggct
     7141 tttccagcct tcatgatttg acgattgtat gaacatctac atggcaattc tccagctgcc
     7201 tgtcccagtc ctactgaccc agctgtatct ctccaggcaa gctcttccac cccttctgct
     7261 tgcatccaga caccatcaaa catgcaggct cagacacagg gaccagcagt gtctgtggcc
     7321 tttttgtgct cctctccatg ctgggtttta acttgctctt tgtccttcta tcctatcttc
     7381 ttatccttaa ggctgttctg aacgctgtga cttggagagt gtcccagagc cctcaacacc
     7441 tgcatgtccc acgtccatgc tgtcctgcac ttccttatcc ccaagatctg cctctccgtg
     7501 atgcactgaa ttggcaaaca tgtgtcaccc cagaccaaca atgtcacagc aaactccccc
     7561 ttgataggac aagggggaat ggctttacac tgagacaggg gaggtttggg ttggatatga
     7621 ggaggcagtt tttcccccag agggtggtga cgcactgaac aggttgccca aggaggctgt
     7681 ggatgcccca tccctgcagg cattcaaggc caggctggat gtggctctgg gcagcctggg
     7741 ctgctggttg atgaccctgc acatagcagg gggttggatc tggatgagca ctgtgctcct
     7801 ttgcaaccca ggccgttcta tgattctgtc attctaaatc tctctttcag cctaaagctt
     7861 tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga
     7921 aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg
     7981 atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg
     8041 ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtccct ctagaagtcg
     8101 acggcgcggg ccgcatcgaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     8161 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     8221 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     8281 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     8341 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     8401 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     8461 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     8521 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     8581 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcaatcgcc
     8641 gctagccgcc accatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     8701 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     8761 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     8821 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     8881 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     8941 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     9001 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     9061 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     9121 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgtaa gtgctagccc
     9181 ctaggcctga atcgaagttc ctattcttca aaaggtatag gaacttcgag ctcgtcgact
     9241 gcagaggcct gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     9301 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     9361 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     9421 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     9481 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     9541 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     9601 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9661 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9721 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9781 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9841 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9901 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9961 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10021 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    10081 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    10141 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10201 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10261 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    10321 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    10381 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    10441 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    10501 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    10561 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    10621 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    10681 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    10741 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    10801 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    10861 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    10921 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    10981 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    11041 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    11101 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    11161 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    11221 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    11281 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    11341 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    11401 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
    11461 cctataaaaa taggcgtatc acgaggccct ttcgtc
//
