LOCUS       sequence-30565-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 Flag DLC2
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(451..469)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(452..469)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(489..506)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    495..719
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(852..871)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctctctggc tactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttac ggcagccaac atggactata aggacgatga tgacaaagga
      121 tcctctgacc ggaaggcagt gatcaagaac gcagacatgt ctgaggacat gcaacaggat
      181 gccgttgact gtgccacaca ggccatggag aagtacaaca tagagaagga cattgctgcc
      241 tatatcaaga aggaatttga caagaaatat aaccctacct ggcattgtat cgtgggccgg
      301 aattttggca gctacgtcac acacgagaca aagcacttca tctattttta cttgggtcaa
      361 gttgcaatcc tcctcttcaa gtcgggctag gaattctgca gatatccatc acactggcgg
      421 ccgctcgagc atgcatctag agggccctat tctatagtgt cacctaaatg ctagagctcg
      481 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
      541 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
      601 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
      661 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
      721 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
      781 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
      841 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
      901 ccgtcaag
//
