LOCUS       sequence-30567-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 T7 BimL (S44D)
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             89..121
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(602..620)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(603..620)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(640..657)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    646..870
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gctttagcga gctctctggc tactagagaa cccactgctt actggcttat cgaaattaat
       61 acgactcact atagggagac ccaagcttat ggctagcatg actggtggac agcaaatggg
      121 tggatccgca aagcaacctt ctgatgtaag ttctgagtgt gaccgagaag gtagacaatt
      181 gcagcctgcg gagaggcctc cccagctcag acctggggcc cctacctccc tacagacaga
      241 gccacaagac agggacccag cacccatgag ttgtgacaaa tcaacacaaa ccccaagtcc
      301 tccttgccag gccttcaacc actatctcag tgcaatggct tccatgaggc aggctgaacc
      361 tgcagatatg cgcccagaga tatggatcgc ccaagagttg cggcgtatcg gagacgagtt
      421 taacgcttac tatgcaagga gggtattttt gaataattac caagcagccg aagaccaccc
      481 acgaatggtt atcttacgac tgttacgtta cattgtccgc ctggtgtgga gaatgcattg
      541 agaattctgc agatatccat cacactggcg gccgctcgag catgcatcta gagggcccta
      601 ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt
      661 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
      721 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
      781 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
      841 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
      901 ctagggg
//
