LOCUS       sequence-305672-        2110 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BRET biosensor for detection of G-alpha-q-GTP in lentiviral vector 
            pLenti-hSyn.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, 
            Garcia-Marcos M
  TITLE     Revealing the Activity of Trimeric G-proteins in Live Cells with a 
            Versatile Biosensor Design.
  JOURNAL   Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020.
  PUBMED    32634377
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020."
COMMENT     Sequence Label: pLenti-hSyn-G-alpha-q*-BERKY1
FEATURES             Location/Qualifiers
     source          1..2110
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             82..594
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             619..639
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(1201..1220)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1360..1611
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1548..1569
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2067..2096
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gctagcatgg gatgtataaa atcaaaaggg aaagacagca agcttggatc aggcggtagc
       61 gggggatcag gcggtagcgg gatggtcttc acactcgaag atttcgttgg ggactggcga
      121 cagacagccg gctacaacct ggaccaagtc cttgaacagg gaggtgtgtc cagtttgttt
      181 cagaatctcg gggtgtccgt aactccgatc caaaggattg tcctgagcgg tgaaaatggg
      241 ctgaagatcg acatccatgt catcatcccg tatgaaggtc tgagcggcga ccaaatgggc
      301 cagatcgaaa aaatttttaa ggtggtgtac cctgtggatg atcatcactt taaggtgatc
      361 ctgcactatg gcacactggt aatcgacggg gttacgccga acatgatcga ctatttcgga
      421 cggccgtatg aaggcatcgc cgtgttcgac ggcaaaaaga tcactgtaac agggaccctg
      481 tggaacggca acaaaattat cgacgagcgc ctgatcaacc ccgacggctc cctgctgttc
      541 cgagtaacca tcaacggagt gaccggctgg cggctgtgcg aacgcattct ggcggaattc
      601 ggatctggtg gttcaggcga aaacctgtat tttcagggcg gaagcggaga agaggaggaa
      661 aagaaaaaac aacaggagga ggaggcggag aggctgagga ggatacaaga ggaaatggaa
      721 aaagaaagaa agcggcgaga ggaagatgag cagcgacgcc gcaaggagga ggaagagaga
      781 aggatgaaat tggagatgga ggcaaaacgc aaacaagaag aggaagaacg gaagaaacgg
      841 gaggatgatg aaaagcggaa gaaaaaggga tctggagggt cagggggcgc gcccatggtt
      901 tcaaagggag aagagttgtt cacaggggtt gtaccgattc ttgtagaact tgacggagat
      961 gtcaacgggc acaagtttag tgtaagtgga gaaggcgagg gcgatgcgac ctatggaaaa
     1021 ctgaccctta aatttatttg tactacgggt aaattgcccg ttccctggcc gacattggta
     1081 actacattcg gatatggttt gatgtgcttt gcaaggtatc cggatcacat gaagcagcat
     1141 gatttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1201 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1261 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1321 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     1381 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     1441 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1501 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1561 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggatccggt
     1621 ggcaccggtg gatcaggctc ttctcgagag aagtacctgg aggaccgggg cgaggtgact
     1681 tttgagaaga tcttctccca gaagctgggg tacctgcttt tccgagactt ctgcctgaag
     1741 cacctggagg aggccaagcc cttggtagag ttctacgagg agatcaagaa atacgagaag
     1801 ctggagacag aggaggagcg cctggtctgc agccgagaga tcttcgacac gtacatcatg
     1861 aaggagctgc tggcctgctc acatcctttc tcgaagagcg ccattgagca cgtccagggc
     1921 catctggtga agaagcaggt gcctccggat ctcttccagc catatattga agaaatttgc
     1981 cagaacctcc gaggagacgt gttccagaaa ttcatcgaga gcgataaatt cacacggttt
     2041 tgccagtgga agtgactcga ggtggagaac aaaaacttat ttctgaagaa gatctgtgat
     2101 ctagaccggt
//
