LOCUS       sequence-305675-        8671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector for site specific integration of doxycycline 
            inducible expression of full length human SHH cDNA into human AAVS1 
            locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Cederquist GY, Asciolla JJ, Tchieu J, Walsh RM, Cornacchia D, Resh 
            MD, Studer L
  TITLE     Specification of positional identity in forebrain organoids.
  JOURNAL   Nat Biotechnol. 2019 Apr;37(4):436-444. doi: 
            10.1038/s41587-019-0085-3. Epub 2019 Apr 1.
  PUBMED    30936566
REFERENCE   2  (bases 1 to 8671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-019-0085-3"; journalName: "Nat Biotechnol"; date: 
            "2019-04"; volume: "37"; issue: "4"; pages: "436-444"
COMMENT     Sequence Label: AAVS1-TRE-SHH
FEATURES             Location/Qualifiers
     source          1..8671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      21..609
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     510..529
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     763..780
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    897..918
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(933..938,939..956,957..963)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    971..987
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     976..998
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     995..1011
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     995..1011
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1032..1050
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1085..1888
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    1895..1920
                     /label=SA
                     /note="splice acceptor site"
     CDS             1944..1997
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2007..2606
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2007..2026)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2503..2523
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(2638..2655)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2644..2868
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2875..2956
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2900..2919
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    3140..3164
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4587..4611)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(4628..4942)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(4631..4655)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4702..4720
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4738..4756
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4773..4791
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4809..4827
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4845..4863
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4880..4898
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4916..4934
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    4959..5795
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(5829..5848)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5830..5848)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5855..5872)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5855..5871)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5864..5886)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             6009..6311
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6190..6209
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(6714..6733)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7328..7347
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(7708..8499,8500..8568))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8331..8350
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
       61 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
      121 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
      181 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
      241 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
      301 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
      361 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
      421 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
      481 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
      541 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
      601 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      661 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
      721 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
      781 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
      841 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
      901 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
      961 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     1021 gccaagctca gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc
     1081 cctttgcttt ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc
     1141 ttgtggcctg ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt
     1201 ccgtcttcct ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc
     1261 tttccggagc acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc
     1321 cgtgtctggg tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact
     1381 cgctgggttc ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg
     1441 gccttctccg acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat
     1501 caccgttttt ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat
     1561 cctcttgctt tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc
     1621 ctttcatttg ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag
     1681 ccccctgtca tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc
     1741 gggcccctat gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg
     1801 agctgggacc accttatatt cccagggccg gttaatgtgg ctctggttct gggtactttt
     1861 atctgtcccc tccaccccac agtggggcaa gcttctgacc tcttctcttc ctcccacagg
     1921 gcctcgagag atctggcagc ggagagggca gaggaagtct tctaacatgc ggtgacgtgg
     1981 aggagaatcc cggccctagg ctcgagatga ccgagtacaa gcccacggtg cgcctcgcca
     2041 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     2101 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     2161 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     2221 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     2281 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     2341 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     2401 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     2461 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     2521 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     2581 gcatgacccg caagcccggt gcctgatcta gagggcccgt ttaaacccgc tgatcagcct
     2641 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     2701 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     2761 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     2821 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatgggt cgaggcagtg
     2881 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     2941 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     3001 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     3061 tcctctagag atatcgtcga caagcttatc gatgcggccg cgctagcacg cgtcagctga
     3121 ctagaggatc cccggatcaa ccactttgta caagaaagct gggttgcggc cgcactcgag
     3181 ctagctggac ttgaccgcca tgcccagcgg gtgcagggcc tcgctgtcca ggagccaggt
     3241 gcctatttgg tagagcagct gcgagtacca gtggatgccc gcggtggccc ccgcacccgg
     3301 agcgtcggca gcacctggag cggttagggc tactctgccg ccgccgcccc cgcggtcccc
     3361 gccgccgctg tccccgccgc ggtccgtgcg cgcgggcgcc agtgcagcca ggagcgcgtg
     3421 cgccaggcgg aagggcgcga aggcccggtg cgcccagctg tgctcctcga tgaccgcgta
     3481 gcacgaggcc agcacccggt tgatgagaat ggtgccctgg gccgtgagcg gcgcgtaggc
     3541 gcccgcggcc tcctcgctta gggtcacgct gtgcacagcg gcgggcagga gccggcggtc
     3601 cccgtcacgc tcggccacca cgtacacgcg ctggcccggg cgcacgcggc tggcgaacag
     3661 cgcccgaggc cccagtgcgc ccccggaagg cggccccgag cccgaggacg cctcgggctc
     3721 cccggtggcc gagtcgttgt gcggcgccac aaagagcagg tgcgcggcgg tgagcagcag
     3781 gcgctcgcgc ggctcccgcg tctcgatcac gtagaagacc ttcttggcgc cgtcgtcgcg
     3841 gtccaggaaa gtgaggaagt cgctgtagag cagccggccc tggtcgtccg ccgccagcac
     3901 gcggtccccg gggctcaggt ccttcaccag cttggtgccg ccctgctcca ggtgcaccgt
     3961 ggccgagccc gggaagcagc ctcccgattt ggccgccacc gagttctctg ctttcaccga
     4021 gcagtggata tgtgccttgg actcgtagta cacccagtcg aagccggcct ccaccgccag
     4081 gcgggccagc atgccgtact tgctgcggtc gcggtcagac gtggtgatgt ccactgcgcg
     4141 gccctcgtag tgcagagact cctctgagtg gtggccatct tcgtcccagc cctcggtcac
     4201 ccgcagtttc actcctggcc actggttcat caccgagatg gccaaagcgt tcaacttgtc
     4261 cttacacctc tgagtcatca gcctgtccgc tccggtgttt tcttcatcct taaatatgat
     4321 gtcggggttg taattggggg tgagttcctt aaatcgctcg gagtttctgg agatcttccc
     4381 ttcatacctt ccgctggcgc ctagggtctt ctcggccaca ttggggataa actgcttgta
     4441 ggctaaaggg gtcagctttt tggggtgcct cctcttcccg aaccccctgc ccggtccgca
     4501 cgccagtccc gagcatacca gcagcgagga gacgaggact agcagcagac atctcgccag
     4561 cagcagcatg gtaccgaatt ccttcaagcc tgcttttttg tacaaacttg ttgatcggaa
     4621 ttctccaggc gatctgacgg ttcactaaac gagctctgct tatataggcc tcccaccgta
     4681 cacgcctacc tcgacatacg ttctctatca ctgataggga gtaaactcga catacgttct
     4741 ctatcactga tagggataaa ctcgacatac gttctctatc actgataggg agtaaactcg
     4801 acatacgttc tctatcactg atagggagta aactcgacat acgttctcta tcactgatag
     4861 ggagtaaact cgacatcgtt ctctatcact gatagggagt aaactcgaca tacgttctct
     4921 atcactgata gggagtaaac tcgacagtac taagctttac tagggacagg attggtgaca
     4981 gaaaagcccc atccttaggc ctcctccttc ctagtctcct gatattgggt ctaaccccca
     5041 cctcctgtta ggcagattcc ttatctggtg acacaccccc atttcctgga gccatctctc
     5101 tccttgccag aacctctaag gtttgcttac gatggagcca gagaggatcc tgggagggag
     5161 agcttggcag ggggtgggag ggaagggggg gatgcgtgac ctgcccggtt ctcagtggcc
     5221 accctgcgct accctctccc agaacctgag ctgctctgac gcggctgtct ggtgcgtttc
     5281 actgatcctg gtgctgcagc ttccttacac ttcccaagag gagaagcagt ttggaaaaac
     5341 aaaatcagaa taagttggtc ctgagttcta actttggctc ttcacctttc tagtccccaa
     5401 tttatattgt tcctccgtgc gtcagtttta cctgtgagat aaggccagta gccagccccg
     5461 tcctggcagg gctgtggtga ggaggggggt gtccgtgtgg aaaactccct ttgtgagaat
     5521 ggtgcgtcct aggtgttcac caggtcgtgg ccgcctctac tccctttctc tttctccatc
     5581 cttctttcct taaagagtcc ccagtgctat ctgggacata ttcctccgcc cagagcaggg
     5641 tcccgcttcc ctaaggccct gctctgggct tctgggtttg agtccttggc aagcccagga
     5701 gaggcgctca ggcttccctg tcccccttcc tcgtccacca tctcatgccc ctggctctcc
     5761 tgccccttcc ctacaggggt tcctggctct gctctaaggg cgaattcgcg gccgctaaat
     5821 tcaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg
     5881 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     5941 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctat
     6001 acgtacggca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     6061 atgtacagag tgatattatt gacacgccgg ggcgacggat ggtgatcccc ctggccagtg
     6121 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     6181 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
     6241 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
     6301 ggggaatata aatgtcaggc atgagattat caaaaaggat cttcacctag atccttttca
     6361 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
     6421 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     6481 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     6541 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     6601 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca
     6661 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     6721 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     6781 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     6841 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     6901 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     6961 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     7021 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     7081 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     7141 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     7201 gcgaggatct cgtcgtgacc catgcatggc gatgcctgct tgccgaatat catggtggaa
     7261 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     7321 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     7381 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     7441 cttgacgagt tcttctgaat tattaacgct tacaatttcc tgatgcggta ttttctcctt
     7501 acgcatctgt gcggtatttc acaccgcatc aggtggcact tttcggggaa atgtgcgcgg
     7561 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagattatc
     7621 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7681 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7741 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7801 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7861 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7921 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7981 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8041 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8101 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8161 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8221 tgtcatgcca tccgtaagat gcttttctgt gactggtgaa tactcaacca agtcattctg
     8281 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8341 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8401 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8461 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8521 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8581 tcaatattat tgaagcattt atcagggtta ttgtctcatg accaaaatcc cttaacgtga
     8641 gttttcgttc cactgagcgt cagaccccgt a
//
