LOCUS       sequence-305676-       10205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffold.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Alda-Catalinas C, Bredikhin D, Hernando-Herraez I, Santos F, 
            Kubinyecz O, Eckersley-Maslin MA, Stegle O, Reik W
  TITLE     A Single-Cell Transcriptomics CRISPR-Activation Screen Identifies 
            Epigenetic Regulators of the Zygotic Genome Activation Program.
  JOURNAL   Cell Syst. 2020 Jul 22;11(1):25-41.e9. doi: 
            10.1016/j.cels.2020.06.004. Epub 2020 Jul 6.
  PUBMED    32634384
REFERENCE   2  (bases 1 to 10205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2020.06.004"; journalName: "Cell Syst"; date: 
            "2020-07-22- 22"; volume: "11"; issue: "1"; pages: "25-41.e9"
COMMENT     Sequence Label: CROP-sgRNA-MS2
FEATURES             Location/Qualifiers
     source          1..10205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..69,70..861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(219..238)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1032..1620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1521..1540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1774..1791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1908..1929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1944..1949,1950..1967,1968..1974)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1982..1998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1987..2009
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2006..2022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2006..2022
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2041..2061
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2043..2061
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2089..2315
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2316..2496
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2543..2668
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3161..3394
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3579..3623
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3804..3921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4060..5238
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4291..5229
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5186..5206
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5245..5254
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5251..5958
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5396..5414)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             5968..6021
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6022..6621
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6022..6041)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6518..6538
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6637..7225
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6690..6710)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7108..7119)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7381..7629
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7381..7401
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7552..7571
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8696..8714
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8766..8784
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     LTR             8881..9061
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    9139..9260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9176..9195)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9230..9249
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9279..9413
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9433..9452)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9434..9452)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9462..9479)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9462..9478)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9471..9493)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9620..10075
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9707..9726)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9917..9938
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10101..10205
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
       61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      541 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
      961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1141 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1681 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     1741 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     1801 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1861 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     1921 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     1981 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     2041 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     2101 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     2161 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     2221 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     2281 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     2341 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     2401 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     2461 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     2521 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     2581 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     2641 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     2701 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     2761 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     2821 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     2881 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     2941 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     3001 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     3061 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     3121 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     3181 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     3241 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     3301 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     3361 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     3421 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     3481 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     3541 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     3601 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     3661 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     3721 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag aatcttgaga
     3781 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     3841 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     3901 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc
     3961 cggctcgagg gggcccgggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     4021 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     4081 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgatcc
     4141 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     4201 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     4261 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     4321 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     4381 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     4441 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     4501 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     4561 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     4621 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     4681 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     4741 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     4801 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     4861 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     4921 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     4981 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     5041 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     5101 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     5161 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     5221 ccatttcagg tgtcgtgagg atccgccacc atggtgagca agggcgagga ggataacatg
     5281 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     5341 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     5401 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     5461 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cctgaagctg
     5521 tccttccccg agggcttcaa ttgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     5581 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     5641 ggcaccaact tcccctccga cggccccgtg atgcagtgta ggaccatggg ctgggaggcc
     5701 tccactgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     5761 aagctgaagg acggcggcca ctacgacgct gaggtgaaga ccacctacaa ggccaagaag
     5821 cccgtgcagc tgcccggcgc ctacaacgtg gacatcaagc tggacatcct gtcccacaac
     5881 gaggactaca ccatcgtgga gcagtacgag cgcgccgagg gccgccactc caccggcggc
     5941 atggacgagc tgtacaaggg cagcggagaa ggtagaggtt ctctcctcac ttgtggtgat
     6001 gttgaagaaa accctggtcc aatgaccgag tacaagccca cggtgcgcct cgccacccgc
     6061 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     6121 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     6181 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg
     6241 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     6301 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     6361 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggagt ctcgcccgac
     6421 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6481 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6541 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6601 acccgcaagc ccggtgcctg aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     6661 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6721 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6781 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6841 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6901 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6961 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7021 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     7081 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7141 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7201 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     7261 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7321 gaagacaaga tttcaccatt atcgtttcag acccacctcc caaccccgag gggacccaga
     7381 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
     7441 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
     7501 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
     7561 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     7621 cgaaacaccg gagacggttg taaatgagca cacaaaatac acatgctaaa atattatatt
     7681 ctatgacctt tataaaatca accaaaatct tctttttaat aactttagta tcaataatta
     7741 gaatttttat gttccttttt gcaaactttt aataaaaatg agcaaaataa aaaaacgcta
     7801 gttttagtaa ctcgcgttgt tttcttcacc tttaataata gctactccac cacttgttcc
     7861 taagcggtca gctcctgctt caatcatttt ttgagcatct tcaaatgttc taactccacc
     7921 agctgcttta actaaagcat tgtctttaac aactgacttc attagtttaa catcttcaaa
     7981 tgttgcacct gattttgaaa atcctgttga tgttttaaca aattctaatc cagcttcaac
     8041 agctatttca caagctttca tgatttcttc ttttgttaat aaacaatttt ccataataca
     8101 tttaacaaca tgtgatccag ctgctttttt tacagctttc atgtcttcta aaactaattc
     8161 ataatttttg tcttttaatg caccaatatt taataccata tcaatttctg ttgcaccatc
     8221 tttaattgct tcagaaactt cgaatgcttt tgtagctgtt gtgcatgcac ctagaggaaa
     8281 acctacaaca tttgttattc ctacatttgt gccttttaat aattctttac aatagcttgt
     8341 tcaatatgaa ttaacacaaa ctgttgcaaa atcaaattca attgcttcat cacataattg
     8401 tttaatttca gctttcgtag catcttgttt taataatgtg tgatctatat atttgtttag
     8461 tttcattttt tctcctatat attcattttt aattttaatt ctttaataat ttcgtctact
     8521 ttaactttag cgttttgaac agattcacca acacctataa aataaatttt tagtttaggt
     8581 tcagttccac ttgggcgaac agcaaatcat gacttatctt ctaaataaaa ttttagtaag
     8641 tcttgtcctg gcatattata cattccatcg acgtctctgt ttaagagcta ggccaacatg
     8701 aggatcaccc atgtctgcag ggcctagcaa gtttaaataa ggctagtccg ttatcaactt
     8761 ggccaacatg aggatcaccc atgtctgcag ggccaagtgg caccgagtcg gtgctttttt
     8821 aagcttggcg caccaagctt ggcgtaacta gatcttgaga cactgctttt tgcttgtact
     8881 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8941 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     9001 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     9061 agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     9121 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     9181 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     9241 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgcccg
     9301 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9361 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     9421 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     9481 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     9541 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     9601 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9661 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9721 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9781 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9841 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9901 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9961 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    10021 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
    10081 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    10141 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    10201 agagt
//
