LOCUS       sequence-305678-       14436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector to Sox17 gene locus for induction of P2A-fused 
            phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by 
            FRT(F5) sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14436)
  AUTHORS   Serizawa T, Isotani A, Matsumura T, Nakanishi K, Nonaka S, Shibata 
            S, Ikawa M, Okano H
  TITLE     Developmental analyses of mouse embryos and adults using a 
            non-overlapping tracing system for all three germ layers.
  JOURNAL   Development. 2019 Nov 4;146(21). pii: dev.174938. doi: 
            10.1242/dev.174938.
  PUBMED    31597657
REFERENCE   2  (bases 1 to 14436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2019-11-4"; volume: "146(21). pii: 
            dev.174938. doi"; pages: " 10.1242/dev.174938"
COMMENT     Sequence Label: Sox17(2A-phiC31o)
FEATURES             Location/Qualifiers
     source          1..14436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1..792,793..861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     624..643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             6776..6832
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6836..6856
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6857..8671
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             8672..8692
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8740..8788
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(8795..9394)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8878..8898)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9375..9394
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(9413..9460)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(9472..9971)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9520..9539)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     9913..9936
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(12931..12947)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12931..12947)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12944..12966)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12955..12971
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12979..12985,12986..13003,13004..13009))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13024..13045
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        13104..13299
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      13150..13285
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    13305..13439
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13342..13361)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13396..13415
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13507..13524)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13678..14266)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13758..13777)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatac cggttactga gctgggaagt
      901 ggagcgggag cagtttactt cctgcttcgc cgcggaaaaa ctcagctagg gccgagcggg
      961 aaggaggacg tggtctccga gtcgccagct gctagggtgg ggtttcgggg cacgattaga
     1021 cagagattcg gacagttacg tgagaccccg cccacccagg atgcacgctc ccaacggtgt
     1081 gcggaatgag cccccgaagt taatcttggg gtcagacgtt ctcagctctc acgggagagg
     1141 ctagcaaagc gaagattgca gggtctggcc tgaatgttct gggccaccgt gggggccggt
     1201 gatcactgtc tttcttgcca ctctttcctc tcaagttccc gaatttccat ctccacctcc
     1261 gacccactgg ggactgacca gggactggaa agagcgcgaa aggtataaag gggagaagag
     1321 actgcgcctt ttggtgcccg ggtgaaaggt gccaatcgac cgcatcgctc tgcgtggccc
     1381 tacctgggag gtgcggagcc cggggcacga cgctcggtgt tctcagaggc agactctgag
     1441 gcacgtattt tagaccgtcc agtggtacta ttgtctctgt tatcttagag tccgccgggg
     1501 gctgtctccg tgatgacagc ccacgctccc ttttccgccc tcatgaactt gctctccaca
     1561 gaccacggat ctgcttgagt gcccacggat cctgtgcaat tggacttgaa tgtcctttaa
     1621 caggccttgg ccatcagaac accaaaacca taaaaccttc gagaaatcaa ccactcactc
     1681 tgaggctcgc tgtagaagag tggcttagac tctcccactt cggagtccca gttgatcacc
     1741 aacccgcttg ctacaggccc caaatggtta cgggcacagc ttctagttac attagacgct
     1801 tccttgcccc agcctttcga gcctcaggtc tgctttgggg atgtggctta ggcagtagcc
     1861 acctagaaat cctgcactgt ttgagtgtga taccgctgcg gactacagca gacaggcgtg
     1921 ccaggcttct agtccagata cccaccccca ccccagtaaa tctctagtgt tgagggaagc
     1981 aaggcttgca ggaagccgct ccggatgttt gcttctttac ttgtggcatt tgtggctggc
     2041 tttgataacg tcgtgagctc ggcttccaac acccatttag tgaagaaact gaaatatggc
     2101 ccactcacac tgctggcggg tctgaagtgc ggttggcccc aacactcctc ccaaagtatc
     2161 tatcaagaga atggtcagca gaagttagat ctaggtaaga ggacgggaag ggggactagt
     2221 cttgggaaag cgccacattt cctggtagat ttgttaagta aagtgctatc tagggactcc
     2281 aggggaggct gttttaagga acggggaaga gcccaaaagt ggtcgcttgg actcctactg
     2341 tgtttgtgct ttccctcaga aagatcagtt gttaatcttc agggatgctt gtaaataacg
     2401 ctgcctcccc atccccgggc tttctttcta accactttct cagttccttt tatggagctc
     2461 gaacagaact aggtacccag gtaaaatggt gtaaatacat tttctagacc tacacctgaa
     2521 ttatatttct gttgtctctt agtttttaga gaaatcaacc aagacaggga agacagagaa
     2581 aatgcattct ggacccgcta ctgttttcaa tcgtcatttc aaacaaaacc cgtagtttat
     2641 ttaaaaaaaa aaaaaacacg gaaagagaaa attaggccct gagatctttg aaaagatgcc
     2701 cattactaga tacaaaagag aagagtcact gtggaggtga gggactgcgt ccctgcttct
     2761 gagaggcagg agagctacat ggggagactg tctcaaaact aacaacagaa gagatagaat
     2821 tttttttttc ttaaagagca cctggctaga gaatcgattt gtaaaattgc tatttctacc
     2881 cttagagttt ccttgattaa acatctgaaa tgcatttttt ttgttttact ttcaagctaa
     2941 tgtttttcag aaaaaaataa aaagctttga ggaaaagcac attatcagtt agatttaaga
     3001 ccaattgctt ttaaattacc aatttaaaat atcatttttt ttatttctta aagcagattt
     3061 aattttaccc cctttgtatg aatcttacag acctggagtt ttaactacta tcactgatct
     3121 ctacacacaa cctccagctt taaatgggag ggagggtcac cactgcttta taacataaca
     3181 aattagagaa aagctggctt ctgtatgagt tctttggaga caaagtagct ccagaaactg
     3241 cagaccagaa gctatcaaaa tctctgtgct tttttggaac ctccagtaag ccagatttgg
     3301 tctctgaaat aatacaggaa aacctcagca tgtcacctca tggatacaag taagtaatac
     3361 ttttcaatca gctattcaca gtaaagaaag tctgttttgg aaactgacat gttgagtcct
     3421 tccttccttc cttccttcct tccttccttc cttccttcct tccttcctcc ctctctccct
     3481 ccctcccttc cttcctttct tcctttcttc ctctccttca agacagggtt gcatgtagcc
     3541 caggctggcc tctaattcca tatgtagcta aggacaatca tgaacgtatt tttctgcccc
     3601 ccacatctca agtgctggaa ttatagaatt acaggcacta ggcatctcat agactgtctt
     3661 tcggaattgt actagatgaa cattcaaact gagctcgatc accattcctt tgagttttcc
     3721 taccttaaaa ctacgcaagg atgaccgttg gatctggtag agaacaacac aaacatgcaa
     3781 acaaactcaa atgtaacaca gagcttttgg agctccttgt ctgaaggagg gctgctaagt
     3841 gtgtgtaaca ctctcccagt gtctgcctta acaggtgatg agtctccctt tcctctccta
     3901 gaatctataa tgctgccctt gaaaaagact acgtctagaa ctaaatgagt ttgctaatat
     3961 ttggagggtg ctgagtggtt gctgggataa gcattgtagg aaggcatgtt ttgaagcccg
     4021 tgggaaagta ctttcaggtt taaaataaat ttaagggtgg tcagcttttc attccagtct
     4081 taagaaaacg ttgtaaccgt ctgtcaggct aacagtctta cacgctacgg atttgctgct
     4141 taaatggact gatccttttc ctccgaggaa cagcacagtg ctgctgtctt tgcactttat
     4201 tttacatttt aatctcgtgg tgtttgcgat ggaatcatgg ccttgcaagt ctttagaaat
     4261 aaaaccagat cttgcctctt ttgccgaaca cacaaaagga gcttttgttt tatattttta
     4321 tgtttcacac tgaaattcct cagggttgac ttgagctctt attgagcagt gatatattag
     4381 tgatatagca ttttatgtta aactttgttt tattacaact tgctatttac cttggatatt
     4441 tttattataa tataagcatg atggatgagt tcttgggaga agaacaggat tttttttctc
     4501 tgaaaaatca tgctaagtta attaccttcg gtaatatttt tttaaaagaa tggaataaat
     4561 acctatacct agtatgttga tgtgaacaac cttggacttg ttcttcaatc ttccgttaca
     4621 gtgagcagca cctccagaca tctgaatttc agccttccta tttccccaag aggtcttggc
     4681 gccagcgccc ggctccagcc agttttcccc aaggcaggtg tagccagaac actgccgggg
     4741 gaggggaaag gggcgggtgt gggacttggg actcggacca ctggctgggc gggggcctaa
     4801 ggactagggt ccggtccgcc cccgcctcgc cattggccgc gtccataaag aaaagggccc
     4861 cgcggcccgg ggggacaccc gcagtgtcac tgggtagatc tccagcgcgg attaggcgaa
     4921 gggttaggtc taccaggaga gcagcgggag ggtagcaccc gctcagcttt acgagttcct
     4981 ctgggcatct ccaggctagc ttccgatccc tgcctcaggg tcgggggaag cggcgtgtcc
     5041 cgtggccata gcagagctcg gggtcggtct ggagagccat gagcagcccg gatgcgggat
     5101 acgccagtga cgaccagagc cagccccgga gcgcgcagcc cgcggtgatg gcagggttgg
     5161 gcccctgtcc ctgggccgag tccctgagcc ccctcgggga tgtaaaggtg aaaggcgagg
     5221 tggtggcgag tagcggggcg ccagccggga cgtcgggccg agccaaagcg gagtctcgca
     5281 tccggcggcc gatgaacgcc tttatggtgt gggccaaaga cgaacgcaag cggttggcac
     5341 agcagaaccc agatctgcac aacgcagagc taagcaagat gctaggtgag tcggaccagg
     5401 gggatacagg gcaccggcgc actccagaga atctccttga gccacttcct tgagccaaga
     5461 ggtgtgctgt agactcaatc ccttcttcca gctccctctc tcgccaaagt aacactttgg
     5521 tctttgggcc ataggagcga atgtaagggt ttctcagtat ttcagagagg taacccgcac
     5581 agccctggct cgaccccttc ccagatcagg ggactttggc agtttttcga gttaagggga
     5641 gaaaggtaag ggaggaagcc ccttctaggc gtcctgtaag gacgcgatct aacactttca
     5701 tccgaatagg accgggcttc tcacctgctc gctcagtgag aggttgcacc agagaggggt
     5761 cagggtgccg gagctcactc gggtaaccct gcgcgcctct ccacctttcc ctgcaggcaa
     5821 gtcttggaag gcgttgacct tggcagagaa gcggcccttc gtggaagagg ccgagcggct
     5881 gcgcgtgcag catatgcagg accaccccaa ctacaagtac cggccgcggc ggcgcaagca
     5941 ggtgaagcgc atgaagcggg tggagggagg cttcctgcac gctctcgtcg agccccaggc
     6001 cggcgcgctt ggtcccgagg gcggccgcgt ggccatggat ggcctgggtc tgcctttccc
     6061 ggagccgggc tatccggccg gtcctccgct gatgtctccg cacatgggcc cccactatcg
     6121 ggactgccag ggactgggcg ctcccgcgct cgacggctac cctctgccca ctccggacac
     6181 atccccgctg gatggcgtgg agcaggaccc ggctttcttt gcagccccgc tgccagggga
     6241 ctgcccggcg gccggcacct acacttacgc tccagtctcg gactatgcag tgtccgtaga
     6301 gccgcccgct ggccccatgc gagtggggcc ggacccctcg ggccctgcga tgccggggat
     6361 cctggcgccc cccagcgctc tgcacctgta ctacggcgcg atgggctcgc ccgccgcaag
     6421 tgcggggcgc ggtttccacg cgcaacccca gcagccgctg caaccgcagg caccgccgcc
     6481 gccaccgcag cagcagcacc cagcgcacgg ccccgggcaa ccttcgcccc ctcccgaggc
     6541 tctgccctgc cgggatggca cggaatccaa ccagcccact gagctcctag gggaggtgga
     6601 ccgcacggaa ttcgaacagt atctgccctt tgtgtataag cccgagatgg gtcttcccta
     6661 ccagggacac gactgcggag tgaacctctc agacagccac ggagccattt cctccgtggt
     6721 gtccgacgct agctcagcgg tctactattg caactacccc gacattggaa gcggagctac
     6781 taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac ctgatccaaa
     6841 aaagaagaga aaggtaatgg atacctacgc cggagcctac gacagacaga gccgggagag
     6901 agagaacagc agcgccgcca gccccgccac ccagagaagc gccaacgagg ataaggccgc
     6961 cgatctgcag agagaggtgg agagggacgg cggcagattc agatttgtgg gccacttcag
     7021 cgaggcccct ggcaccagcg ccttcggcac cgccgagaga cccgagttcg agagaatcct
     7081 gaacgagtgt agggccggca ggctgaacat gatcatcgtg tacgacgtgt cccggttcag
     7141 caggctgaag gtgatggacg ccatccctat cgtgtccgag ctgctggccc tgggcgtgac
     7201 catcgtgtcc acccaggaag gcgtctttag acagggcaac gtgatggacc tgatccacct
     7261 gatcatgagg ctggacgcca gccacaagga gagcagcctg aagagcgcca agatcctgga
     7321 caccaagaac ctgcagaggg agctgggcgg ctatgtgggc ggcaaggccc cctacggctt
     7381 cgagctggtg tccgagacca aggagatcac ccggaacggc aggatggtga acgtggtgat
     7441 caacaagctg gcccacagca ccacccccct gaccggcccc ttcgagtttg agcccgacgt
     7501 gatcaggtgg tggtggcggg agatcaagac ccacaagcac ctgcctttca agcccggcag
     7561 ccaggccgcc atccaccccg gcagcatcac cggcctgtgt aagagaatgg acgccgacgc
     7621 cgtgcccacc agaggcgaga ccatcggcaa gaaaaccgcc agcagcgcct gggaccccgc
     7681 caccgtgatg agaatcctga gggaccctag gatcgccggc ttcgccgccg aggtgatcta
     7741 caagaagaag cccgacggca cccccaccac caagatcgag ggctacagaa tccagagaga
     7801 ccccatcacc ctgagacctg tggagctgga ctgtggccct atcatcgagc ctgccgagtg
     7861 gtacgagctg caggcctggc tggacggcag aggcagaggc aagggcctga gcagaggcca
     7921 ggccatcctg agcgccatgg acaagctgta ctgtgagtgt ggcgccgtga tgaccagcaa
     7981 gagaggcgag gagagcatca aggacagcta ccggtgccgg agaagaaagg tggtggaccc
     8041 cagcgcccct ggccagcacg agggcacctg taatgtgagc atggccgccc tggacaagtt
     8101 cgtggccgag cggatcttca acaagatccg gcacgccgag ggcgacgagg agaccctggc
     8161 cctgctgtgg gaggccgcca gaagattcgg caagctgacc gaggcccccg agaagagcgg
     8221 cgagagggcc aacctggtgg ccgagagagc cgacgccctg aacgccctgg aggagctgta
     8281 cgaggacaga gccgccggag cctatgacgg ccctgtgggc aggaagcact tcagaaagca
     8341 gcaggccgcc ctgaccctga gacagcaggg cgccgaggaa agactggccg agctggaggc
     8401 cgccgaggcc cctaagctgc ccctggatca gtggttcccc gaggatgccg acgccgaccc
     8461 caccggcccc aagtcctggt ggggcagagc cagcgtggac gacaagaggg tgttcgtggg
     8521 cctgttcgtg gataagatcg tggtgaccaa gagcaccacc ggcaggggcc agggcacccc
     8581 catcgagaag agagccagca tcacctgggc caagcctccc accgacgacg acgaggatga
     8641 cgcccaggac ggcaccgagg acgtggccgc ccctaagaaa aagcggaaag tgtgatagat
     8701 gaagttccta taccttttga agaataggaa cttcgatccg aacaaacgac ccaacacccg
     8761 tgcgttttat tctgtctttt tattgccgat cccctcaggc accgggcttg cgggtcatgc
     8821 accaggtgcg cggtccttcg ggcacctcga cgtcggcggt gacggtgaag ccgagccgct
     8881 cgtagaaggg gaggttgcgg ggcgcggagg tctccaggaa ggcgggcacc ccggcgcgct
     8941 cggccgcctc cactccgggg agcacgacgg cgctgcccag acccttgccc tggtggtcgg
     9001 gcgagacgcc gacggtggcc aggaaccacg cgggctcctt gggccggtgc ggcgccagga
     9061 ggccttccat ctgttgctgc gcggccagcc gggaaccgct caactcggcc atgcgcgggc
     9121 cgatctcggc gaacaccgcc cccgcttcga cgctctccgg cgtggtccag accgccaccg
     9181 cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag cccgacgcgc gtgaggaaga
     9241 gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg atcgacggtg tggcgcgtgg
     9301 cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc cctggggacg tcgtcgcggg
     9361 tggcgaggcg caccgtgggc ttgtactcgg tcatggttta gttcctcacc ttgtcgtatt
     9421 atactatgcc gatatactat gccgatgatt aattgtcaac aggctgcagg tcgaaaggcc
     9481 cggagatgag gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc
     9541 cgggcctccg gaggaccttc gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc
     9601 cccggaccca ccccttccca gcctctgagc ccagaaagcg aaggagcaaa gctgctattg
     9661 gccgctgccc caaaggccta cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag
     9721 actagtgaga cgtgctactt ccatttgtca cgtcctgcac gacgcgagct gcggggcggg
     9781 ggggaacttc ctgactaggg gaggagtaga aggtggcgcg aaggggccac caaagaacgg
     9841 agccggttgg cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc cacttgtgta
     9901 gcgccaagtg cccagcgggg ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc
     9961 ctcccctacc cggtagaatt gacctgcagg ggccctcgat agcttggctg gacgtaaact
    10021 cctcttcaga cctgaagttc ctataccttt tgaagaatag gaacttccgg ttgccgaccc
    10081 gacctgaggg ccagaagcag tgttacacac ttcctggaag cgctaaggaa atccttagcg
    10141 tcaagctgtt ttgtttttaa gttgccttgg catataattt atggtaattt attttgtctg
    10201 ccacttgaac agttgagggg ctacacgaga tttttgtttt aaatgccttg agggacttgc
    10261 tccccacaat cactgtccag ttttagccag ttttaaaata tctcactgac ttattccatt
    10321 tcttgaaagt gtattgatct agagaaattt tgtcatgcgt gttttctcaa tcttaaataa
    10381 atcctggaat ctttttaaaa tctctcgcat cgatttttat caaattaaag atcaacagta
    10441 agtattatac aattagaagt gtatacttaa ttgtaaaatt ttcatttttc ttacaatggt
    10501 aagaaaatgc atctaagaaa ccctaaacac aaacagcggg gtgttcaaat gtgatataat
    10561 tgttctgaga aaaattctca aaagatgaac ttgttatgta cattccaaat attggtttct
    10621 aaaaatgata ctgtaaatgt ttttaattct gtacacttta atgaggctgt tcaaaaattt
    10681 cggattttca gtcatctcga cacctgccaa aagagctttt cagatataac tctttcctgt
    10741 ttgtcttttc ttccaaaagt cttgatccca cttcccacaa aatttgcctt taattcctac
    10801 aattttagag tcagagaaac taatcaaaat aaagtttgtg ttgtgactgt tagtgaactt
    10861 tagtatgtaa tttcaaccgt gcatcttgct atagctagat tgtgtccctt cattttacag
    10921 ttttcaggct caagattctc ctagagtgat ttgtgtgtgg tgtgctttac gcaggtgaag
    10981 acacaaacca gcatccgtcc tattgtgaag aaacaggtac aagactagcc cctgcaaatg
    11041 ctgtgctgag tgctgcggtc cacatggcca tcataggaac tgctggtttg tgggcttttc
    11101 ctgtgccagg tgtttcctta gtgtcattct gcacagcttc ggtctgagca agcctgcctc
    11161 ccagttgagc gctattctct cattttccag tccccataca ggctggggac tgaagccaca
    11221 gcttcatcaa gggagacaag agaaccacct ttgagctgca aacccagtcc agatgctaac
    11281 tcctaatgta tttacagagt ctctttgtgt agggggcata ggtgggcact agaattcaca
    11341 ttctggtcaa catgtaaggt atttagatga tggaaaactt gaaggcgatg gatttgaagg
    11401 gttccatgga aatgtcagtg taacatttta aaggggggat tcctgattac taggcacaga
    11461 agggtagaca ttttcagtga caaaaggttg agcctacttt gttggaaatt tctagtgact
    11521 ataatcattt taaaatttct atttaaaaat ttaaagttca tcccagggag tttatctaac
    11581 ctatttcctt ttcctttctc cctcctagtc cctgacacta ggcaggaaaa ccagagtctc
    11641 tacaacctgt ctcattttat tattactttt tgttgttgtt gttgttgttg ttttctagct
    11701 gtcctggaac tcactttgta gaccaggctg gcttcaaacc cacagagatc tgcatacctc
    11761 tgcctcctaa gtactgtgac taaaggcatg taccaagaca tcaggcttcc aggctgatgt
    11821 ttatttagtg ctcttccctg ctctacactg tttctttcat cccagagagg ttgttctgct
    11881 tgagcataag ttcatttaca gggatttcca agacgttctc agtagactat gtaatgagca
    11941 gaggatttgt ccaggctcac cttcaaggcc acatggctag ttgaaatttc tctagacatt
    12001 ttattgtgtg aatccaaaga aacaactgct ggaaatttaa ctcttaaaat aaagaaggta
    12061 agtagggaaa ggggaaagag agaaagaatc tctaatactt atggcaaagt ccaaaatcag
    12121 attacattac attttttttt ttttagagtg agcaattttt attgaaaccc tcaaagatta
    12181 aagaggacca aaagatgagt tcggtaccat aacaaaaaaa atctcaccta cattacattt
    12241 ttaaccactc ctaaggtgtg aataggaaga caagacagct aaaattattg aggcaatgtt
    12301 actatacaga tggaggtctc ttcatcctca cagacaactc ttgtaggtag agactttggg
    12361 ctagttcctt tcctcaagca ctcaggtaaa gtgatcaaag aatctgctaa tggttgtttt
    12421 gtcctttgat atcatctcat ttttctttca cccttgtatg gagaaaaaac agccaataaa
    12481 ggacaccatg ctccctactg cgtctttctg agatccttta gaaatagata tgagcatcta
    12541 gggcaggttt ctgttgggtt ctaagcagct ttgtatgttt gcatatttgc cttctcctta
    12601 tgattggctg agaaaaacag gaattcttct acaggaatcc tagtaacaca catacaaagc
    12661 acacactctt taattctggc attatgtgac tctttccagc tatcctcggg ttacagaagc
    12721 ctcggtgttg gtcagcaaat aacacagaca gttcaagcat ccaaaacatg acaggcatca
    12781 gatatttgaa atcaaagaac tatttcttcg aggaattctt tgttatgagt cacatgattc
    12841 acatgattta actaacagat actattctct tgaatttaaa gggagcctat aatgtatggt
    12901 gagatgaaga tgaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    12961 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    13021 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    13081 aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta
    13141 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    13201 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
    13261 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag
    13321 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    13381 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
    13441 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    13501 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    13561 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    13621 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    13681 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    13741 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    13801 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    13861 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    13921 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    13981 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    14041 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    14101 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    14161 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    14221 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    14281 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    14341 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    14401 aatctaaagt atatatgagt aaacttggtc tgacag
//
