LOCUS       sequence-30573-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Hosokawa N, Hara T, Kaizuka T, Kishi C, Takamura A, Miura Y, Iemura 
            S, Natsume T, Takehana K, Yamada N, Guan JL, Oshiro N, Mizushima N
  TITLE     Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 
            complex required for autophagy.
  JOURNAL   Mol Biol Cell. 2009 Apr . 20(7):1981-91.
  PUBMED    19211835
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2009 Apr . 20(7):1981-91."
COMMENT     Sequence Label: pME18s-HA-hFIP200
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             55..323
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          353..449
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             502..528
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             532..558
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             562..588
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcctcga tcgaggggct
       61 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
      121 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
      181 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
      241 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
      301 tctgttctgc gccgttacag atccaagctc tgaaaaacca gaaagttaac tggtaagttt
      361 agtctttttg tcttttattt caggtcccgg atccggtggt ggtgcaaatc aaagaactgc
      421 tcctcagtgg atgttgcctt tacttctagg cctgtacgga agtgttactt ctgctctaaa
      481 agctgcggaa ttgccatggc atacccttat gacgtaccag attacgcggg atatccctac
      541 gatgtccccg attacgcgcg ttatccatat gacgtcccgg actatgccga attcatgaag
      601 ttatatgtat ttctggttaa cactggaact actctaacat ttgacactga acttacagtg
      661 caaactgtgg cagaccttaa gcatgccatt caaagcaaat acaagattgc tattcaacac
      721 caggtgctgg tggtcaatgg aggagaatgc atggctgcag atcgaagagt gtgtacctac
      781 agtgctggga cggatacaaa tccaattttt ctttttaaca aagaaatgat cttatgtgat
      841 cgtccacctg ctattcctaa aacta
//
