LOCUS       sequence-305733-        9431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-Cas9 library validation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Arnan C, Ullrich S, Pulido-Quetglas C, Nurtdinov R, Esteban A, 
            Blanco-Fernandez J, Aparicio-Prat E, Johnson R, Perez-Lluch S, Guigo
            R
  TITLE     Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and 
            lncRNAs involved in transdifferentiation of human B-cells to 
            macrophages.
  JOURNAL   BMC Genomics. 2022 May 26;23(1):402. doi: 
            10.1186/s12864-022-08612-7.
  PUBMED    35619054
REFERENCE   2  (bases 1 to 9431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2022-05-26"; volume: "23(1):402. doi"; pages: " 
            10.1186/s12864-022-08612-7"
COMMENT     Sequence Label: pDECKO_mCherry_FURIN
FEATURES             Location/Qualifiers
     source          1..9431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    97..118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(133..138,139..156,157..163)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    171..187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     176..198
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     195..211
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     195..211
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     230..250
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        232..250
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        278..504
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             505..685
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    732..857
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1350..1583
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1768..1812
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1990..2238
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1990..2010
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2161..2180
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2260..2335
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2342..2556
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     2477..2496
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        2585..2660
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2716..2833
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2972..4150
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3203..4141
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4098..4118
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4163..4759
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4163..4182)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4659..4679
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4769..4822
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4829..4838
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4835..5545
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4980..4998)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5248..5267
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5561..6149
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5614..5634)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6032..6043)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6221..6454
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6532..6653
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6569..6588)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6623..6642
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6693..6828
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6848..6867)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6849..6867)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6877..6894)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6877..6893)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6886..6908)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7035..7490
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7122..7141)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7332..7353
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7516..7620
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7621..7689,7690..8481)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7839..7858)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8652..9240
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9141..9160
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9394..9411
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
       61 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      121 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      181 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
      241 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
      301 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
      361 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
      421 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
      481 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
      541 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
      601 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
      661 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
      721 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
      781 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
      841 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
      901 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
      961 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1021 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1081 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1141 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1201 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     1261 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     1321 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     1381 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     1441 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     1501 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     1561 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     1621 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     1681 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     1741 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     1801 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     1861 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     1921 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     1981 ggacccagag agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     2041 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg
     2101 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     2161 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     2221 tggaaaggac gaaacaccgg aggccctggt tgctatgggg ttttagagct agaaatagca
     2281 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     2341 tgaacgctga cgtcatcaac ccgctccaag gaatcgcggg cccagtgtca ctaggcggga
     2401 acacccagcg cgcgtgcgcc ctggcaggaa gatggctgtg agggacaggg gagtggcgcc
     2461 ctgcaatatt tgcatgtcgc tatgtgttct gggaaatcac cataaacgtg aaatgtcttt
     2521 ggatttggga gtcttataag ttctgtatga gaccactctt tcccagaagg tcttcaccaa
     2581 cacggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
     2641 aagtggcacc gagtcggtgc ttttttaagc ttggcgtaac tagatcttga gacaaatggc
     2701 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     2761 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     2821 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga
     2881 gggggcccgg gtgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg
     2941 gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg
     3001 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgat ccggtgccta
     3061 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     3121 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     3181 cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt
     3241 tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct
     3301 tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc
     3361 cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat
     3421 ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt
     3481 ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga
     3541 tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca
     3601 gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta
     3661 gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc
     3721 ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc
     3781 cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga
     3841 gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac
     3901 ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc
     3961 tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac
     4021 tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt
     4081 tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca
     4141 ggtgtcgtga cgtacggcca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     4201 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     4261 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     4321 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt
     4381 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     4441 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     4501 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggag tctcgcccga
     4561 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     4621 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     4681 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     4741 gacccgcaag cccggtgccg gatcgggaga gggcagagga agtctgctaa catgcggtga
     4801 cgtcgaggag aatcctggcc caccggtcgc caccatggtg agcaagggcg aggaggataa
     4861 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     4921 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     4981 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     5041 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     5101 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     5161 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     5221 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     5281 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     5341 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     5401 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     5461 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     5521 cggcatggac gagctgtaca agtaaacgcg ttaagtcgac aatcaacctc tggattacaa
     5581 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     5641 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     5701 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     5761 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     5821 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     5881 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     5941 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     6001 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6061 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     6121 tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca
     6181 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     6241 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     6301 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6361 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6421 agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt
     6481 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     6541 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6601 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6661 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     6721 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6781 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     6841 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     6901 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     6961 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     7021 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     7081 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     7141 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     7201 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7261 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7321 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7381 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     7441 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     7501 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     7561 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     7621 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     7681 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7741 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     7801 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     7861 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     7921 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     7981 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     8041 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     8101 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     8161 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     8221 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     8281 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     8341 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     8401 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     8461 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     8521 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     8581 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     8641 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     8701 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8761 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     8821 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     8881 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     8941 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     9001 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     9061 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     9121 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9181 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     9241 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     9301 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     9361 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     9421 gagcgcccaa t
//
