LOCUS       sequence-30574-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Hosokawa N, Hara T, Kaizuka T, Kishi C, Takamura A, Miura Y, Iemura 
            S, Natsume T, Takehana K, Yamada N, Guan JL, Oshiro N, Mizushima N
  TITLE     Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 
            complex required for autophagy.
  JOURNAL   Mol Biol Cell. 2009 Apr . 20(7):1981-91.
  PUBMED    19211835
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2009 Apr . 20(7):1981-91."
COMMENT     Sequence Label: p3xFLAG-CMV14-mULK1
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(180..245)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cttgcccctt gctccatacc acccccctcc accccataat attatagaag gacacctagt
       61 cagacaaaat gatgcaactt aattttatta ggacaaggct ggtgggcact ggagtggcaa
      121 cttccagggc caggagaggc actggggagg ggtcacaggg atgccacccg ggatcactac
      181 ttgtcatcgt catccttgta gtcgatgtca tgatctttat aatcaccgtc atggtctttg
      241 tagtcagccc gggatcctct agagtcgact ggtaccgata tcagatctat cgatgaattc
      301 gcggcataga caccactcag cagggccgag agtctcctct caatgcacag cttgcatttg
      361 gcaatgttct caatgtctgc ctggtccgtg agagtgtgct gcaacccctc cagcaatagc
      421 agggctttgt gatatctcgg tacacagccc tctcggtgct ggaacatctc atcaagggca
      481 gctgattgta ccatttgcac agcatggctg aggatgagcc gctctgcagt gacaccatgg
      541 atcccgtcca gcagccgttg tttgtccaga aagaagcgct gaagtcgcaa gctgaggccc
      601 tggcaggata ccacgctggc cttgtacagc tcatttagtc tgcgtaccac ctgcttcaca
      661 gtagatgaaa ggcagagttt gccagctcga atctggtcaa tggcagtctg taggcctgag
      721 gacagcagct cagccacctt caagtacaga accagttgct ctgcaaagcc ccactctcgg
      781 ctcaactgac tgatctggtc agccaccaca ctttcctgga gctggtactc a
//
