LOCUS       sequence-305742-        9682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9682)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 9682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP26
FEATURES             Location/Qualifiers
     source          1..9682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(27..526)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(75..94)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     468..491
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    545..569
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3312..3336)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3352..3378)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3391..3414)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      3414..3423
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3496..3520)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3530..3548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3551..3569
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(3571..3774)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3600..3620)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3775..4154
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(4169..4585)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(4185..4201)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(4217..4239)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    4652..4993
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             5057..5573
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(5773..5792)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5920..5942)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6136..6155)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6255..6277
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6315..6333)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6401..6505
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6506..6574,6575..7366)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6724..6743)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7537..8125
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8026..8045
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8279..8296
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8413..8434
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8449..8454,8455..8472,8473..8479)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8487..8503
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(join(9089..9682,1..6))
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9172..9192)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     join(9669..9682,1..6)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ggtcatggta agcttgggct gcaggtcgaa aggcccggag atgaggaaga ggagaacagc
       61 gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga ccttcgggcg
      121 cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct tcccagcctc
      181 tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag gcctacccgc
      241 ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc tacttccatt
      301 tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac taggggagga
      361 gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct accggtggat
      421 gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag cggggctgct
      481 aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta gaattcgtta
      541 tcgaaccact ttgtacaaga aagttgggta ttattccttc tgaagggtct cctctacctc
      601 cttgctatta agctgggcat tctcttctct tttcaacatc tcttcaaaga tctcattatg
      661 catgtaaaag aagatgtacc cagtgcaacg cctatcctcc tgcatctggg cctcctggat
      721 acctaacacc cgcatatcat cgtaagtgaa ccagatctgt ttctcaaagt cataggcatc
      781 acagatataa tggcctgact ttagagtctt cccaagatgg ctgacaacac taatgagccg
      841 gtaggtatga tctcccttat catcatttct ttttgtttcc ttggctttag attttatctt
      901 atctaaaatg tctttgtttc ttggattctt attggaaccc agtgcaagta gggaattcct
      961 gttagacttc tgtagattta attttgtagg tcttaggagg ttctttgtgt gcccctgggt
     1021 gcctggcttt ggaaagcttt gaggcagtgc ctgctgaggg gcttgttcac agattctcat
     1081 accgtcacac tgctgagtct gttcagacac tttttggaat ctttctggaa ttctgatatt
     1141 tttatcttca tacaaatctt tagtaggatt gataatccta tcaaaagcta caaacttact
     1201 ggttttcaca tttttctttc gttttggatt ttcgggcact gtttgaaagt caacttttga
     1261 gagaggtgtg cctgggctgc tggcaggttt acctccagct ttgtggaact gacaaagtga
     1321 ggtatcttcc agatacgtca ttaagtgagc taacggttct ttttcaatga atgctcgatc
     1381 tcctgagtat acagattcta gctcatttgg tttagaattt tttccaaggt acttttgctg
     1441 ctgtcttctg cttgcccctt taaagactgt ctggcctttt ttttgttcag actccttatc
     1501 tgatccaatg tgtggagcca ggatatcttt ggattccttt gttgcaggcc atgatacact
     1561 gatgtttcca gaagtcatct ttcgaataac ttttaataat tggaaatctg taatttctcc
     1621 atcctcactc aagggaagag gtggtctggt gccttcattg caatgagaag acacctttaa
     1681 atatttggaa atgatgactt cctggtcatt cttctttaat gcacaaaact cattcaagct
     1741 atagcgtttg aggtgaacaa taaggattct aggtagccta ctgaatgagt gcactccaac
     1801 ggaagtcttg tgctcacatt ttgcacattt atactcaagc tcttctgctc caaaaaaaag
     1861 atcaaaagta gactgaatag atgaaggatg tgcttttatt ctttggggaa ggttgatgga
     1921 gaggtaatta ttcagttctg tcttgagaat aacctgacca caagctttac aagcaatgga
     1981 gtgcaacaac tctaactcaa aattagtaat gacagggcaa gaaaacccac tggtgtcagg
     2041 atcatcagca aaaacctgtt taggaaaatt atcttcccca aattcacttt taggcttcca
     2101 aattgtgttg agtttttcca tgttatcttt cagttgatct aaacagtgag ctaaaaactc
     2161 atgagcatcg ttctgtgcat tgccatggaa tatctctgca gctgctgaaa tggccttttt
     2221 aagattcaag agtaacatct ccttgatttc tatattatag gtatctttaa aaaaaagtag
     2281 ccgtgccaag cacatggtaa gagcattaag gggaatttta ccccatggga aactctgatt
     2341 aagtaaatca tcagcaaacg atgggattga aagtagagac tgtaacactg cattcatata
     2401 acaggtgttt cccaaattgg ggaggccgtg gcatattttc tctggaaata attcaaaaaa
     2461 tagttttaat ttatcccact ttgtgtaacc gtcactatac ccttgttgta acagaaatac
     2521 caaaaccatt ttctcagtga gagcttggag aagaccaatg tcatctaggt aaggatttcc
     2581 agtggcgttc atgatgcatg aagattcaca ttccaatttc ttattctctt ctaactcttt
     2641 taacttcaat tgtttctctc gattatagct tacacacctc gaacaatctg ccttggattt
     2701 cttgtattct acagaattat tttctttcaa gaattcttca ttcatctctg agctagatga
     2761 gagcattctt ttcctcttct tgtgctgatt ttctgataac tctccgcaag taagtgtcaa
     2821 ttttgatgta agcaaaggca tcctctgaag gacacctgtc ccacttcctt ttgctatctc
     2881 aaaagatttg ctacttgatt tctcatcaac tttgtggaat gaagttttgt tgatttcctt
     2941 ctgtgttgtg ctagaaaaga cactcccacc cttaccaggt ctcacaggtg gctgaacctc
     3001 gttttgatga actctgtcca agaatatctt caattgttca gcatctgtgg aggataatcc
     3061 ttcaataaac aagccattat tattttgtaa agttaaatgc aggtgatttt ggtttcctct
     3121 ataggattta aggactacat tttgaatatt atcacttagc cgaaaagtgc tatattttcc
     3181 acttttgaaa tacagcacca gtctatcttt cttctttctt tccactgctt caatgaatgc
     3241 ttcttttgac ttagatatcc cagtcttgca gttccctatt tggacaaaac cacgtaggaa
     3301 tagggcagcc atgccaactt ttttgtacaa acttgtttga taacctcgag cagcgtaatc
     3361 tggaacatcg tatgggtatc ctccatcgag cttgtcatcg tcatccttgt agtccatggt
     3421 ggcagatccc cggaggctgg atcggtcccg gtgtcttcta tggaggtcaa aacagcgtgg
     3481 atggcgtctc caggcgatct gacggttcac taaacgagct cgtcgacgat ctctatcact
     3541 gatagggaga tctctatcac tgatagggag agctctgctt atatagacct cccaccgtac
     3601 acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg
     3661 ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc
     3721 ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt
     3781 aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta
     3841 ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg
     3901 catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg
     3961 acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa
     4021 tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg
     4081 gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt
     4141 caataatcaa tgtcaaggat ctaattccgg cgcctagaga aggagtgagg gctggataaa
     4201 gggaggatcg aggcggggtc gaacgaggag gttcaagggg gagagacggg gcggatggag
     4261 gaagaggagg cggaggctta gggtgtacaa agggcttgac ccagggaggg gggtcaaaag
     4321 ccaaggcttc ccaggtcacg atgtagggga cctggtctgg gtgtccatgc gggccaggtg
     4381 aaaagacctt gatcttaacc tgggtgatga ggtctcggtt aaaggtgccg tctcgcggcc
     4441 atccgacgtt aaaggttggc cattctgcag agcagaaggt aacccaacgt ctcttcttga
     4501 catctaccga ctggttgtga gcgatccgct cgacatcttt ccagtgacct aaggtcaaac
     4561 ttaagggagt ggtaacagtc tggccctaat tttcagacaa atacagaaac acagtcagac
     4621 agagacaaca cagaacgatg ctgcagcgct gcagcagaca agacgcgcgg cttcggttcc
     4681 aaaccgaaag caaaaattca gacggaggcg ggaactgttt taggttctcg tctcctacca
     4741 gaaccacata tcctgacggg gtcggattcc acatcgactc ccttcctcag gtcgggccac
     4801 aaaaacggcc cccaaagtcc ctgggacgtc tcccagggtt gcggccgggt gttcagaact
     4861 cgtcagttcc accacgggtc cgccagatac agagctagtt agctaactag tacagacgca
     4921 ggcgcaaaca ttagatgccg gcacaaacac gcacaaagac agagacagac acgaaacgac
     4981 cgctggccag cttacctccc ggcggggggg tcggtggtcc ctgggcaggg gtctccaaat
     5041 ctcggtggaa cctccaaatg aaagaccccc gaggtgggca gtcaatcaat ctgaggagac
     5101 cctcccaagg atcagcgagt ccacgattcg gatgcaaaca gcaagaggct ttattgggaa
     5161 tacgggtacc cgggcgacgc agtctatcgg aggactggcg cgccgagtga ggggttgtgg
     5221 gctcttttat tgagctcggg gagcagaagc gcgcgaacag aagcgagaag cgaactgatt
     5281 ggttagttca aataaggcac agggtcattt caggtccttg gggcaccctg gaaacatctg
     5341 atggttctct agaaactgct gagggcggga ccgcatctgg ggaccatctg ttcttggccc
     5401 tgagccgggg caggaactgc ttaccacaga tatcctgttt ggcccatatt ctgctgtctc
     5461 tctgttccta accttgatct gaacttctct attctcagtt atgtattttc catgccttgc
     5521 aaaatggcgt tacttaagct agcttgccaa acctacaggt ggggtctttc attcccccct
     5581 ttttctggag actaaataaa atcttttatt ttatctatgg ctcgtactct ataggcttca
     5641 gctggtgata ttgttgagtc aaaactagag cctggaccac tgatatcctg tctttaacaa
     5701 attggactaa tcgcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
     5761 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
     5821 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
     5881 tgggcgccat ctccttgcat gcaccattcc ttgcgccgtc gttttacaac gtcgtgactg
     5941 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     6001 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     6061 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     6121 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     6181 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     6241 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     6301 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     6361 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6421 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6481 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6541 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6601 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6661 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6721 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6781 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6841 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6901 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6961 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     7021 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7081 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     7141 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     7201 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     7261 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     7321 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     7381 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7441 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7501 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7561 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7621 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7681 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7741 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7801 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7861 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7921 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7981 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8041 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8101 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8161 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8221 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8281 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     8341 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     8401 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     8461 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca attctcccca
     8521 gctctttctg cgctgtccat aatgaactat ttcaggagta ttggagtatg tggattcaaa
     8581 acagtggcta attgagtcag gattacttaa tattcgactc ctaccctcag aatgttgttc
     8641 tccaacttca agaaactgtt acccatgaaa gacccccgct gacgggtagt caatcactca
     8701 gaggagaccc tcccaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta
     8761 ttggatacac gggtacccgg gcgacgcagt ctatcggagg actggcgcgc cgagtgaggg
     8821 gttgtgggct cttttattga gctcggggag cagaagcgcg cgaacagaag cgagaagcga
     8881 actgattggt tagttcaaat aaggcacagg gtcatttcag gtccttgggg caccctggaa
     8941 acatctgatg gttctctagc ttgccaaacc tacaggtggg gtctttcatt cccccctttt
     9001 tctggagact aaataaaatc ttttatttta tcgatgcatg gggtcgtgcg ctcctttcgg
     9061 tcgggcgctg cgggtcgtgg ggcgggcgtc aggcaccggg cttgcgggtc atgcaccagg
     9121 tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc cgctcgtaga
     9181 aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg cgctcggccg
     9241 cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg tcgggcgaga
     9301 cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc aggaggcctt
     9361 ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc gggccgatct
     9421 cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc accgcggcgc
     9481 cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg aagagttctt
     9541 gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc gtggcggggt
     9601 agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg cgggtggcga
     9661 ggcgcaccgt gggcttgtac tc
//
