LOCUS       sequence-305757-        4327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Reynet C, Kahn CR
  TITLE     Rad: a member of the Ras family overexpressed in muscle of type II 
            diabetic humans.
  JOURNAL   Science. 1993 Nov 26. 262(5138):1441-4.
  PUBMED    8248782
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1993 Nov 26. 262(5138):1441-4."
COMMENT     Sequence Label: pBS Rad
FEATURES             Location/Qualifiers
     source          1..4327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     191..207
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     191..207
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1609..1625)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1610..1626)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(1650..1669)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1651..1669)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1676..1693)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1676..1692)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1685..1707)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(1834..2289)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1971..1992)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2183..2202
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2316..2420
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2421..2489,2490..3281)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2639..2658)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3452..4040
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3941..3960
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4194..4211
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag ctcgaaatta accctcacta aagggaacaa aagctggagc tccaccgcgg
      181 tggcggccgc tctagaacta gtggatccta gtcctgagcg tgtctgtgtg cgagtggacg
      241 gtcccggacg cgatgaccct gaacggcggc ggcagcggag cgggcgggag ccgcggtggg
      301 ggccaggagc gcgagcgccg tcggggcagc acaccctggg gccccgcccc gccgctgcac
      361 cgccgcagca tgccggtgga cgagcgcgac ctgcaggcgg cgctgacccc gggtgccctg
      421 acggcggccg cggccgggac ggggacccag ggtcccaggc tggactggcc cgaggactcc
      481 gaggactcgc tcagctcagg gggcagcgac tcagacgaga gcgtttacaa ggtgctgctg
      541 ctgggggcgc ccggcgtggg caagagcgcc ctggcgcgca tcttcggcgg tgtggaggac
      601 gggcctgaag cagaggcagc agggcacacc tatgatcgct ccattgtagt ggacggagaa
      661 gaggcatcac tcatggtcta cgacatttgg gagcaggacg ggggccgctg gttgcccggc
      721 cactgcatgg ccatggggga tgcctatgtc attgtgtact cagtgacgga caagggcagc
      781 ttcgagaagg cctcagaact gcgggtccag ctgcggcgtg cacggcaaac agatgatgtg
      841 cccatcatcc tcgtgggcaa caagagcgac ctggtgcgct ctcgtgaggt ctcggtggat
      901 gagggccggg cctgcgcggt ggtctttgac tgcaagttca ttgagacatc agcggcattg
      961 caccacaatg tccaggcgct gtttgaaggt gtcgtgcgcc agatacgcct gcgcagggac
     1021 agcaaagaag ccaacgcacg acggcaagca ggcacccgga ggcgagagag ccttggcaaa
     1081 aaggcgaagc gcttcttggg ccgcatcgta gctcgtaaca gccgcaagat ggcctttcgc
     1141 gccaaatcca agtcctgcca cgacctctcg gttctctagg tcccacccgc tcccactatg
     1201 gtgggagacg aacggaaggg ttggtgggct ggcccagcca actgccccgg gtgcctcaga
     1261 gcaggctcag actctgggtc cctcggagct gccagccggg cacccccaac ctcatggtca
     1321 tggacagata gacagtgctg ccctgcgaag tggctctcag gggccagtga gggctgggcc
     1381 cacagagatg catgcgcagg ctcatatgcg tcccaagcag ccgcagcgca gccgccgggc
     1441 aggcctgcgt gccgggagag gactctgcct tttttcacag cccgggtgtg cctgccctgg
     1501 agggaggctc ttcagtgcgg tagctacttg tttacatgca gatttttgta ataaaggcta
     1561 tttcctgata aaaaaaaaaa aaaaaaagga attcgatatc aagcttatcg ataccgtcga
     1621 cctcgagggg gggcccggta cccaattcgc cctatagtga gtcgtattac aattcactgg
     1681 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     1741 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     1801 cccaacagtt gcgcagcctg aatggcgaat ggcaaattgt aagcgttaat attttgttaa
     1861 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     1921 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     1981 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     2041 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     2101 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     2161 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     2221 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     2281 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     2341 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     2401 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     2461 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     2521 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     2581 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2641 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2701 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2761 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2821 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2881 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2941 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     3001 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     3061 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     3121 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     3181 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     3241 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     3301 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     3361 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3421 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3481 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3541 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3601 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3661 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3721 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3781 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3841 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3901 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3961 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4021 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4081 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4141 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     4201 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     4261 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     4321 acgcaat
//
