LOCUS       sequence-305776-        5562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Liu X, Verma A, Garcia G Jr, Ramage H, Lucas A, Myers RL, Michaelson
            JJ, Coryell W, Kumar A, Charney AW, Kazanietz MG, Rader DJ, Ritchie 
            MD, Berrettini WH, Schultz DC, Cherry S, Damoiseaux R, Arumugaswami 
            V, Klein PS
  TITLE     Targeting the coronavirus nucleocapsid protein through GSK-3 
            inhibition.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Oct 19;118(42). pii: 2113401118. doi:
            10.1073/pnas.2113401118. Epub 2021 Sep 30.
  PUBMED    34593624
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-10-19"; volume: "118(42). pii: 
            2113401118. doi"; pages: " 10.1073/pnas.2113401118. Epub 2021 Sep 
            30"
COMMENT     Sequence Label: SARS-CoV2 Nucleocapsid
FEATURES             Location/Qualifiers
     source          1..5562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..978
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1013..1031
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1013..1030
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1081..1110
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1120..1149
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1159..1188
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1198..1227
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1237..1266
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    2592..2726
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2616..2635)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2670..2689
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2839..2859)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2839..2857)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2878..2894)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2878..2894)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2891..2913)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2902..2918
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2926..2932,2933..2950,2951..2956))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2971..2992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3109..3126)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3280..3868)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3360..3379)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4039..4830,4831..4899))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4662..4681
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4900..5004)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5030..5485)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5167..5188)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5379..5398
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gccaattcaa tatggcgtat atggactcat gccaattcaa tatggtggat ctggacctgt
       61 gccaattcaa tatggcgtat atggactcgt gccaattcaa tatggtggat ctggacccca
      121 gccaattcaa tatggcggac ttggcaccat gccaattcaa tatggcggac ctggcactgt
      181 gccaactggg gaggggtcta cttggcacgg tgccaagttt gaggaggggt cttggccctg
      241 tgccaagtcc gccatattga attggcatgg tgccaataat ggcggccata ttggctatat
      301 gccaggatca atatataggc aatatccaat atggccctat gccaatatgg ctattggcca
      361 ggttcaatac tatgtattgg ccctatgcca tatagtattc catatatggg ttttcctatt
      421 gacgtagata gcccctccca atgggcggtc ccatatacca tatatggggc ttcctaatac
      481 cgcccatagc cactccccca ttgacgtcaa tggtctctat atatggtctt tcctattgac
      541 gtcatatggg cggtcctatt gacgtatatg gcgcctcccc cattgacgtc aattacggta
      601 aatggcccgc ctggctcaat gcccattgac gtcaatagga ccacccacca ttgacgtcaa
      661 tgggatggct cattgcccat tcatatccgt tctcacgccc cctattgacg tcaatgacgg
      721 taaatggccc acttggcagt acatcaatat ctattaatag taacttggca agtacattac
      781 tattggaagt acgccagggt acattggcag tactcccatt gacgtcaatg gcggtaaatg
      841 gcccgcgatg gctgccaagt acatccccat tgacgtcaat ggggaggggc aatgacgcaa
      901 atgggcgttc cattgacgta aatgggcggt aggcgtgcct aatgggaggt ctatataagc
      961 aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt
     1021 gacactatag aatacaagct acttgttctt tttgcaggat cccatcgatt taaagctatg
     1081 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa
     1141 gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag
     1201 caaaagctca tttctgaaga ggacttgaat gaaatggagc aaaagctcat ttctgaagag
     1261 gacttgaatg aaatggagag cttgggcgac ctcaccatgg agatgtctga taatggaccc
     1321 caaaatcagc gaaatgcacc ccgcattacg tttggtggac cctcagattc aactggcagt
     1381 aaccagaatg gagaacgcag tggggcgcga tcaaaacaac gtcggcccca aggtttaccc
     1441 aataatactg cgtcttggtt caccgctctc actcaacatg gcaaggaaga ccttaaattc
     1501 cctcgaggac aaggcgttcc aattaacacc aatagcagtc cagatgacca aattggctac
     1561 taccgaagag ctaccagacg aattcgtggt ggtgacggta aaatgaaaga tctcagtcca
     1621 agatggtatt tctactacct aggaactggg ccagaagctg gacttcccta tggtgctaac
     1681 aaagacggca tcatatgggt tgcaactgag ggagccttga atacaccaaa agatcacatt
     1741 ggcacccgca atcctgctaa caatgctgca atcgtgctac aacttcctca aggaacaaca
     1801 ttgccaaaag gcttctacgc agaagggagc agaggcggca gtcaagcctc ttctcgttcc
     1861 tcatcacgta gtcgcaacag ttcaagaaat tcaactccag gcagcagtag gggaacttct
     1921 cctgctagaa tggctggcaa tggcggtgat gctgctcttg ctttgctgct gcttgacaga
     1981 ttgaaccagc ttgagagcaa aatgtctggt aaaggccaac aacaacaagg ccaaactgtc
     2041 actaagaaat ctgctgctga ggcttctaag aagcctcggc aaaaacgtac tgccactaaa
     2101 gcatacaatg taacacaagc tttcggcaga cgtggtccag aacaaaccca aggaaatttt
     2161 ggggaccagg aactaatcag acaaggaact gattacaaac attggccgca aattgcacaa
     2221 tttgccccca gcgcttcagc gttcttcgga atgtcgcgca ttggcatgga agtcacacct
     2281 tcgggaacgt ggttgaccta cacaggtgcc atcaaattgg atgacaaaga tccaaatttc
     2341 aaagatcaag tcattttgct gaataagcat attgacgcat acaaaacatt cccaccaaca
     2401 gagcctaaaa aggacaaaaa gaagaaggct gatgaaactc aagccttacc gcagagacag
     2461 aagaaacagc aaactgtgac tcttcttcct gctgcagatt tggatgattt ctccaaacaa
     2521 ttgcaacaat ccatgagcag tgctgactca actcaggcct aatctagaac tatagtgagt
     2581 cgtattacgt agatccagac atgataagat acattgatga gtttggacaa accacaacta
     2641 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2701 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2761 ttcaggggga ggtgtgggag gttttttaat tcgcggccgc ggcgccaatg cattgggccc
     2821 ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     2881 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2941 gagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3001 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3061 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3121 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3181 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3241 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3301 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3361 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3421 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3481 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3541 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3601 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3661 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3721 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3781 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3841 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3901 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3961 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4021 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4081 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4141 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     4201 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4261 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     4321 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     4381 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     4441 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     4501 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     4561 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     4621 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     4681 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4741 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4801 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     4861 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4921 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4981 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     5041 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     5101 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     5161 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     5221 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     5281 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     5341 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     5401 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     5461 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
     5521 gggcgatcgg tgcgggcctc ttcgctatta cgccagtcga cc
//
