LOCUS       sequence-305778-        9606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector encoding untagged WT human AMPK alpha 
            1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9606)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 9606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-01-15- 15"; volume: "351"; 
            issue: "6270"; pages: "275-81"
COMMENT     Sequence Label: pLenti.CMV/TO_PRKAA1
FEATURES             Location/Qualifiers
     source          1..9606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2122..2425
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2426..2629
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2580..2600
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2631..2649
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2652..2670
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2711..2735
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4392..4416)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4447..5035
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4500..4520)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4918..4929)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5049..5548
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5084..5107)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5481..5500
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5569..6168
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5569..5588)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6065..6085
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6306..6539
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6611..6732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6648..6667)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6702..6721
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6772..6907
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6927..6946)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6928..6946)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6956..6973)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6956..6972)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6965..6987)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7114..7569
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7201..7220)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7411..7432
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7595..7699
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7700..7768,7769..8560)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7918..7937)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8731..9319
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9220..9239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9473..9490
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgataagct tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2161 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     2221 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2281 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2341 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2401 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     2461 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2521 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2581 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag
     2641 tgatagagat ctccctatca gtgatagaga tcgtcgacta gtccagtgtg gtggaattct
     2701 gcagatatca acaagtttgt acaaaaaagc aggcaccatg gcgacagccg agaagcagaa
     2761 acacgacggg cgggtgaaga tcggccacta cattctgggt gacacgctgg gggtcggcac
     2821 cttcggcaaa gtgaaggttg gcaaacatga attgactggg cataaagtag ctgtgaagat
     2881 actcaatcga cagaagattc ggagccttga tgtggtagga aaaatccgca gagaaattca
     2941 gaacctcaag cttttcaggc atcctcatat aattaaactg taccaggtca tcagtacacc
     3001 atctgatatt ttcatggtga tggaatatgt ctcaggagga gagctatttg attatatctg
     3061 taagaatgga aggctggatg aaaaagaaag tcggcgtctg ttccaacaga tcctttctgg
     3121 tgtggattat tgtcacaggc atatggtggt ccatagagat ttgaaacctg aaaatgtcct
     3181 gcttgatgca cacatgaatg caaagatagc tgattttggt ctttcaaaca tgatgtcaga
     3241 tggtgaattt ttaagaacaa gttgtggctc acccaactat gctgcaccag aagtaatttc
     3301 aggaagattg tatgcaggcc cagaggtaga tatatggagc agtggggtta ttctctatgc
     3361 tttattatgt ggaacccttc catttgatga tgaccatgtg ccaactcttt ttaagaagat
     3421 atgtgatggg atcttctata cccctcaata tttaaatcct tctgtgatta gccttttgaa
     3481 acatatgctg caggtggatc ccatgaagag ggccacaatc aaagatatca gggaacatga
     3541 atggtttaaa caggaccttc caaaatatct ctttcctgag gatccatcat atagttcaac
     3601 catgattgat gatgaagcct taaaagaagt atgtgaaaag tttgagtgct cagaagagga
     3661 agttctcagc tgtctttaca acagaaatca ccaggatcct ttggcagttg cctaccatct
     3721 cataatagat aacaggagaa taatgaatga agccaaagat ttctatttgg cgacaagccc
     3781 acctgattct tttcttgatg atcatcacct gactcggccc catcctgaaa gagtaccatt
     3841 cttggttgct gaaacaccaa gggcacgcca tacccttgat gaattaaatc cacagaaatc
     3901 caaacaccaa ggtgtaagga aagcaaaatg gcatttagga attagaagtc aaagtcgacc
     3961 aaatgatatt atggcagaag tatgtagagc aatcaaacaa ttggattatg aatggaaggt
     4021 tgtaaaccca tattatttgc gtgtacgaag gaagaatcct gtgacaagca cttactccaa
     4081 aatgagtcta cagttatacc aagtggatag tagaacttat ctactggatt tccgtagtat
     4141 tgatgatgaa attacagaag ccaaatcagg gactgctact ccacagagat cgggatcagt
     4201 tagcaactat cgatcttgcc aaaggagtga ttcagatgct gaggctcaag gaaaatcctc
     4261 agaagtttct cttacctcat ctgtgacctc acttgactct tctcctgttg acctaactcc
     4321 aagacctgga agtcacacaa tagaattttt tgagatgtgt gcaaatctaa ttaaaattct
     4381 tgcacaatag aacccagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg
     4441 ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     4501 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     4561 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     4621 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     4681 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     4741 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4801 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     4861 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4921 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4981 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     5041 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
     5101 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
     5161 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc
     5221 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga
     5281 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt
     5341 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct
     5401 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg
     5461 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
     5521 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac
     5581 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     5641 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     5701 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     5761 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     5821 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     5881 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     5941 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6001 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6061 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6121 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac
     6181 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catctcgagg gcccggtacc
     6241 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     6301 gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg cttgtactgg
     6361 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6421 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6481 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6541 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     6601 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     6661 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     6721 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     6781 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6841 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     6901 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     6961 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     7021 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     7081 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     7141 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     7201 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     7261 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     7321 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     7381 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7441 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     7501 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     7561 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     7621 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7681 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     7741 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     7801 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     7861 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     7921 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     7981 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8041 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8101 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8161 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8221 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8281 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8341 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8401 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8461 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8521 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8581 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8641 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8701 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8761 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8821 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     8881 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8941 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9001 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9061 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     9541 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     9601 cgcaat
//
