LOCUS       sequence-305791-        9431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-Cas9 Knock down.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Arnan C, Ullrich S, Pulido-Quetglas C, Nurtdinov R, Esteban A, 
            Blanco-Fernandez J, Aparicio-Prat E, Johnson R, Perez-Lluch S, Guigo
            R
  TITLE     Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and 
            lncRNAs involved in transdifferentiation of human B-cells to 
            macrophages.
  JOURNAL   BMC Genomics. 2022 May 26;23(1):402. doi: 
            10.1186/s12864-022-08612-7.
  PUBMED    35619054
REFERENCE   2  (bases 1 to 9431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2022-05-26"; volume: "23(1):402. doi"; pages: " 
            10.1186/s12864-022-08612-7"
COMMENT     Sequence Label: pDECKO_mCherry_TREML2
FEATURES             Location/Qualifiers
     source          1..9431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     12..29
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    146..167
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(182..187,188..205,206..212)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    220..236
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     225..247
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     244..260
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     244..260
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     279..299
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        281..299
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        327..553
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             554..734
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    781..906
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1399..1632
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1817..1861
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2039..2287
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2039..2059
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2210..2229
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2309..2384
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2391..2605
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     2526..2545
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        2634..2709
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2765..2882
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3021..4199
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3252..4190
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4147..4167
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4212..4808
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4212..4231)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4708..4728
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4818..4871
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4878..4887
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4884..5594
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5029..5047)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5297..5316
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5610..6198
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5663..5683)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6081..6092)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6270..6503
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6581..6702
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6618..6637)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6672..6691
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6742..6877
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6897..6916)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6898..6916)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6926..6943)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6926..6942)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6935..6957)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7084..7539
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7171..7190)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7381..7402
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7565..7669
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7670..7738,7739..8530)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7888..7907)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8701..9289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9190..9209
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
       61 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
      121 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
      181 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
      241 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
      301 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
      361 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
      421 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
      481 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
      541 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
      601 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
      661 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
      721 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
      781 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
      841 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
      901 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
      961 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     1021 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     1081 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     1141 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     1201 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     1261 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     1321 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     1381 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     1441 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     1501 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     1561 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     1621 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     1681 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     1741 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     1801 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     1861 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     1921 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     1981 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccagaga
     2041 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     2101 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     2161 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
     2221 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
     2281 aaacaccggg tgaacaccat cacaccaggt tttagagcta gaaatagcaa gttaaaataa
     2341 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt gaacgctgac
     2401 gtcatcaacc cgctccaagg aatcgcgggc ccagtgtcac taggcgggaa cacccagcgc
     2461 gcgtgcgccc tggcaggaag atggctgtga gggacagggg agtggcgccc tgcaatattt
     2521 gcatgtcgct atgtgttctg ggaaatcacc ataaacgtga aatgtctttg gatttgggag
     2581 tcttataagt tctgtatgag accactcttt ccctaggcaa ggtgatgagt cctgttttag
     2641 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
     2701 agtcggtgct tttttaagct tggcgtaact agatcttgag acaaatggca gtattcatcc
     2761 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     2821 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     2881 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag ggggcccggg
     2941 tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg
     3001 tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     3061 cagtccccga gaagttgggg ggaggggtcg gcaattgatc cggtgcctag agaaggtggc
     3121 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     3181 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     3241 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     3301 gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc
     3361 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
     3421 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     3481 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     3541 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     3601 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     3661 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     3721 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     3781 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     3841 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     3901 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     3961 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
     4021 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     4081 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     4141 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgac
     4201 gtacggccac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     4261 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     4321 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     4381 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga
     4441 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     4501 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     4561 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggagt ctcgcccgac caccagggca
     4621 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     4681 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     4741 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     4801 ccggtgccgg atcgggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga
     4861 atcctggccc accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca
     4921 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     4981 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     5041 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     5101 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     5161 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     5221 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     5281 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     5341 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     5401 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     5461 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     5521 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     5581 agctgtacaa gtaaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa
     5641 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5701 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5761 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5821 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     5881 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5941 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     6001 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     6061 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     6121 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     6181 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
     6241 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     6301 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6361 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6421 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6481 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
     6541 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     6601 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6661 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     6721 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6781 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6841 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     6901 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     6961 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     7021 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     7081 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7141 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7201 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7261 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7321 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7381 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7441 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7501 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     7561 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7621 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7681 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7741 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7801 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7861 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7921 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7981 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8041 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8101 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8161 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8221 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8281 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8341 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8401 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8461 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8521 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8581 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8641 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8701 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8761 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8821 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8881 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8941 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9001 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9061 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9121 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9181 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9241 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9301 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9361 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9421 ccgcagccga a
//
