LOCUS       sequence-305795-       10938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding TRV2 with gRNA targeting NbPDS3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10938)
  AUTHORS   Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
            DF
  TITLE     Multiplexed heritable gene editing using RNA viruses and mobile 
            single guide RNAs.
  JOURNAL   Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y.
  PUBMED    32483329
REFERENCE   2  (bases 1 to 10938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y."
COMMENT     Sequence Label: pEE390
FEATURES             Location/Qualifiers
     source          1..10938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      123..317
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(553..575)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    661..801
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     716..735
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(816..833)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(987..1575)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1067..1086)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3000..3794)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    4219..4243
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4772..5117
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5060..5080
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_RNA        6672..6747
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             7213..7230
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      7265..7517
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7539..7563
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8864..9493
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(9922..10938,1..57)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt
       61 acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt
      121 cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac
      181 attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa
      241 gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc
      301 cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct
      361 acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct
      421 ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg
      481 ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga
      541 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
      601 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
      661 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
      721 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
      781 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      841 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      901 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      961 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1021 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1081 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1141 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1201 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1261 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1321 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1381 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     1441 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1501 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1561 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1621 gaaaactcac gttaagggct gatgaatccc ctaatgattt ttatcaaaat cattaagtta
     1681 aggtagatac acatcttgtc atatgatcaa atggtttcgc caaaaatcaa taatcagaca
     1741 acaaaatgtg cgaactcgat attttacacg actctcttta ccaattctgc cccgaattac
     1801 acttaaaacg actcaacagc ttaacgttgg cttgccacgc cttacttgac tgtaaaactc
     1861 tcactcttac cgaacttggc cgtaacctgc caaccaaagc gagaacaaaa cataacatca
     1921 aacgaatcga ccgattgtta ggtaatcgtc acctccacaa agagcgactc gctgtatacc
     1981 gttggcatgc tagctttatc tgttcgggca atacgatgcc cattgtactt gttgactggt
     2041 ctgatatccg tgagcaaaaa cggcttatgg tattgcgagc ttcagtcgca ctacacggtc
     2101 gttctgttac tctttatgag aaagcgttcc cgctttcaga gcaatgttca aagaaagctc
     2161 atgaccaatt tctagccgac cttgcgagca ttctaccgag taacaccaca ccgctcattg
     2221 tcagtgatgc tggctttaaa gtgccatggt ataaatccgt tgagaagctg ggttggtact
     2281 ggttaagtcg agtaagagga aaagtacaat atgcagacct aggagcggaa aactggaaac
     2341 ctatcagcaa cttacatgat atgtcatcta gtcactcaaa gactttaggc tataagaggc
     2401 tgactaaaag caatccaatc tcatgccaaa ttctattgta taaatctcgc tctaaaggcc
     2461 gaaaaaatca gcgctcgaca cggactcatt gtcaccaccc gtcacctaaa atctactcag
     2521 cgtcggcaaa ggagccatgg attctagcaa ctaacttacc tgttgaaatt cgaacaccca
     2581 aacaacttgt taatatctat tcgaagcgaa tgcagattga agaaaccttc cgagacttga
     2641 aaagtcctgc ctacggacta ggcctacgcc atagccgaac gagcagctca gagcgttttg
     2701 atatcatgct gctaatcgcc ctgatgcttc aactaacatg ttggcttgcg ggcgttcatg
     2761 ctcagaaaca aggttgggac aagcacttcc aggctaacac agtcagaaat cgaaacgtac
     2821 tctcaacagt tcgcttaggc atggaagttt tgcggcattc tggctacaca ataacaaggg
     2881 aagactcact cgtggctgca accctgctta ctcaaaatct attcacacat ggttacgttt
     2941 tggggaaatt atgaggggat ctctcagcgt taagggattt tggtcatgca ttctaggtac
     3001 taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc
     3061 agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg
     3121 acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag
     3181 ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag
     3241 acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta
     3301 aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag
     3361 taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg
     3421 atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt
     3481 tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc
     3541 cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat
     3601 agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc
     3661 atttttaaat ataggttttc attttctccc accagcttat ataccttagc aggagacatt
     3721 ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt
     3781 ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag
     3841 aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat
     3901 accagaaaac agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg
     3961 agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac
     4021 atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc
     4081 gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc
     4141 gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
     4201 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca
     4261 acttaataac acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcctag
     4321 gccaccatgt tgggcccggc gcgccaagct tgcatgcctg caggtcaaca tggtggagca
     4381 cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc agagggctat
     4441 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
     4501 ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg
     4561 cgataaagga aaggctatcg ttcaagatgc ctctaccgac agtggtccca aagatggacc
     4621 cccacccacg aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     4681 ggattgatgt gatggtcaac atggtggagc acgacactct cgtctactcc aagaatatca
     4741 aagatacagt ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg
     4801 gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa
     4861 aggaagatgg cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
     4921 cctctaccga cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag
     4981 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     5041 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     5101 ttcatttgga gaggataaaa cattgcacct atggtgttgc cctggctggg gtatgtcagt
     5161 gatcgcagta gaatgtacta attgacaagt tggagaatac ggtagaacgt ccttatccaa
     5221 cacagccttt atccctctcc ctgacgaggt ttttgtcagt gtaatatttc tttttgaact
     5281 atccagctta gtaccgtacg ggaaagtgac tggtgtgctt atctttgaaa tgttactttg
     5341 ggtttcggtt ctttaggtta gtaagaaagc acttgtcttc tcatacaaag gaaaacctga
     5401 gacgtatcgc ttacgaaagt agcaatgaaa gaaaggtggt ggttttaatc gctaccgcaa
     5461 aaacgatggg gtcgttttaa ttaacttctc ctacgcaagc gtctaaacgg acgttggggt
     5521 tttgctagtt tctttagaga aaactagcta agtctttaat gttatcatta gagatggcat
     5581 aaatataata cttgtgtctg ctgataagat cattttaatt tggacgatta gacttgttga
     5641 actacaggtt actgaatcac ttgcgctaat caacatggga gatatgtacg atgaatcatt
     5701 tgacaagtcg ggcggtcctg ctgacttgat ggacgattct tgggtggaat cagtttcgtg
     5761 gaaagatctg ttgaagaagt tacacagcat aaaatttgca ctacagtctg gtagagatga
     5821 gatcactggg ttactagcgg cactgaatag acagtgtcct tattcaccat atgagcagtt
     5881 tccagataag aaggtgtatt tccttttaga ctcacgggct aacagtgctc ttggtgtgat
     5941 tcagaacgct tcagcgttca agagacgagc tgatgagaag aatgcagtgg cgggtgttac
     6001 aaatattcct gcgaatccaa acacaacggt tacgacgaac caagggagta ctactactac
     6061 caaggcgaac actggctcga ctttggaaga agacttgtac acttattaca aattcgatga
     6121 tgcctctaca gctttccaca aatctctaac ttcgttagag aacatggagt tgaagagtta
     6181 ttaccgaagg aactttgaga aagtattcgg gattaagttt ggtggagcag ctgctagttc
     6241 atctgcaccg cctccagcga gtggaggtcc gatacgtcct aatccctagg gatttaagga
     6301 cgtgaactct gttgagatct ctgtgaaatt cagagggtgg gtgataccat attcactgat
     6361 gccattagcg acatctaaat agggctaatt gtgactaatt tgagggaatt tcctttacca
     6421 ttgacgtcag tgtcgttggt agcatttgag tttcggagca tcttgttctg gggtttcaca
     6481 ctatctttag agaaagtgtt aagttaatta agttatctta attaagagca taattatact
     6541 gatttgtctc tcgttgatag agtctatcat tctgttacta aaaatttgac aactcggttt
     6601 gctgacctac tggttactgt atcacttacc cgagttaacg agtctagaaa cttggtagta
     6661 gcgactccat ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     6721 cttgaaaaag tggcaccgag tcggtgcagt gccgggcatg tcccgaagac attaaactac
     6781 ggttctttaa gtagatccgt gtctgaagtt ttaggttcaa tttaaaccta cgagattgac
     6841 attctcgact gatcttgatt gatcggtaag tcttttgtaa tttaattttc tttttgattt
     6901 tattttaaat tgttatctgt ttctgtgtat agactgtttg agatcggcgt ttggccgact
     6961 cattgtctta ccatagggga acggactttg tttgtgttgt tattttattt gtattttatt
     7021 aaaattctca acgatctgaa aaagcctcgc ggctaagaga ttgttggggg gtgagtaagt
     7081 acttttaaag tgatgatggt tacaaaggca aaaggggtaa aacccctcgc ctacgtaagc
     7141 gttattacgc ccgtctgtac ttatatcagt acactgacga gtccctaaag gacgaaacgg
     7201 gagaacgcta gccaccacca ccaccaccac gtgtgaatta caggtgacca gctcgaattt
     7261 ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     7321 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     7381 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     7441 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     7501 atctatgtta ctagatcggg aattaaacta tcagtgtttg acaggatata ttggcgggta
     7561 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
     7621 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
     7681 actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
     7741 tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc
     7801 ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag
     7861 aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc
     7921 gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc
     7981 accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg
     8041 atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc
     8101 cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg
     8161 cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc
     8221 gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg
     8281 cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca
     8341 cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct
     8401 gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg
     8461 acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac
     8521 gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg
     8581 gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt
     8641 acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt
     8701 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
     8761 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     8821 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     8881 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     8941 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     9001 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     9061 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     9121 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     9181 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
     9241 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
     9301 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
     9361 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
     9421 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
     9481 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
     9541 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
     9601 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
     9661 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
     9721 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
     9781 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
     9841 gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg gcactggaac
     9901 ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc
     9961 gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa
    10021 cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc
    10081 aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc
    10141 gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc
    10201 agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg
    10261 atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc
    10321 agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac
    10381 cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac
    10441 gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc
    10501 tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc
    10561 cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc
    10621 gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc
    10681 acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc
    10741 ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga
    10801 tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt
    10861 ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag
    10921 gcggggcagg ctggcccg
//
