LOCUS       sequence-305797-       11479 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding TRV2 with FT augmented gRNA targeting NbAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11479)
  AUTHORS   Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
            DF
  TITLE     Multiplexed heritable gene editing using RNA viruses and mobile 
            single guide RNAs.
  JOURNAL   Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y.
  PUBMED    32483329
REFERENCE   2  (bases 1 to 11479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y."
COMMENT     Sequence Label: pEE387
FEATURES             Location/Qualifiers
     source          1..11479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        427..772
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     715..735
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_RNA        2327..2402
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             3409..3426
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3461..3713
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3735..3759
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5060..5689
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6118..7191
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7257..7451
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7687..7709)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7795..7935
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7850..7869
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7950..7967)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8121..8709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8201..8220)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10134..10928)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11353..11377
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
      781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
      841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
      901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
      961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
     1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
     1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
     1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
     1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
     1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
     1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
     1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
     1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
     1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
     1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
     1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
     1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
     1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
     1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
     1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
     1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
     1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
     2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
     2101 ttgagtttcg gagcatcttg ttctggggtt tcacactatc tttagagaaa gtgttaagtt
     2161 aattaagtta tcttaattaa gagcataatt atactgattt gtctctcgtt gatagagtct
     2221 atcattctgt tactaaaaat ttgacaactc ggtttgctga cctactggtt actgtatcac
     2281 ttacccgagt taacgagtct agaaacgtgt gaaagaaaca attgaggttt tagagctaga
     2341 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     2401 gctcccgcgg ccaccatgtc tataaatata agagaccctc ttatagtaag cagagttgtt
     2461 ggagacgttc ttgatccgtt taatagatca atcactctaa aggttactta tggccaaaga
     2521 gaggtgacta atggcttgga tctaaggcct tctcaggttc aaaacaagcc aagagttgag
     2581 attggtggag aagacctcag gaacttctat actttggtta tggtggatcc agatgttcca
     2641 agtcctagca accctcacct ccgagaatat ctccattggt tggtgactga tatccctgct
     2701 acaactggaa caacctttgg caatgagatt gtgtgttacg aaaatccaag tcccactgca
     2761 ggaattcatc gtgtcgtgtt tatattgttt cgacagcttg gcaggcaaac agtgtatgca
     2821 ccagggtggc gccagaactt caacactcgc gagtttgctg agatctacaa tctcggcctt
     2881 cccgtggccg cagttttcta caattgtcag agggagagtg gctgcggagg aagaagactt
     2941 tagagtgccg ggcatgtccc gaagacatta aactacggtt ctttaagtag atccgtgtct
     3001 gaagttttag gttcaattta aacctacgag attgacattc tcgactgatc ttgattgatc
     3061 ggtaagtctt ttgtaattta attttctttt tgattttatt ttaaattgtt atctgtttct
     3121 gtgtatagac tgtttgagat cggcgtttgg ccgactcatt gtcttaccat aggggaacgg
     3181 actttgtttg tgttgttatt ttatttgtat tttattaaaa ttctcaacga tctgaaaaag
     3241 cctcgcggct aagagattgt tggggggtga gtaagtactt ttaaagtgat gatggttaca
     3301 aaggcaaaag gggtaaaacc cctcgcctac gtaagcgtta ttacgcccgt ctgtacttat
     3361 atcagtacac tgacgagtcc ctaaaggacg aaacgggaga acgctagcca ccaccaccac
     3421 caccacgtgt gaattacagg tgaccagctc gaatttcccc gatcgttcaa acatttggca
     3481 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     3541 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     3601 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     3661 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     3721 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
     3781 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
     3841 gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc
     3901 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac
     3961 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg
     4021 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac
     4081 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg
     4141 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc
     4201 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc
     4261 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac
     4321 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc
     4381 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc
     4441 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc
     4501 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg
     4561 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg
     4621 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc
     4681 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa
     4741 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta
     4801 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg
     4861 tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct
     4921 ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat
     4981 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac
     5041 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg
     5101 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct
     5161 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca
     5221 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg
     5281 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc
     5341 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc
     5401 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca
     5461 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga
     5521 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta
     5581 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc
     5641 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt
     5701 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc
     5761 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt
     5821 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc
     5881 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata
     5941 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg
     6001 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg
     6061 ctgggttgcc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg
     6121 agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
     6181 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
     6241 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
     6301 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
     6361 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
     6421 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
     6481 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
     6541 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
     6601 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
     6661 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
     6721 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
     6781 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
     6841 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
     6901 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
     6961 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
     7021 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
     7081 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
     7141 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
     7201 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg
     7261 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
     7321 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
     7381 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
     7441 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
     7501 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
     7561 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
     7621 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
     7681 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     7741 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     7801 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     7861 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     7921 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7981 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8041 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8101 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8161 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8221 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8281 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8341 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8401 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8461 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8521 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     8581 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8641 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8701 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8761 tcacgttaag ggctgatgaa tcccctaatg atttttatca aaatcattaa gttaaggtag
     8821 atacacatct tgtcatatga tcaaatggtt tcgccaaaaa tcaataatca gacaacaaaa
     8881 tgtgcgaact cgatatttta cacgactctc tttaccaatt ctgccccgaa ttacacttaa
     8941 aacgactcaa cagcttaacg ttggcttgcc acgccttact tgactgtaaa actctcactc
     9001 ttaccgaact tggccgtaac ctgccaacca aagcgagaac aaaacataac atcaaacgaa
     9061 tcgaccgatt gttaggtaat cgtcacctcc acaaagagcg actcgctgta taccgttggc
     9121 atgctagctt tatctgttcg ggcaatacga tgcccattgt acttgttgac tggtctgata
     9181 tccgtgagca aaaacggctt atggtattgc gagcttcagt cgcactacac ggtcgttctg
     9241 ttactcttta tgagaaagcg ttcccgcttt cagagcaatg ttcaaagaaa gctcatgacc
     9301 aatttctagc cgaccttgcg agcattctac cgagtaacac cacaccgctc attgtcagtg
     9361 atgctggctt taaagtgcca tggtataaat ccgttgagaa gctgggttgg tactggttaa
     9421 gtcgagtaag aggaaaagta caatatgcag acctaggagc ggaaaactgg aaacctatca
     9481 gcaacttaca tgatatgtca tctagtcact caaagacttt aggctataag aggctgacta
     9541 aaagcaatcc aatctcatgc caaattctat tgtataaatc tcgctctaaa ggccgaaaaa
     9601 atcagcgctc gacacggact cattgtcacc acccgtcacc taaaatctac tcagcgtcgg
     9661 caaaggagcc atggattcta gcaactaact tacctgttga aattcgaaca cccaaacaac
     9721 ttgttaatat ctattcgaag cgaatgcaga ttgaagaaac cttccgagac ttgaaaagtc
     9781 ctgcctacgg actaggccta cgccatagcc gaacgagcag ctcagagcgt tttgatatca
     9841 tgctgctaat cgccctgatg cttcaactaa catgttggct tgcgggcgtt catgctcaga
     9901 aacaaggttg ggacaagcac ttccaggcta acacagtcag aaatcgaaac gtactctcaa
     9961 cagttcgctt aggcatggaa gttttgcggc attctggcta cacaataaca agggaagact
    10021 cactcgtggc tgcaaccctg cttactcaaa atctattcac acatggttac gttttgggga
    10081 aattatgagg ggatctctca gcgttaaggg attttggtca tgcattctag gtactaaaac
    10141 aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag
    10201 tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag
    10261 aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt
    10321 actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt
    10381 tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga
    10441 gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc
    10501 aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag
    10561 tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct
    10621 tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca
    10681 tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc
    10741 atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt
    10801 aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc
    10861 gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt
    10921 ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
    10981 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
    11041 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
    11101 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
    11161 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
    11221 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
    11281 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
    11341 ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa
    11401 taacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc ctaggccacc
    11461 atgttgggcc cggcgcgcc
//
