LOCUS       sequence-305807-       10848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T-DNA encoding TRV2 with mFT augmented gRNA targeting NbPDS3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10848)
  AUTHORS   Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
            DF
  TITLE     Multiplexed heritable gene editing using RNA viruses and mobile 
            single guide RNAs.
  JOURNAL   Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y.
  PUBMED    32483329
REFERENCE   2  (bases 1 to 10848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y."
COMMENT     Sequence Label: pEE392
FEATURES             Location/Qualifiers
     source          1..10848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        267..342
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             1340..1357
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1392..1644
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1666..1690
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2991..3620
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4049..5122
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5188..5382
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5618..5640)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5726..5866
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5781..5800
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5881..5898)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6052..6640)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6132..6151)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7774..8568)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8993..9017
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        9215..9560
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     9503..9523
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gaatttcctt taccattgac gtcagtgtcg ttggtagcat ttgagtttcg gagcatcttg
       61 ttctggggtt tcacactatc tttagagaaa gtgttaagtt aattaagtta tcttaattaa
      121 gagcataatt atactgattt gtctctcgtt gatagagtct atcattctgt tactaaaaat
      181 ttgacaactc ggtttgctga cctactggtt actgtatcac ttacccgagt taacgagtct
      241 agaaacttgg tagtagcgac tccatggttt tagagctaga aatagcaagt taaaataagg
      301 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctccctagt ctataaatat
      361 aagagaccct cttatagtaa gcagagttgt tggagacgtt cttgatccgt ttaatagatc
      421 aatcactcta aaggttactt atggccaaag agaggtgact aatggcttgg atctaaggcc
      481 ttctcaggtt caaaacaagc caagagttga gattggtgga gaagacctca ggaacttcta
      541 tactttggtt atggtggatc cagatgttcc aagtcctagc aaccctcacc tccgagaata
      601 tctccattgg ttggtgactg atatccctgc tacaactgga acaacctttg gcaatgagat
      661 tgtgtgttac gaaaatccaa gtcccactgc aggaattcat cgtgtcgtgt ttatattgtt
      721 tcgacagctt ggcaggcaaa cagtgtatgc accagggtgg cgccagaact tcaacactcg
      781 cgagtttgct gagatctaca atctcggcct tcccgtggcc gcagttttct acaattgtca
      841 gagggagagt ggctgcggag gaagaagact ttagagtgcc gggcatgtcc cgaagacatt
      901 aaactacggt tctttaagta gatccgtgtc tgaagtttta ggttcaattt aaacctacga
      961 gattgacatt ctcgactgat cttgattgat cggtaagtct tttgtaattt aattttcttt
     1021 ttgattttat tttaaattgt tatctgtttc tgtgtataga ctgtttgaga tcggcgtttg
     1081 gccgactcat tgtcttacca taggggaacg gactttgttt gtgttgttat tttatttgta
     1141 ttttattaaa attctcaacg atctgaaaaa gcctcgcggc taagagattg ttggggggtg
     1201 agtaagtact tttaaagtga tgatggttac aaaggcaaaa ggggtaaaac ccctcgccta
     1261 cgtaagcgtt attacgcccg tctgtactta tatcagtaca ctgacgagtc cctaaaggac
     1321 gaaacgggag aacgctagcc accaccacca ccaccacgtg tgaattacag gtgaccagct
     1381 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     1441 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     1501 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     1561 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     1621 cggtgtcatc tatgttacta gatcgggaat taaactatca gtgtttgaca ggatatattg
     1681 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg
     1741 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga
     1801 tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt
     1861 gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc
     1921 cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg
     1981 cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta
     2041 tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc
     2101 cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct
     2161 ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg
     2221 cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc
     2281 gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt
     2341 gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg
     2401 gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac
     2461 cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga
     2521 ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga
     2581 ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga
     2641 ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac
     2701 caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg
     2761 gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg
     2821 gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc
     2881 gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc
     2941 gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta
     3001 tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc
     3061 gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg
     3121 cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc
     3181 cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag
     3241 cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc
     3301 cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc
     3361 agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca
     3421 aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc
     3481 ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg
     3541 ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa
     3601 ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac
     3661 gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc
     3721 agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct
     3781 gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc
     3841 gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag
     3901 gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg
     3961 gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca
     4021 ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca
     4081 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca
     4141 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
     4201 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc
     4261 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
     4321 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
     4381 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg
     4441 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
     4501 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
     4561 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
     4621 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
     4681 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
     4741 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
     4801 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
     4861 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
     4921 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
     4981 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
     5041 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
     5101 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
     5161 ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc
     5221 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
     5281 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
     5341 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
     5401 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
     5461 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
     5521 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
     5581 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     5641 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     5701 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     5761 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     5821 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     5881 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5941 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6001 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6061 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6121 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6181 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6241 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6301 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6361 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6421 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6481 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6541 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6601 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6661 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggctgatga atcccctaat
     6721 gatttttatc aaaatcatta agttaaggta gatacacatc ttgtcatatg atcaaatggt
     6781 ttcgccaaaa atcaataatc agacaacaaa atgtgcgaac tcgatatttt acacgactct
     6841 ctttaccaat tctgccccga attacactta aaacgactca acagcttaac gttggcttgc
     6901 cacgccttac ttgactgtaa aactctcact cttaccgaac ttggccgtaa cctgccaacc
     6961 aaagcgagaa caaaacataa catcaaacga atcgaccgat tgttaggtaa tcgtcacctc
     7021 cacaaagagc gactcgctgt ataccgttgg catgctagct ttatctgttc gggcaatacg
     7081 atgcccattg tacttgttga ctggtctgat atccgtgagc aaaaacggct tatggtattg
     7141 cgagcttcag tcgcactaca cggtcgttct gttactcttt atgagaaagc gttcccgctt
     7201 tcagagcaat gttcaaagaa agctcatgac caatttctag ccgaccttgc gagcattcta
     7261 ccgagtaaca ccacaccgct cattgtcagt gatgctggct ttaaagtgcc atggattcta
     7321 gcaactaact tacctgttga aattcgaaca cccaaacaac ttgttaatat ctattcgaag
     7381 cgaatgcaga ttgaagaaac cttccgagac ttgaaaagtc ctgcctacgg actaggccta
     7441 cgccatagcc gaacgagcag ctcagagcgt tttgatatca tgctgctaat cgccctgatg
     7501 cttcaactaa catgttggct tgcgggcgtt catgctcaga aacaaggttg ggacaagcac
     7561 ttccaggcta acacagtcag aaatcgaaac gtactctcaa cagttcgctt aggcatggaa
     7621 gttttgcggc attctggcta cacaataaca agggaagact cactcgtggc tgcaaccctg
     7681 cttactcaaa atctattcac acatggttac gttttgggga aattatgagg ggatctctca
     7741 gcgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata
     7801 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
     7861 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
     7921 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
     7981 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
     8041 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
     8101 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
     8161 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
     8221 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
     8281 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
     8341 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
     8401 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
     8461 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
     8521 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
     8581 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
     8641 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
     8701 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca
     8761 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
     8821 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
     8881 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
     8941 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
     9001 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
     9061 taatgtactg aattaacgcc gaattaattc ctaggccacc atgttgggcc cggcgcgcca
     9121 agcttgcatg cctgcaggtc aacatggtgg agcacgacac tctcgtctac tccaagaata
     9181 tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa agggtaatat
     9241 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
     9301 aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct atcgttcaag
     9361 atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac atcgtggaaa
     9421 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
     9481 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
     9541 catttcattt ggagaggata aaacattgca cctatggtgt tgccctggct ggggtatgtc
     9601 agtgatcgca gtagaatgta ctaattgaca agttggagaa tacggtagaa cgtccttatc
     9661 caacacagcc tttatccctc tccctgacga ggtttttgtc agtgtaatat ttctttttga
     9721 actatccagc ttagtaccgt acgggaaagt gactggtgtg cttatctttg aaatgttact
     9781 ttgggtttcg gttctttagg ttagtaagaa agcacttgtc ttctcataca aaggaaaacc
     9841 tgagacgtat cgcttacgaa agtagcaatg aaagaaaggt ggtggtttta atcgctaccg
     9901 caaaaacgat ggggtcgttt taattaactt ctcctacgca agcgtctaaa cggacgttgg
     9961 ggttttgcta gtttctttag agaaaactag ctaagtcttt aatgttatca ttagagatgg
    10021 cataaatata atacttgtgt ctgctgataa gatcatttta atttggacga ttagacttgt
    10081 tgaactacag gttactgaat cacttgcgct aatcaacatg ggagatatgt acgatgaatc
    10141 atttgacaag tcgggcggtc ctgctgactt gatggacgat tcttgggtgg aatcagtttc
    10201 gtggaaagat ctgttgaaga agttacacag cataaaattt gcactacagt ctggtagaga
    10261 tgagatcact gggttactag cggcactgaa tagacagtgt ccttattcac catatgagca
    10321 gtttccagat aagaaggtgt atttcctttt agactcacgg gctaacagtg ctcttggtgt
    10381 gattcagaac gcttcagcgt tcaagagacg agctgatgag aagaatgcag tggcgggtgt
    10441 tacaaatatt cctgcgaatc caaacacaac ggttacgacg aaccaaggga gtactactac
    10501 taccaaggcg aacactggct cgactttgga agaagacttg tacacttatt acaaattcga
    10561 tgatgcctct acagctttcc acaaatctct aacttcgtta gagaacatgg agttgaagag
    10621 ttattaccga aggaactttg agaaagtatt cgggattaag tttggtggag cagctgctag
    10681 ttcatctgca ccgcctccag cgagtggagg tccgatacgt cctaatccct agggatttaa
    10741 ggacgtgaac tctgttgaga tctctgtgaa attcagaggg tgggtgatac catattcact
    10801 gatgccatta gcgacatcta aatagggcta attgtgacta atttgagg
//
