LOCUS       sequence-305810-       13590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, 
            activating DsRed-SKL with promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13590)
  AUTHORS   Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
  TITLE     Synthetic control devices for gene regulation in Penicillium 
            chrysogenum.
  JOURNAL   Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 
            10.1186/s12934-019-1253-3.
  PUBMED    31739777
REFERENCE   2  (bases 1 to 13590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2019-11-18"; volume: "18(1):203. doi"; pages: " 
            10.1186/s12934-019-1253-3"
COMMENT     Sequence Label: pLM2_45_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_0xQUAS
FEATURES             Location/Qualifiers
     source          1..13590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2222..2332
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             join(2345..2347,2348..2350,2351..3061)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2345..2347,2348..2350,2351..3061)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2390..2411)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2651..2670)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2998..3019
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3062..3082
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4254..4369
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             6860..7534
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(6990..7008)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(7040..7060)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     7452..7475
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    9368..9392
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      9480..10190
                     /label=oriV
                     /note="incP origin of replication"
     promoter        10211..10315
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10316..11110
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11281..11869
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11770..11789
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12205..13353
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    13499..13523
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gcctgcagga tgggcctcca caaccctgcc ctaacattgt
       61 tcgtagaaga tctgccagca ggcttatgtc caggcccctc tcaacccgcc ctttcctttc
      121 agctcgggcg ccgtacttgc cccacccagg cgtgttccgc tgaactgccc atacccaggc
      181 cagcggactg ataaccattt ctctttatgc tttgctattg ggcttgtgac tcgttcttgg
      241 aggcaaggtt atcagattga atagaagatt cggtgatgca gcaaatagcg actgttcgtt
      301 gcggggtccg aacccgctcg gcagcaccgg gctctcccta ctatccctcg atagcagtgg
      361 cgtgcgcggg ctgtcgaccc tgtccatcct gaaagagttg atattgaagg tagtaggtac
      421 ggtgcagggt aacgcgtgat atcctgtcct cagtcttaag acttctcacc tatatcaccc
      481 gcacttaccc tagagtgccg cttaggtgct aagggcacat tgagtattgg ccgtgtagaa
      541 tatatagctt aagtacggcc aagcagacgg gaagccctgt tctccacacc ctatggtcgt
      601 atatatcagg cttctaccgg gaaacgatta agagtgtata atggactgaa aatcaatatg
      661 aacgggacaa tgctcaagtt aaattagtta ggcatcctaa tctctactaa atgttctatc
      721 tagagatcgg ggtactatag gcccgtacgt taatcactct acgcttctct cccttaggta
      781 tagtgtaggt aggggctaga catttatatg agtcagatgg tacaaacggt agcgctgcaa
      841 gaattcaagc ttggagtctt gcgttacggg cgtattttgc tgcggccggt ggtgcccctc
      901 catgccccgc catctttcaa agctcctggc gacgccgtca tctccgaaca ttctcccccc
      961 aaaggaatca attggcaatt ggagtctagt aaagtggtgt ttgtcatcag taaggagttg
     1021 gtgaaactac aatcttccat catgaagaga agggatattt ttggggttgt attttacgat
     1081 gaaggtactg gaaatggtgg gggtttttat agcagtagac agtcagtcag taagtagtat
     1141 gcttgttgta ttacccaaac cagatcaatc caaagaaagc ctgacagaca gccatcaata
     1201 gatactactt cgtactatag ttacccacct aaccatatta ctcaaaaagc atctatctat
     1261 ccgcgggctt ccatgcatgt cccggtagca aactcctccc accggtgtag tactctttgg
     1321 ttagtagtct tgttcaccgg aggactctgc tcctctcctg ctcaggtgct gccccgccct
     1381 ccgtcccacc atgacggaag agatgctccg taagccgtcc agttgcaacg aatcctgctc
     1441 tgacatcttc gaacgccttc tccctttcgc tcgcttctct gcctctttcc tctcttccct
     1501 ttccttcccc tccaaactaa accttcctcc ttttctccat catcctctag gcagttggtt
     1561 cttcctgact gtacatatat ccaccacctc ccccctctat tcttccacct cttccatatc
     1621 tccttctcca gagttcatac cccccacaat gccacccaag cgcaaaacgc ttaacgctgc
     1681 ggctgaggct aacgctcatg ccgacggaca cgccgacgga aacgccgacg gacacgtggc
     1741 caatacggcc gcgtcctcga ataatgcgag gttcgctgat ctcactaaca tcgatactcc
     1801 gggtctggga cccacaacta cgaccctgct cgtggaacca gcacgctcaa agcgtcaacg
     1861 agtgtcccgc gcatgcgacc agtgccgtgc agcccgagag aaatgcgacg gaatacagcc
     1921 tgcgtgtttc ccgtgcgttt cccagggaag gtcctgcact tatcaggctt cgccgaaaaa
     1981 gaggggagtt caaaccggtt atattcgtac gctggagctc gccctcgcct ggatgtttga
     2041 aaatgtcgcg cgttccgaag atgccttgca taacctcctc gtccgtgacg ccggacaagg
     2101 atcagctctg ctcgttggta aagattcgcc ggctgccgag cgactccatg cccgttgggc
     2161 tactagccgt gtcaataaga gcattacccg cctcctcagg tttgaaagcg gcgggccggc
     2221 cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga
     2281 ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggtt
     2341 cgttatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2401 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2461 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     2521 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     2581 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2641 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2701 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2761 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     2821 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     2881 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     2941 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3001 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3061 gccaaagaag aagcggaagg tctaagctta cttctttatc ggttctctct tacgactttt
     3121 tgaatggaac gtttccttct tctcaggcgg gcctatcttt gggccgaagc tcttttcctt
     3181 gtactgtagg acctggttga taatgattcc caaaaagaca tccagcatgt cagttacttg
     3241 cattcgtcag tctatacaaa agcaatggtt tagagaaatt ttgaacttta tacatggttt
     3301 tatttgttgc ttcacggccg taccttctgg aaatccacgg taggagtgtc aatttgcgtt
     3361 tttgataatc cttccaaggt tcttctcgaa gtagttgttc tataattgct tcacagctac
     3421 catggaacat cccaagcacc gctactagaa ttcgagctcg gagtggatcc cccgggctgc
     3481 aggaattcga gctctgtaca gtgaccggtg actctttctg gcatgcggag agacggacgg
     3541 acgcagagag aagggctgag taataagcgc cactgcgcca gacagctctg gcggctctga
     3601 ggtgcagtgg atgattatta atccgggacc ggccgcccct ccgccccgaa gtggaaaggc
     3661 tggtgtgccc ctcgttgacc aagaatctat tgcatcatcg gagaatatgg agcttcatcg
     3721 aatcaccggc agtaagcgaa ggagaatgtg aagccagggg tgtatagccg tcggcgaaat
     3781 agcatgccat taacctaggt acagaagtcc aattgcttcc gatctggtaa aagattcacg
     3841 agatagtacc ttctccgaag taggtagagc gagtacccgg cgcgtaagct ccctaattgg
     3901 cccatccggc atctgtaggg cgtccaaata tcgtgcctct cctgctttgc ccggtgtatg
     3961 aaaccggaaa ggccgctcag gagctggcca gcggcgcaga ccgggaacac aagctggcag
     4021 tcgacccatc cggtgctctg cactcgacct gctgaggtcc ctcagtccct ggtaggcagc
     4081 tttgccccgt ctgtccgccc ggtgtgtcgg cggggttgac aaggtcgttg cgtcagtcca
     4141 acatttgttg ccatattttc ctgctctccc caccagctgc tcttttcttt tctctttctt
     4201 ttcccatctt cagtatattc atcttcccat ccaagaacct ttatttcccc taagtaagta
     4261 ctttgctaca tccatactcc atccttccca tcccttattc ctttgaacct ttcagttcga
     4321 gctttcccac ttcatcgcag cttgactaac agctaccccg cttgagcaga catcaccatg
     4381 cctcaatcct gggaagaact ggccgctgat aagcgcgccc gcctcgcaaa aaccatccct
     4441 gatgaatgga aagtccagac gctgcctgcg gatgacagcg ttattgattt cccaaagaaa
     4501 tcggggatcc tttcagaggc cgaactgaag atcacagagg cctccgctgc agatcttgtg
     4561 tccaagctgg cggccggaga gttgacctcg gtggaagtta cgctagcatt ctgtaaacgg
     4621 gcagcaatcg cccagcagtt agtagggtcc cctctacctc tcagggagat gtaacaacgc
     4681 caccttatgg gactatcaag ctgacgctgg cttctgtgca gacaaactgc gcccacgagt
     4741 tcttccctga cgccgctctc gcgcaggcaa gggaactcga tgaatactac gcaaagcaca
     4801 agacacccgt tggtccactc catggcctcc ccatctctct caaagaccag cttcgagtca
     4861 aggtacaccg ttgcccctaa gtcgttagat gtcccttttt gtcagctaac atatgccacc
     4921 agggctacga aacatcaatg ggctacatct catggctaaa caagtacgac gaaggggact
     4981 cggttctgac aaccatgctc cgcaaagccg gtgccgtttt ctacgtcaag acctctgtcc
     5041 cgcagaccct gatggtctgc gagacagtca acaacatcat cgggcgcacc gtcaacccac
     5101 gcaacaagaa ctggtcgtgc ggcggcagtt ctggtggtga gggtgcgatc gttgggattc
     5161 gtggtggcgt catcggtgta ggaacggata tcggtggctc gattcgagtg ccggccgcgt
     5221 tcaacttcct gtacggtcta aggccgagtc atgggcggct gccgtatgca aagatggcga
     5281 acagcatgga gggtcaggag acggtgcaca gcgttgtcgg gccgattacg cactctgttg
     5341 agggtgagtc cttcgcctct tccttctttt cctgctctat accaggcctc cactgtcctc
     5401 ctttcttgct ttttatacta tatacgagac gggcagtcac tgatgaagta atgttagacc
     5461 tccgcctctt caccaaatcc gtcctcggtc aggagccatg gaaatacgac tccaaggtca
     5521 tccccatgcc ctggcgccag tccgagtcgg acattattgc ctccaagatc aagaacggcg
     5581 ggctcaatat cggctactac aacttcgacg gcaatgtcct tccacaccct cctatcctgc
     5641 gcggcgtgga aaccaccgtc gccgcactcg ccaaagccgg tcacaccgtg accccgtgga
     5701 cgccatacaa gcacgatttc ggccacgatc tcatctccca tatctacgcg gctgacggca
     5761 gcgccgacgt aatgcgcgat atcagtgcat ccggcgagcc ggcgattcca aatatcaaag
     5821 acctactgaa cccgaacatc aaagctgtta acatgaacga gctctgggac acgcatctcc
     5881 agaagtggaa ttaccagatg gagtaccttg agaaatggcg ggaggctgaa gaaaaggccg
     5941 ggaaggaact ggacgccatc atcgcgccga ttacgcctac cgctgcggta cggcatgacc
     6001 agttccggta ctatgggtat gcctctgtga tcaacctgct ggatttcacg agcgtggttg
     6061 ttccggttac ctttgcggat aagaacatcg ataagaagaa tgagagtttc aaggcggtta
     6121 gtgagcttga tgccctcgtg caggaagagt atgatccgga ggcgtaccat ggggcaccgg
     6181 ttgcagtgca ggttatcgga cggagactca gtgaagagag gacgttggcg attgcagagg
     6241 aagtggggaa gttgctggga aatgtggtga ctccataggc ttctaataag tgtcagatag
     6301 caatttgcac aagaaatcaa taccagcaac tgtaaataag cgctgaagtg accatgccat
     6361 gctacgaaag agcagaaaaa aacctgccgt agaaccgaag agatatgaca cgcttccatc
     6421 tctcaaagga agaatccctt cagggttgcg tttccagtct agacacgtat aacggcacaa
     6481 gtgtctctca ccaaatgggt tatatctcaa atgtgatcta aggatggaaa gcccagaata
     6541 ttggctgggt tgatggctgc ttcgagtgca gtctcatgct gccacaggtg actctggatg
     6601 gccccatacc actcaaccca tggtacgagc ggtacgcttt acgaattccc atggggagcg
     6661 tataatgtct ccaggttgtc tcagcataaa caccccgccc ccgctcaggc acacaggaag
     6721 agagctcagg tcgtttccat tgcgtccata ctcttcactc attgtcatct gcaggagaac
     6781 ttcccctgtc cctttgccaa gccctctctt cgtcgttgtc cacgccttca agttttcacc
     6841 attatttttc tagacaccaa tggcctcctc cgaggacgtc atcaaggagt tcatgcgctt
     6901 caaggtgcgc atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     6961 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct
     7021 gcccttcgcc tgggacatcc tgtcccccca gttccagtac ggctccaagg tgtacgtgaa
     7081 gcaccccgcc gacatccccg actacaagaa gctgtccttc cccgagggct tcaagtggga
     7141 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     7201 ggacggctcc ttcatctaca aggtgaagtt catcggcgtg aacttcccct ccgacggccc
     7261 cgtaatgcag aagaaaacta tgggctggga ggcctccacc gagcgcctgt acccccgcga
     7321 cggcgtgctg aagggcgaga tccacaaggc cctgaagctg aaggacggcg gccactacct
     7381 ggtggagttc aagtccatct acatggccaa gaagcccgtg cagctgcccg gctactacta
     7441 cgtggactcc aagctggaca tcacctccca caacgaggac tacaccatcg tggagcagta
     7501 cgagcgcgcc gagggccgcc accacctgtt cctgactcac ggtatggacg aattgtacaa
     7561 gtcgaagctg taagcttgtg cttctaaggt atgagtcgca aaattgtttt ttatttttgg
     7621 tcttgagtct aatatgctcg cagctcttgc gttgtatatg gtcgttgtcg cgtattttct
     7681 gttgtattaa aagatcaaac gagatcaagg gatggctcgc gggctgtctc tcgcactagg
     7741 aggaagaatg cctgaaaaag gaactttgat tttagctgtg gaatagagat ggcttgtttg
     7801 aggacgcttg tcgcttggcg cagggacttg aatggcagct tgtggaaacc gaaggcgaga
     7861 aaagtcgacg gatactgtac gtggttctat tgccagtgcg gtggaagctt ccttctcata
     7921 tagttcaatc cttctttgaa tctgtttgtt tcatatttgg actgtttcat tctctgcttg
     7981 cgcattctca tcttcgagaa cgactgcagg gattgttggt tctgtggagc tgatgagcgc
     8041 gccttgacca cccttgttct tgttttgctc ttttgttctc atttaacccg tttctccctt
     8101 ccaacccttt gaccttgcaa cattgtctcc cagcgcgttg ccaaagcgaa cttgatatca
     8161 gtatagtatg accaagtagt ctaccaaaat aaattttagt acagtattgc tagttataca
     8221 agataaattt tgtacaacaa tgattatgca gagctggtat tctgtaagga gtaaattcat
     8281 gcaggtacag agcgtccacg tcaggcacac gcgctttagt gctgggtaca cattatgcta
     8341 cctttggggc aaggtgctat tctaggtagg gtatgcctag caatgccatg atcttatgac
     8401 ctcagtaatc tgaccttgga tttaagatcg gagttctcaa accctgcgcg ctcagagaat
     8461 tcgcatgcgg agactttaga catacctctc gttgccgaca aatcggcatt ccgtttcggt
     8521 cacggacgta agagttcaac aaagccgctc acctgtcccg ttcggcgctt gagtttccac
     8581 ttcaaaagca caaccgctcc gatatttcca ttgccaccga ccaacaaaag gttcatatcg
     8641 cgcaatagtc gtccaactct cactccgccc gcactcgatg gcgaaggatg gaagaatatc
     8701 cgagcggcat cggataaaga gatctggttc ttttcgtgaa gtaacgtagg gaaccgacgg
     8761 aaactgtaga gctataggaa tttagtatat cagaagtcaa ttgagagaag tgtatagtta
     8821 gtaactcact tgtgccaaca ggtatttcca accgttcttg ttcaaatttc gtcaagtcac
     8881 catctctatg tgcaaaactg gatcatacaa cagctcttat tgtagctgca tctcgctctc
     8941 agagatcatc ctatcttcgt cgtcatccac caacacaggt gggagtgcta gttgatccat
     9001 gctgaggtca agggacacag acggaatggg tgtagaacgc ctcaaaacag gtaaggtatc
     9061 aggatcggtt gtccaggggg aatccaaacc aggttccacc ttgattcaga gaataagtat
     9121 gacgtattct tggcaatgtt taagcttgag atgagccgat gtcaaactgg gagacatgat
     9181 gaaggaggaa atgttgcttt ggtgggggag acactataaa taccaaagag tgaatggttg
     9241 ataccatttg gggaaggagt gtctatcgca gtctctgcag acggcatatc ggatagcctg
     9301 caggcgcttg tggaggatgc acatgtgacc gagggacacg aagtgatccg tttaaactat
     9361 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata
     9421 atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgccagcc
     9481 gcctttgcga cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc
     9541 cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt
     9601 tgtggatacc tcgcggaaaa cttggccctc actgacagat gaggggcgga cgttgacact
     9661 tgaggggccg actcacccgg cgcggcgttg acagatgagg ggcaggctcg atttcggccg
     9721 gcgacgtgga gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc
     9781 ccacagatga tgtggacaag cctggggata agtgccctgc ggtattgaca cttgaggggc
     9841 gcgactactg acagatgagg ggcgcgatcc ttgacacttg aggggcagag tgctgacaga
     9901 tgaggggcgc acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc
     9961 aagggtttcc gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca
    10021 atatttataa accttgtttt taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg
    10081 aaggggggtg cccccccttc tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc
    10141 ccaggggctg cgcccctcgg ccgcgaacgg cctcacccca aaaatggcag cgctggccaa
    10201 ttcccgagtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    10261 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatggc
    10321 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga
    10381 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata
    10441 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga
    10501 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca
    10561 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta
    10621 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt
    10681 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga
    10741 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagagga
    10801 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga
    10861 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa
    10921 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc
    10981 cttctgcgtc cggtcgctca gggaggatat cggggaagaa cagtatgtcg agctattttt
    11041 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
    11101 attgttttag ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
    11161 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    11221 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    11281 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    11341 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    11401 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
    11461 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    11521 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    11581 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    11641 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    11701 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    11761 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    11821 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    11881 cgcggccttt ttacggttcc tggcagatcc tagatgtggc gcaacgatgc cggcgacaag
    11941 caggagcgca ccgacttctt ccgcatcaag tgttttggct ctcaggccga ggcccacggc
    12001 aagtatttgg gcaaggggtc gctggtattc gtgcagggca agattcggaa taccaagtac
    12061 gagaaggacg gccagacggt ctacgggacc gacttcattg ccgataaggt ggattatctg
    12121 gacaccaagg caccaggcgg gtcaaatcag gaataagggc acattgcccc ggcgtgagtc
    12181 ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg accggaaggc atacaggcaa
    12241 gaactgatcg acgcggggtt ttccgccgag gatgccgaaa ccatcgcaag ccgcaccgtc
    12301 atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga tggtccagca agctacggcc
    12361 aagatcgagc gcgacagcgt gcaactggct ccccctgccc tgcccgcgcc atcggccgcc
    12421 gtggagcgtt cgcgtcgtct tgaacaggag gcggcaggtt tggcgaagtc gatgaccatc
    12481 gacacgcgag gaactatgac gaccaagaag cgaaaaaccg ccggcgagga cctggcaaaa
    12541 caggtcagcg aggccaagca ggccgcgttg ctgaaacaca cgaagcagca gatcaaggaa
    12601 atgcagcttt ccttgttcga tattgcgccg tggccggaca cgatgcgagc gatgccaaac
    12661 gacacggccc gctctgccct gttcaccacg cgcaacaaga aaatcccgcg cgaggcgctg
    12721 caaaacaagg tcattttcca cgtcaacaag gacgtgaaga tcacctacac cggcgtcgag
    12781 ctgcgggccg acgatgacga actggtgtgg cagcaggtgt tggagtacgc gaagcgcacc
    12841 cctatcggcg agccgatcac cttcacgttc tacgagcttt gccaggacct gggctggtcg
    12901 atcaatggcc ggtattacac gaaggccgag gaatgcctgt cgcgcctaca ggcgacggcg
    12961 atgggcttca cgtccgaccg cgttgggcac ctggaatcgg tgtcgctgct gcaccgcttc
    13021 cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg tcctgatcga cgaggaaatc
    13081 gtcgtgctgt ttgctggcga ccactacacg aaattcatat gggagaagta ccgcaagctg
    13141 tcgccgacgg cccgacggat gttcgactat ttcagctcgc accgggagcc gtacccgctc
    13201 aagctggaaa ccttccgcct catgtgcgga tcggattcca cccgcgtgaa gaagtggcgc
    13261 gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg gcctggtgga acacgcctgg
    13321 gtcaatgatg acctggtgca ttgcaaacgc tagggccttg tggggtcagt tccggctggg
    13381 ggttcagcag cccctgctcg gatctgttgg accggacagt agtcatggtt gatgggctgc
    13441 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
    13501 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
    13561 cgtttttaat gtactggggt tgaacactct
//
