LOCUS       sequence-305812-       11479 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding TRV2 with FT augmented gRNA targeting NbPDS3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11479)
  AUTHORS   Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
            DF
  TITLE     Multiplexed heritable gene editing using RNA viruses and mobile 
            single guide RNAs.
  JOURNAL   Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y.
  PUBMED    32483329
REFERENCE   2  (bases 1 to 11479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y."
COMMENT     Sequence Label: pEE391
FEATURES             Location/Qualifiers
     source          1..11479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_RNA        1901..1976
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             2983..3000
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3035..3287
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3309..3333
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4634..5263
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5692..6765
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6831..7025
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7261..7283)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7369..7509
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7424..7443
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7524..7541)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7695..8283)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7775..7794)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9708..10502)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10927..10951
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ctctaccgac agtggtccca aagatggacc
      181 cccacccacg aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggataaaac attgcaccta
      361 tggtgttgcc ctggctgggg tatgtcagtg atcgcagtag aatgtactaa ttgacaagtt
      421 ggagaatacg gtagaacgtc cttatccaac acagccttta tccctctccc tgacgaggtt
      481 tttgtcagtg taatatttct ttttgaacta tccagcttag taccgtacgg gaaagtgact
      541 ggtgtgctta tctttgaaat gttactttgg gtttcggttc tttaggttag taagaaagca
      601 cttgtcttct catacaaagg aaaacctgag acgtatcgct tacgaaagta gcaatgaaag
      661 aaaggtggtg gttttaatcg ctaccgcaaa aacgatgggg tcgttttaat taacttctcc
      721 tacgcaagcg tctaaacgga cgttggggtt ttgctagttt ctttagagaa aactagctaa
      781 gtctttaatg ttatcattag agatggcata aatataatac ttgtgtctgc tgataagatc
      841 attttaattt ggacgattag acttgttgaa ctacaggtta ctgaatcact tgcgctaatc
      901 aacatgggag atatgtacga tgaatcattt gacaagtcgg gcggtcctgc tgacttgatg
      961 gacgattctt gggtggaatc agtttcgtgg aaagatctgt tgaagaagtt acacagcata
     1021 aaatttgcac tacagtctgg tagagatgag atcactgggt tactagcggc actgaataga
     1081 cagtgtcctt attcaccata tgagcagttt ccagataaga aggtgtattt ccttttagac
     1141 tcacgggcta acagtgctct tggtgtgatt cagaacgctt cagcgttcaa gagacgagct
     1201 gatgagaaga atgcagtggc gggtgttaca aatattcctg cgaatccaaa cacaacggtt
     1261 acgacgaacc aagggagtac tactactacc aaggcgaaca ctggctcgac tttggaagaa
     1321 gacttgtaca cttattacaa attcgatgat gcctctacag ctttccacaa atctctaact
     1381 tcgttagaga acatggagtt gaagagttat taccgaagga actttgagaa agtattcggg
     1441 attaagtttg gtggagcagc tgctagttca tctgcaccgc ctccagcgag tggaggtccg
     1501 atacgtccta atccctaggg atttaaggac gtgaactctg ttgagatctc tgtgaaattc
     1561 agagggtggg tgataccata ttcactgatg ccattagcga catctaaata gggctaattg
     1621 tgactaattt gagggaattt cctttaccat tgacgtcagt gtcgttggta gcatttgagt
     1681 ttcggagcat cttgttctgg ggtttcacac tatctttaga gaaagtgtta agttaattaa
     1741 gttatcttaa ttaagagcat aattatactg atttgtctct cgttgataga gtctatcatt
     1801 ctgttactaa aaatttgaca actcggtttg ctgacctact ggttactgta tcacttaccc
     1861 gagttaacga gtctagaaac ttggtagtag cgactccatg gttttagagc tagaaatagc
     1921 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctccc
     1981 gcggccacca tgtctataaa tataagagac cctcttatag taagcagagt tgttggagac
     2041 gttcttgatc cgtttaatag atcaatcact ctaaaggtta cttatggcca aagagaggtg
     2101 actaatggct tggatctaag gccttctcag gttcaaaaca agccaagagt tgagattggt
     2161 ggagaagacc tcaggaactt ctatactttg gttatggtgg atccagatgt tccaagtcct
     2221 agcaaccctc acctccgaga atatctccat tggttggtga ctgatatccc tgctacaact
     2281 ggaacaacct ttggcaatga gattgtgtgt tacgaaaatc caagtcccac tgcaggaatt
     2341 catcgtgtcg tgtttatatt gtttcgacag cttggcaggc aaacagtgta tgcaccaggg
     2401 tggcgccaga acttcaacac tcgcgagttt gctgagatct acaatctcgg ccttcccgtg
     2461 gccgcagttt tctacaattg tcagagggag agtggctgcg gaggaagaag actttagagt
     2521 gccgggcatg tcccgaagac attaaactac ggttctttaa gtagatccgt gtctgaagtt
     2581 ttaggttcaa tttaaaccta cgagattgac attctcgact gatcttgatt gatcggtaag
     2641 tcttttgtaa tttaattttc tttttgattt tattttaaat tgttatctgt ttctgtgtat
     2701 agactgtttg agatcggcgt ttggccgact cattgtctta ccatagggga acggactttg
     2761 tttgtgttgt tattttattt gtattttatt aaaattctca acgatctgaa aaagcctcgc
     2821 ggctaagaga ttgttggggg gtgagtaagt acttttaaag tgatgatggt tacaaaggca
     2881 aaaggggtaa aacccctcgc ctacgtaagc gttattacgc ccgtctgtac ttatatcagt
     2941 acactgacga gtccctaaag gacgaaacgg gagaacgcta gccaccacca ccaccaccac
     3001 gtgtgaatta caggtgacca gctcgaattt ccccgatcgt tcaaacattt ggcaataaag
     3061 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     3121 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     3181 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     3241 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattaaacta
     3301 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     3361 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
     3421 caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt
     3481 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc
     3541 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt
     3601 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc
     3661 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac
     3721 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc
     3781 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt
     3841 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc
     3901 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc
     3961 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt
     4021 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag
     4081 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac
     4141 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg
     4201 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc
     4261 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa
     4321 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag
     4381 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa
     4441 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg
     4501 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt
     4561 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac
     4621 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac
     4681 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt
     4741 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct
     4801 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg
     4861 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa
     4921 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc
     4981 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc
     5041 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct
     5101 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg
     5161 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga
     5221 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag
     5281 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag
     5341 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg
     5401 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc
     5461 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag
     5521 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga
     5581 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt
     5641 tgcctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt
     5701 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag
     5761 ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa
     5821 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt
     5881 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg
     5941 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg
     6001 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta
     6061 gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg
     6121 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc
     6181 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc
     6241 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc
     6301 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc
     6361 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc
     6421 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg
     6481 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca
     6541 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt
     6601 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat
     6661 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg
     6721 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg
     6781 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc
     6841 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca
     6901 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc
     6961 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt
     7021 gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg
     7081 ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa
     7141 atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc
     7201 gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa
     7261 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     7321 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     7381 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
     7441 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     7501 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7561 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7621 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7681 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7741 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7801 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7861 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7921 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7981 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8041 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8101 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     8161 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8221 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8281 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8341 taagggctga tgaatcccct aatgattttt atcaaaatca ttaagttaag gtagatacac
     8401 atcttgtcat atgatcaaat ggtttcgcca aaaatcaata atcagacaac aaaatgtgcg
     8461 aactcgatat tttacacgac tctctttacc aattctgccc cgaattacac ttaaaacgac
     8521 tcaacagctt aacgttggct tgccacgcct tacttgactg taaaactctc actcttaccg
     8581 aacttggccg taacctgcca accaaagcga gaacaaaaca taacatcaaa cgaatcgacc
     8641 gattgttagg taatcgtcac ctccacaaag agcgactcgc tgtataccgt tggcatgcta
     8701 gctttatctg ttcgggcaat acgatgccca ttgtacttgt tgactggtct gatatccgtg
     8761 agcaaaaacg gcttatggta ttgcgagctt cagtcgcact acacggtcgt tctgttactc
     8821 tttatgagaa agcgttcccg ctttcagagc aatgttcaaa gaaagctcat gaccaatttc
     8881 tagccgacct tgcgagcatt ctaccgagta acaccacacc gctcattgtc agtgatgctg
     8941 gctttaaagt gccatggtat aaatccgttg agaagctggg ttggtactgg ttaagtcgag
     9001 taagaggaaa agtacaatat gcagacctag gagcggaaaa ctggaaacct atcagcaact
     9061 tacatgatat gtcatctagt cactcaaaga ctttaggcta taagaggctg actaaaagca
     9121 atccaatctc atgccaaatt ctattgtata aatctcgctc taaaggccga aaaaatcagc
     9181 gctcgacacg gactcattgt caccacccgt cacctaaaat ctactcagcg tcggcaaagg
     9241 agccatggat tctagcaact aacttacctg ttgaaattcg aacacccaaa caacttgtta
     9301 atatctattc gaagcgaatg cagattgaag aaaccttccg agacttgaaa agtcctgcct
     9361 acggactagg cctacgccat agccgaacga gcagctcaga gcgttttgat atcatgctgc
     9421 taatcgccct gatgcttcaa ctaacatgtt ggcttgcggg cgttcatgct cagaaacaag
     9481 gttgggacaa gcacttccag gctaacacag tcagaaatcg aaacgtactc tcaacagttc
     9541 gcttaggcat ggaagttttg cggcattctg gctacacaat aacaagggaa gactcactcg
     9601 tggctgcaac cctgcttact caaaatctat tcacacatgg ttacgttttg gggaaattat
     9661 gaggggatct ctcagcgtta agggattttg gtcatgcatt ctaggtacta aaacaattca
     9721 tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa
     9781 aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca
     9841 atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg
     9901 ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct
     9961 tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct
    10021 tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg
    10081 gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag
    10141 agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac
    10201 tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc
    10261 cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc
    10321 ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat
    10381 aggttttcat tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct
    10441 tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc
    10501 atttattatt tccttcctct tttctacagt atttaaagat accccaagaa gctaattata
    10561 acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag
    10621 ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga
    10681 aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc
    10741 gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg
    10801 taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga
    10861 tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct
    10921 ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac
    10981 attgcggacg tttttaatgt actgaattaa cgccgaatta attcctaggc caccatgttg
    11041 ggcccggcgc gccaagcttg catgcctgca ggtcaacatg gtggagcacg acactctcgt
    11101 ctactccaag aatatcaaag atacagtctc agaagaccag agggctattg agacttttca
    11161 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
    11221 cgaaaggaca gtagaaaagg aagatggctt ctacaaatgc catcattgcg ataaaggaaa
    11281 ggctatcgtt caagatgcct ctaccgacag tggtcccaaa gatggacccc cacccacgag
    11341 gaacatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
    11401 tggtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
    11461 cagaagacca gagggctat
//
