LOCUS       sequence-305817-       10938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding TRV2 with gRNA targeting NbAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10938)
  AUTHORS   Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
            DF
  TITLE     Multiplexed heritable gene editing using RNA viruses and mobile 
            single guide RNAs.
  JOURNAL   Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y.
  PUBMED    32483329
REFERENCE   2  (bases 1 to 10938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y."
COMMENT     Sequence Label: pEE386
FEATURES             Location/Qualifiers
     source          1..10938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_RNA        1901..1976
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             2442..2459
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2494..2746
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2768..2792
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4093..4722
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5151..6224
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6290..6484
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6720..6742)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6828..6968
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6883..6902
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6983..7000)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7154..7742)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7234..7253)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9167..9961)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10386..10410
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ctctaccgac agtggtccca aagatggacc
      181 cccacccacg aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggataaaac attgcaccta
      361 tggtgttgcc ctggctgggg tatgtcagtg atcgcagtag aatgtactaa ttgacaagtt
      421 ggagaatacg gtagaacgtc cttatccaac acagccttta tccctctccc tgacgaggtt
      481 tttgtcagtg taatatttct ttttgaacta tccagcttag taccgtacgg gaaagtgact
      541 ggtgtgctta tctttgaaat gttactttgg gtttcggttc tttaggttag taagaaagca
      601 cttgtcttct catacaaagg aaaacctgag acgtatcgct tacgaaagta gcaatgaaag
      661 aaaggtggtg gttttaatcg ctaccgcaaa aacgatgggg tcgttttaat taacttctcc
      721 tacgcaagcg tctaaacgga cgttggggtt ttgctagttt ctttagagaa aactagctaa
      781 gtctttaatg ttatcattag agatggcata aatataatac ttgtgtctgc tgataagatc
      841 attttaattt ggacgattag acttgttgaa ctacaggtta ctgaatcact tgcgctaatc
      901 aacatgggag atatgtacga tgaatcattt gacaagtcgg gcggtcctgc tgacttgatg
      961 gacgattctt gggtggaatc agtttcgtgg aaagatctgt tgaagaagtt acacagcata
     1021 aaatttgcac tacagtctgg tagagatgag atcactgggt tactagcggc actgaataga
     1081 cagtgtcctt attcaccata tgagcagttt ccagataaga aggtgtattt ccttttagac
     1141 tcacgggcta acagtgctct tggtgtgatt cagaacgctt cagcgttcaa gagacgagct
     1201 gatgagaaga atgcagtggc gggtgttaca aatattcctg cgaatccaaa cacaacggtt
     1261 acgacgaacc aagggagtac tactactacc aaggcgaaca ctggctcgac tttggaagaa
     1321 gacttgtaca cttattacaa attcgatgat gcctctacag ctttccacaa atctctaact
     1381 tcgttagaga acatggagtt gaagagttat taccgaagga actttgagaa agtattcggg
     1441 attaagtttg gtggagcagc tgctagttca tctgcaccgc ctccagcgag tggaggtccg
     1501 atacgtccta atccctaggg atttaaggac gtgaactctg ttgagatctc tgtgaaattc
     1561 agagggtggg tgataccata ttcactgatg ccattagcga catctaaata gggctaattg
     1621 tgactaattt gagggaattt cctttaccat tgacgtcagt gtcgttggta gcatttgagt
     1681 ttcggagcat cttgttctgg ggtttcacac tatctttaga gaaagtgtta agttaattaa
     1741 gttatcttaa ttaagagcat aattatactg atttgtctct cgttgataga gtctatcatt
     1801 ctgttactaa aaatttgaca actcggtttg ctgacctact ggttactgta tcacttaccc
     1861 gagttaacga gtctagaaac gtgtgaaaga aacaattgag gttttagagc tagaaatagc
     1921 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgcagtg
     1981 ccgggcatgt cccgaagaca ttaaactacg gttctttaag tagatccgtg tctgaagttt
     2041 taggttcaat ttaaacctac gagattgaca ttctcgactg atcttgattg atcggtaagt
     2101 cttttgtaat ttaattttct ttttgatttt attttaaatt gttatctgtt tctgtgtata
     2161 gactgtttga gatcggcgtt tggccgactc attgtcttac cataggggaa cggactttgt
     2221 ttgtgttgtt attttatttg tattttatta aaattctcaa cgatctgaaa aagcctcgcg
     2281 gctaagagat tgttgggggg tgagtaagta cttttaaagt gatgatggtt acaaaggcaa
     2341 aaggggtaaa acccctcgcc tacgtaagcg ttattacgcc cgtctgtact tatatcagta
     2401 cactgacgag tccctaaagg acgaaacggg agaacgctag ccaccaccac caccaccacg
     2461 tgtgaattac aggtgaccag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt
     2521 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     2581 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     2641 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     2701 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga attaaactat
     2761 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata
     2821 atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
     2881 aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg
     2941 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct
     3001 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta
     3061 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg
     3121 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc
     3181 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca
     3241 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg
     3301 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg
     3361 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca
     3421 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt
     3481 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt
     3541 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc
     3601 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt
     3661 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct
     3721 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag
     3781 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga
     3841 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac
     3901 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc
     3961 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
     4021 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
     4081 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
     4141 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
     4201 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
     4261 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
     4321 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
     4381 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
     4441 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
     4501 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
     4561 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
     4621 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
     4681 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
     4741 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
     4801 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
     4861 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
     4921 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
     4981 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
     5041 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
     5101 gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
     5161 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
     5221 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
     5281 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
     5341 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
     5401 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
     5461 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
     5521 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
     5581 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
     5641 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
     5701 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
     5761 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
     5821 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
     5881 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
     5941 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
     6001 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
     6061 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
     6121 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
     6181 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
     6241 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca
     6301 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
     6361 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
     6421 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
     6481 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
     6541 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa
     6601 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg
     6661 accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     6721 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     6781 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     6841 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     6901 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     6961 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7021 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7081 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7141 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7201 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7261 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7321 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7381 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7441 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7501 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7561 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     7621 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7681 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7741 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7801 aagggctgat gaatccccta atgattttta tcaaaatcat taagttaagg tagatacaca
     7861 tcttgtcata tgatcaaatg gtttcgccaa aaatcaataa tcagacaaca aaatgtgcga
     7921 actcgatatt ttacacgact ctctttacca attctgcccc gaattacact taaaacgact
     7981 caacagctta acgttggctt gccacgcctt acttgactgt aaaactctca ctcttaccga
     8041 acttggccgt aacctgccaa ccaaagcgag aacaaaacat aacatcaaac gaatcgaccg
     8101 attgttaggt aatcgtcacc tccacaaaga gcgactcgct gtataccgtt ggcatgctag
     8161 ctttatctgt tcgggcaata cgatgcccat tgtacttgtt gactggtctg atatccgtga
     8221 gcaaaaacgg cttatggtat tgcgagcttc agtcgcacta cacggtcgtt ctgttactct
     8281 ttatgagaaa gcgttcccgc tttcagagca atgttcaaag aaagctcatg accaatttct
     8341 agccgacctt gcgagcattc taccgagtaa caccacaccg ctcattgtca gtgatgctgg
     8401 ctttaaagtg ccatggtata aatccgttga gaagctgggt tggtactggt taagtcgagt
     8461 aagaggaaaa gtacaatatg cagacctagg agcggaaaac tggaaaccta tcagcaactt
     8521 acatgatatg tcatctagtc actcaaagac tttaggctat aagaggctga ctaaaagcaa
     8581 tccaatctca tgccaaattc tattgtataa atctcgctct aaaggccgaa aaaatcagcg
     8641 ctcgacacgg actcattgtc accacccgtc acctaaaatc tactcagcgt cggcaaagga
     8701 gccatggatt ctagcaacta acttacctgt tgaaattcga acacccaaac aacttgttaa
     8761 tatctattcg aagcgaatgc agattgaaga aaccttccga gacttgaaaa gtcctgccta
     8821 cggactaggc ctacgccata gccgaacgag cagctcagag cgttttgata tcatgctgct
     8881 aatcgccctg atgcttcaac taacatgttg gcttgcgggc gttcatgctc agaaacaagg
     8941 ttgggacaag cacttccagg ctaacacagt cagaaatcga aacgtactct caacagttcg
     9001 cttaggcatg gaagttttgc ggcattctgg ctacacaata acaagggaag actcactcgt
     9061 ggctgcaacc ctgcttactc aaaatctatt cacacatggt tacgttttgg ggaaattatg
     9121 aggggatctc tcagcgttaa gggattttgg tcatgcattc taggtactaa aacaattcat
     9181 ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa
     9241 atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa
     9301 tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc
     9361 catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt
     9421 cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt
     9481 cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg
     9541 ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga
     9601 gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact
     9661 cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc
     9721 gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct
     9781 tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata
     9841 ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt
     9901 ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca
     9961 tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa
    10021 caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc
    10081 tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa
    10141 accgcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg
    10201 cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt
    10261 aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat
    10321 gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg
    10381 gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca
    10441 ttgcggacgt ttttaatgta ctgaattaac gccgaattaa ttcctaggcc accatgttgg
    10501 gcccggcgcg ccaagcttgc atgcctgcag gtcaacatgg tggagcacga cactctcgtc
    10561 tactccaaga atatcaaaga tacagtctca gaagaccaga gggctattga gacttttcaa
    10621 caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc
    10681 gaaaggacag tagaaaagga agatggcttc tacaaatgcc atcattgcga taaaggaaag
    10741 gctatcgttc aagatgcctc taccgacagt ggtcccaaag atggaccccc acccacgagg
    10801 aacatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat
    10861 ggtcaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc
    10921 agaagaccag agggctat
//
