LOCUS       sequence-305822-       11579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding TRV2 complete genome with MCS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11579)
  AUTHORS   Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
            DF
  TITLE     Multiplexed heritable gene editing using RNA viruses and mobile 
            single guide RNAs.
  JOURNAL   Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y.
  PUBMED    32483329
REFERENCE   2  (bases 1 to 11579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y."
COMMENT     Sequence Label: pEE083
FEATURES             Location/Qualifiers
     source          1..11579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_RNA        1898..1973
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             2301..2606
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2485..2504
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             3083..3100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3135..3387
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3409..3433
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4734..5363
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5792..6865
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6931..7125
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7361..7383)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7469..7609
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7524..7543
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7624..7641)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7795..8383)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7875..7894)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9808..10602)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11027..11051
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ctctaccgac agtggtccca aagatggacc
      181 cccacccacg aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggataaaac attgcaccta
      361 tggtgttgcc ctggctgggg tatgtcagtg atcgcagtag aatgtactaa ttgacaagtt
      421 ggagaatacg gtagaacgtc cttatccaac acagccttta tccctctccc tgacgaggtt
      481 tttgtcagtg taatatttct ttttgaacta tccagcttag taccgtacgg gaaagtgact
      541 ggtgtgctta tctttgaaat gttactttgg gtttcggttc tttaggttag taagaaagca
      601 cttgtcttct catacaaagg aaaacctgag acgtatcgct tacgaaagta gcaatgaaag
      661 aaaggtggtg gttttaatcg ctaccgcaaa aacgatgggg tcgttttaat taacttctcc
      721 tacgcaagcg tctaaacgga cgttggggtt ttgctagttt ctttagagaa aactagctaa
      781 gtctttaatg ttatcattag agatggcata aatataatac ttgtgtctgc tgataagatc
      841 attttaattt ggacgattag acttgttgaa ctacaggtta ctgaatcact tgcgctaatc
      901 aacatgggag atatgtacga tgaatcattt gacaagtcgg gcggtcctgc tgacttgatg
      961 gacgattctt gggtggaatc agtttcgtgg aaagatctgt tgaagaagtt acacagcata
     1021 aaatttgcac tacagtctgg tagagatgag atcactgggt tactagcggc actgaataga
     1081 cagtgtcctt attcaccata tgagcagttt ccagataaga aggtgtattt ccttttagac
     1141 tcacgggcta acagtgctct tggtgtgatt cagaacgctt cagcgttcaa gagacgagct
     1201 gatgagaaga atgcagtggc gggtgttaca aatattcctg cgaatccaaa cacaacggtt
     1261 acgacgaacc aagggagtac tactactacc aaggcgaaca ctggctcgac tttggaagaa
     1321 gacttgtaca cttattacaa attcgatgat gcctctacag ctttccacaa atctctaact
     1381 tcgttagaga acatggagtt gaagagttat taccgaagga actttgagaa agtattcggg
     1441 attaagtttg gtggagcagc tgctagttca tctgcaccgc ctccagcgag tggaggtccg
     1501 atacgtccta atccctaggg atttaaggac gtgaactctg ttgagatctc tgtgaaattc
     1561 agagggtggg tgataccata ttcactgatg ccattagcga catctaaata gggctaattg
     1621 tgactaattt gagggaattt cctttaccat tgacgtcagt gtcgttggta gcatttgagt
     1681 ttcggagcat cttgttctgg ggtttcacac tatctttaga gaaagtgtta agttaattaa
     1741 gttatcttaa ttaagagcat aattatactg atttgtctct cgttgataga gtctatcatt
     1801 ctgttactaa aaatttgaca actcggtttg ctgacctact ggttactgta tcacttaccc
     1861 gagttaacga gtctagaaac ggcggcaggt gccatgggtt ttagagctag aaatagcaag
     1921 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcgtttaaa
     1981 caagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact
     2041 gatatgtata cccgaagtat gtcaaaagga ggtatgctat gaagcagcgt attacagtga
     2101 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct
     2161 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga
     2221 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga
     2281 cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt
     2341 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
     2401 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     2461 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggttt
     2521 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     2581 ttaacctgat gttctgggga atataacacc tgcgctcagt gccgggcatg tcccgaagac
     2641 attaaactac ggttctttaa gtagatccgt gtctgaagtt ttaggttcaa tttaaaccta
     2701 cgagattgac attctcgact gatcttgatt gatcggtaag tcttttgtaa tttaattttc
     2761 tttttgattt tattttaaat tgttatctgt ttctgtgtat agactgtttg agatcggcgt
     2821 ttggccgact cattgtctta ccatagggga acggactttg tttgtgttgt tattttattt
     2881 gtattttatt aaaattctca acgatctgaa aaagcctcgc ggctaagaga ttgttggggg
     2941 gtgagtaagt acttttaaag tgatgatggt tacaaaggca aaaggggtaa aacccctcgc
     3001 ctacgtaagc gttattacgc ccgtctgtac ttatatcagt acactgacga gtccctaaag
     3061 gacgaaacgg gagaacgcta gccaccacca ccaccaccac gtgtgaatta caggtgacca
     3121 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     3181 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     3241 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     3301 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     3361 gcgcggtgtc atctatgtta ctagatcggg aattaaacta tcagtgtttg acaggatata
     3421 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     3481 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     3541 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     3601 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     3661 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     3721 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     3781 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     3841 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     3901 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     3961 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     4021 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     4081 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     4141 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     4201 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     4261 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     4321 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     4381 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     4441 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     4501 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
     4561 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     4621 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     4681 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     4741 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     4801 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     4861 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     4921 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     4981 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     5041 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     5101 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     5161 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     5221 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     5281 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     5341 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     5401 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     5461 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     5521 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     5581 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     5641 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     5701 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg
     5761 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
     5821 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
     5881 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
     5941 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
     6001 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
     6061 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
     6121 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
     6181 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
     6241 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
     6301 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
     6361 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
     6421 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
     6481 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
     6541 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
     6601 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
     6661 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
     6721 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
     6781 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
     6841 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
     6901 aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa
     6961 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
     7021 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
     7081 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
     7141 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
     7201 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
     7261 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca
     7321 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     7381 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     7441 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     7501 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     7561 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     7621 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7681 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7741 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7801 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7861 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7921 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7981 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8041 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8101 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8161 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8221 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8281 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8341 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8401 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggctga tgaatcccct
     8461 aatgattttt atcaaaatca ttaagttaag gtagatacac atcttgtcat atgatcaaat
     8521 ggtttcgcca aaaatcaata atcagacaac aaaatgtgcg aactcgatat tttacacgac
     8581 tctctttacc aattctgccc cgaattacac ttaaaacgac tcaacagctt aacgttggct
     8641 tgccacgcct tacttgactg taaaactctc actcttaccg aacttggccg taacctgcca
     8701 accaaagcga gaacaaaaca taacatcaaa cgaatcgacc gattgttagg taatcgtcac
     8761 ctccacaaag agcgactcgc tgtataccgt tggcatgcta gctttatctg ttcgggcaat
     8821 acgatgccca ttgtacttgt tgactggtct gatatccgtg agcaaaaacg gcttatggta
     8881 ttgcgagctt cagtcgcact acacggtcgt tctgttactc tttatgagaa agcgttcccg
     8941 ctttcagagc aatgttcaaa gaaagctcat gaccaatttc tagccgacct tgcgagcatt
     9001 ctaccgagta acaccacacc gctcattgtc agtgatgctg gctttaaagt gccatggtat
     9061 aaatccgttg agaagctggg ttggtactgg ttaagtcgag taagaggaaa agtacaatat
     9121 gcagacctag gagcggaaaa ctggaaacct atcagcaact tacatgatat gtcatctagt
     9181 cactcaaaga ctttaggcta taagaggctg actaaaagca atccaatctc atgccaaatt
     9241 ctattgtata aatctcgctc taaaggccga aaaaatcagc gctcgacacg gactcattgt
     9301 caccacccgt cacctaaaat ctactcagcg tcggcaaagg agccatggat tctagcaact
     9361 aacttacctg ttgaaattcg aacacccaaa caacttgtta atatctattc gaagcgaatg
     9421 cagattgaag aaaccttccg agacttgaaa agtcctgcct acggactagg cctacgccat
     9481 agccgaacga gcagctcaga gcgttttgat atcatgctgc taatcgccct gatgcttcaa
     9541 ctaacatgtt ggcttgcggg cgttcatgct cagaaacaag gttgggacaa gcacttccag
     9601 gctaacacag tcagaaatcg aaacgtactc tcaacagttc gcttaggcat ggaagttttg
     9661 cggcattctg gctacacaat aacaagggaa gactcactcg tggctgcaac cctgcttact
     9721 caaaatctat tcacacatgg ttacgttttg gggaaattat gaggggatct ctcagcgtta
     9781 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
     9841 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
     9901 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
     9961 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
    10021 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
    10081 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
    10141 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
    10201 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
    10261 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
    10321 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
    10381 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
    10441 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
    10501 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
    10561 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
    10621 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
    10681 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
    10741 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
    10801 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
    10861 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
    10921 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
    10981 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
    11041 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
    11101 actgaattaa cgccgaatta attcctaggc caccatgttg ggcccggcgc gccaagcttg
    11161 catgcctgca ggtcaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag
    11221 atacagtctc agaagaccag agggctattg agacttttca acaaagggta atatcgggaa
    11281 acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca gtagaaaagg
    11341 aagatggctt ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct
    11401 ctaccgacag tggtcccaaa gatggacccc cacccacgag gaacatcgtg gaaaaagaag
    11461 acgttccaac cacgtcttca aagcaagtgg attgatgtga tggtcaacat ggtggagcac
    11521 gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca gagggctat
//
