LOCUS       sequence-305826-       11610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding TRV2 with tRNA multiplexed truncated FT augmented 
            gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11610)
  AUTHORS   Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
            DF
  TITLE     Multiplexed heritable gene editing using RNA viruses and mobile 
            single guide RNAs.
  JOURNAL   Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y.
  PUBMED    32483329
REFERENCE   2  (bases 1 to 11610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y."
COMMENT     Sequence Label: pEE491
FEATURES             Location/Qualifiers
     source          1..11610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        427..772
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     715..735
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_RNA        2327..2402
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        2610..2685
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        2893..2968
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             3540..3557
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3592..3844
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3866..3890
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5191..5820
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6249..7322
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7388..7582
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7818..7840)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7926..8066
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7981..8000
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8081..8098)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8252..8840)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8332..8351)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10265..11059)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11484..11508
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
      781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
      841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
      901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
      961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
     1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
     1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
     1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
     1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
     1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
     1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
     1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
     1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
     1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
     1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
     1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
     1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
     1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
     1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
     1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
     1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
     1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
     2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
     2101 ttgagtttcg gagcatcttg ttctggggtt tcacactatc tttagagaaa gtgttaagtt
     2161 aattaagtta tcttaattaa gagcataatt atactgattt gtctctcgtt gatagagtct
     2221 atcattctgt tactaaaaat ttgacaactc ggtttgctga cctactggtt actgtatcac
     2281 ttacccgagt taacgagtct agaaacgtgt gaaagaaaca attgaggttt tagagctaga
     2341 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     2401 gctccctagt ctataaatat aagagaccct cttatagtaa gcagagttgt tggagacgtt
     2461 cttgatccgt ttaatagatc aatcactcta aaggttactt atggccaaaa caaagcacca
     2521 gtggtctagt ggtagaatag taccctgcca cggtacagac ccgggttcga ttcccggctg
     2581 gtgcagtgct tggtagtagc gactccatgg ttttagagct agaaatagca agttaaaata
     2641 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgctccct agtctataaa
     2701 tataagagac cctcttatag taagcagagt tgttggagac gttcttgatc cgtttaatag
     2761 atcaatcact ctaaaggtta cttatggcca aaacaaagca ccagtggtct agtggtagaa
     2821 tagtaccctg ccacggtaca gacccgggtt cgattcccgg ctggtgcaca ggttgattgt
     2881 cttctcaagc aggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     2941 acttgaaaaa gtggcaccga gtcggtgctc cctagtctat aaatataaga gaccctctta
     3001 tagtaagcag agttgttgga gacgttcttg atccgtttaa tagatcaatc actctaaagg
     3061 ttacttatgg ccaaagtgcc gggcatgtcc cgaagacatt aaactacggt tctttaagta
     3121 gatccgtgtc tgaagtttta ggttcaattt aaacctacga gattgacatt ctcgactgat
     3181 cttgattgat cggtaagtct tttgtaattt aattttcttt ttgattttat tttaaattgt
     3241 tatctgtttc tgtgtataga ctgtttgaga tcggcgtttg gccgactcat tgtcttacca
     3301 taggggaacg gactttgttt gtgttgttat tttatttgta ttttattaaa attctcaacg
     3361 atctgaaaaa gcctcgcggc taagagattg ttggggggtg agtaagtact tttaaagtga
     3421 tgatggttac aaaggcaaaa ggggtaaaac ccctcgccta cgtaagcgtt attacgcccg
     3481 tctgtactta tatcagtaca ctgacgagtc cctaaaggac gaaacgggag aacgctagcc
     3541 accaccacca ccaccacgtg tgaattacag gtgaccagct cgaatttccc cgatcgttca
     3601 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
     3661 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
     3721 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
     3781 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
     3841 gatcgggaat taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
     3901 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
     3961 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac
     4021 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg
     4081 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt
     4141 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt
     4201 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg
     4261 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt
     4321 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc
     4381 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg
     4441 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag
     4501 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca
     4561 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca
     4621 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg
     4681 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg
     4741 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg
     4801 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg
     4861 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc
     4921 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc
     4981 gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa
     5041 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag
     5101 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg
     5161 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa
     5221 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg
     5281 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg
     5341 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg
     5401 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg
     5461 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac
     5521 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat
     5581 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt
     5641 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag
     5701 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag
     5761 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga
     5821 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga
     5881 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc
     5941 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc
     6001 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga
     6061 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa
     6121 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca
     6181 ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag
     6241 gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg
     6301 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag
     6361 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc
     6421 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag
     6481 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg
     6541 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
     6601 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
     6661 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
     6721 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
     6781 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
     6841 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
     6901 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
     6961 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga
     7021 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
     7081 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
     7141 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
     7201 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
     7261 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
     7321 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc
     7381 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc
     7441 cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata
     7501 taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta
     7561 aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag
     7621 cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg
     7681 ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag
     7741 ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc
     7801 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     7861 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     7921 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     7981 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     8041 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     8101 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8161 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8221 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8281 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8341 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8401 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8461 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8521 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8581 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8641 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     8701 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8761 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8821 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8881 ggaacgaaaa ctcacgttaa gggctgatga atcccctaat gatttttatc aaaatcatta
     8941 agttaaggta gatacacatc ttgtcatatg atcaaatggt ttcgccaaaa atcaataatc
     9001 agacaacaaa atgtgcgaac tcgatatttt acacgactct ctttaccaat tctgccccga
     9061 attacactta aaacgactca acagcttaac gttggcttgc cacgccttac ttgactgtaa
     9121 aactctcact cttaccgaac ttggccgtaa cctgccaacc aaagcgagaa caaaacataa
     9181 catcaaacga atcgaccgat tgttaggtaa tcgtcacctc cacaaagagc gactcgctgt
     9241 ataccgttgg catgctagct ttatctgttc gggcaatacg atgcccattg tacttgttga
     9301 ctggtctgat atccgtgagc aaaaacggct tatggtattg cgagcttcag tcgcactaca
     9361 cggtcgttct gttactcttt atgagaaagc gttcccgctt tcagagcaat gttcaaagaa
     9421 agctcatgac caatttctag ccgaccttgc gagcattcta ccgagtaaca ccacaccgct
     9481 cattgtcagt gatgctggct ttaaagtgcc atggtataaa tccgttgaga agctgggttg
     9541 gtactggtta agtcgagtaa gaggaaaagt acaatatgca gacctaggag cggaaaactg
     9601 gaaacctatc agcaacttac atgatatgtc atctagtcac tcaaagactt taggctataa
     9661 gaggctgact aaaagcaatc caatctcatg ccaaattcta ttgtataaat ctcgctctaa
     9721 aggccgaaaa aatcagcgct cgacacggac tcattgtcac cacccgtcac ctaaaatcta
     9781 ctcagcgtcg gcaaaggagc catggattct agcaactaac ttacctgttg aaattcgaac
     9841 acccaaacaa cttgttaata tctattcgaa gcgaatgcag attgaagaaa ccttccgaga
     9901 cttgaaaagt cctgcctacg gactaggcct acgccatagc cgaacgagca gctcagagcg
     9961 ttttgatatc atgctgctaa tcgccctgat gcttcaacta acatgttggc ttgcgggcgt
    10021 tcatgctcag aaacaaggtt gggacaagca cttccaggct aacacagtca gaaatcgaaa
    10081 cgtactctca acagttcgct taggcatgga agttttgcgg cattctggct acacaataac
    10141 aagggaagac tcactcgtgg ctgcaaccct gcttactcaa aatctattca cacatggtta
    10201 cgttttgggg aaattatgag gggatctctc agcgttaagg gattttggtc atgcattcta
    10261 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
    10321 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
    10381 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
    10441 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
    10501 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
    10561 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
    10621 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
    10681 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
    10741 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
    10801 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
    10861 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
    10921 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
    10981 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
    11041 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
    11101 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
    11161 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
    11221 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
    11281 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
    11341 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
    11401 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
    11461 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
    11521 agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt
    11581 cctaggccac catgttgggc ccggcgcgcc
//
