LOCUS       sequence-305838-        4026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Olfr644 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Inagaki S, Iwata R, Iwamoto M, Imai T
  TITLE     Widespread Inhibition, Antagonism, and Synergy in Mouse Olfactory 
            Sensory Neurons In Vivo.
  JOURNAL   Cell Rep. 2020 Jun 30;31(13):107814. doi: 
            10.1016/j.celrep.2020.107814.
  PUBMED    32610120
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-06-30"; volume: "31(13):107814. doi"; pages: " 
            10.1016/j.celrep.2020.107814"
COMMENT     Sequence Label: pME18S-F-R-Olfr644
FEATURES             Location/Qualifiers
     source          1..4026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             790..813
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             835..873
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
     polyA_signal    1914..2048
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1951..1970)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2005..2024
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2087..2104)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2258..2846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2338..2357)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3017..3808,3809..3877))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3640..3659
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3878..3982)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(4022..4026,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attgccatgg actacaagga cgacgacgac aaggaattca tgaacgggac cgagggccca
      841 aacttctacg tgcctttctc caacaagacg ggcgtggtgc tcgagatggg aggtgaagcc
      901 cacaacagct ctgggttgcc tcccttcatc ttgacaggac tgccagggat ggagacctcc
      961 cagcactggt tgttcctgct ccttggtgtt ctttacagcg tctccatagt gggcaatgcc
     1021 ctgattctgt tcatcatcaa ggaggaagag agtcttcacc agcccatgta ctacttccta
     1081 tctctgctgt caggaaatga ccttggtgtg tctttctcca cactgcctac agttctggga
     1141 gtgttttgtt ttcacttaag agagatcagt tttaactcct gcatgtccca aatgttcttt
     1201 atccatcttt tctctttcat ggagtctggg attctgctgg ccatgagctt tgatcgctat
     1261 gtagccatct gtaacccact gcgctattcc acagtactca ctgatgcccg agtgatgtgg
     1321 atgggtgtat gtgttttcct tcgaagtttc tgcatgattt tcccattacc tttccttctg
     1381 aagaggttgc ccttctgcaa ggctaatgtg ctctctcatg cctactgtct gcatcctgat
     1441 ctgattcgac tgccttgtgg tgacacttcc attaataata tatttggtct gtccattgtc
     1501 atctctacct ttgggctgga ttctgcactc attttccttt cttatgttct catacttcgc
     1561 tcagtgcttg ctattgcctc ccgggaagaa aggatgaaaa cactgaatac atgtgtgtca
     1621 cacctgtgtg ctgtgctcat cttctatgtg cctaaggtag gtgtgtccat gtttgctcgc
     1681 tatgggaggc atgcccctca ctatgtacac acactcttgt cactcatcta tctctttgtt
     1741 cctccaatgc tcaaccctgt catctactcc atcaaaacca aggagattcg tcggaggttt
     1801 tgcaaaatcc tgctggggaa caaattttga ccgcggaatc actagtctag agaaaaaacc
     1861 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     1921 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1981 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     2041 tctggatccc cgggtaccga gctcgaatta attcctcttc cgcttcctcg ctcactgact
     2101 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     2161 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2221 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2281 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2341 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2401 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2461 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2521 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2581 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2641 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     2701 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2761 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2821 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2881 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2941 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3001 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3061 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3121 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3181 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3241 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3301 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3361 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3421 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3481 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3541 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3601 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3661 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3721 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3781 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3841 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3901 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3961 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgca agcttggctg
     4021 tggaat
//
