LOCUS       sequence-305848-       11044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11044)
  AUTHORS   Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
            DF
  TITLE     Multiplexed heritable gene editing using RNA viruses and mobile 
            single guide RNAs.
  JOURNAL   Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y.
  PUBMED    32483329
REFERENCE   2  (bases 1 to 11044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y."
COMMENT     Sequence Label: pEE385
FEATURES             Location/Qualifiers
     source          1..11044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        427..772
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     715..735
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_RNA        2327..2402
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             2974..2991
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3026..3278
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3300..3324
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4625..5254
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5683..6756
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6822..7016
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7252..7274)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7360..7500
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7415..7434
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7515..7532)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7686..8274)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7766..7785)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9699..10493)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10918..10942
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
      781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
      841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
      901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
      961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
     1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
     1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
     1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
     1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
     1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
     1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
     1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
     1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
     1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
     1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
     1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
     1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
     1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
     1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
     1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
     1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
     1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
     2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
     2101 ttgagtttcg gagcatcttg ttctggggtt tcacactatc tttagagaaa gtgttaagtt
     2161 aattaagtta tcttaattaa gagcataatt atactgattt gtctctcgtt gatagagtct
     2221 atcattctgt tactaaaaat ttgacaactc ggtttgctga cctactggtt actgtatcac
     2281 ttacccgagt taacgagtct agaaacttga ttgtcttctc aagcaggttt tagagctaga
     2341 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     2401 gctccctagt ctataaatat aagagaccct cttatagtaa gcagagttgt tggagacgtt
     2461 cttgatccgt ttaatagatc aatcactcta aaggttactt atggccaaag tgccgggcat
     2521 gtcccgaaga cattaaacta cggttcttta agtagatccg tgtctgaagt tttaggttca
     2581 atttaaacct acgagattga cattctcgac tgatcttgat tgatcggtaa gtcttttgta
     2641 atttaatttt ctttttgatt ttattttaaa ttgttatctg tttctgtgta tagactgttt
     2701 gagatcggcg tttggccgac tcattgtctt accatagggg aacggacttt gtttgtgttg
     2761 ttattttatt tgtattttat taaaattctc aacgatctga aaaagcctcg cggctaagag
     2821 attgttgggg ggtgagtaag tacttttaaa gtgatgatgg ttacaaaggc aaaaggggta
     2881 aaacccctcg cctacgtaag cgttattacg cccgtctgta cttatatcag tacactgacg
     2941 agtccctaaa ggacgaaacg ggagaacgct agccaccacc accaccacca cgtgtgaatt
     3001 acaggtgacc agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag
     3061 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     3121 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     3181 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     3241 taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattaaact atcagtgttt
     3301 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
     3361 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
     3421 ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc
     3481 ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg
     3541 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata
     3601 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct
     3661 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc
     3721 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc
     3781 gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg
     3841 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc
     3901 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg
     3961 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc
     4021 atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc
     4081 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc
     4141 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca
     4201 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag
     4261 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa
     4321 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg
     4381 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc
     4441 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg
     4501 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
     4561 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga
     4621 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc
     4681 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
     4741 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
     4801 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
     4861 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
     4921 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt
     4981 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
     5041 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
     5101 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
     5161 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
     5221 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
     5281 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
     5341 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
     5401 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
     5461 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
     5521 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
     5581 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgcctgccg
     5641 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca
     5701 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc
     5761 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
     5821 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg
     5881 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
     5941 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
     6001 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
     6061 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc
     6121 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
     6181 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
     6241 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
     6301 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
     6361 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
     6421 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc
     6481 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc
     6541 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc
     6601 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg
     6661 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc
     6721 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
     6781 attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt
     6841 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
     6901 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
     6961 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
     7021 tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc
     7081 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc
     7141 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg
     7201 cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     7261 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     7321 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     7381 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     7441 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     7501 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7561 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7621 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7681 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7741 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7801 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7861 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7921 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7981 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8041 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8101 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     8161 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8221 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8281 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggctg
     8341 atgaatcccc taatgatttt tatcaaaatc attaagttaa ggtagataca catcttgtca
     8401 tatgatcaaa tggtttcgcc aaaaatcaat aatcagacaa caaaatgtgc gaactcgata
     8461 ttttacacga ctctctttac caattctgcc ccgaattaca cttaaaacga ctcaacagct
     8521 taacgttggc ttgccacgcc ttacttgact gtaaaactct cactcttacc gaacttggcc
     8581 gtaacctgcc aaccaaagcg agaacaaaac ataacatcaa acgaatcgac cgattgttag
     8641 gtaatcgtca cctccacaaa gagcgactcg ctgtataccg ttggcatgct agctttatct
     8701 gttcgggcaa tacgatgccc attgtacttg ttgactggtc tgatatccgt gagcaaaaac
     8761 ggcttatggt attgcgagct tcagtcgcac tacacggtcg ttctgttact ctttatgaga
     8821 aagcgttccc gctttcagag caatgttcaa agaaagctca tgaccaattt ctagccgacc
     8881 ttgcgagcat tctaccgagt aacaccacac cgctcattgt cagtgatgct ggctttaaag
     8941 tgccatggta taaatccgtt gagaagctgg gttggtactg gttaagtcga gtaagaggaa
     9001 aagtacaata tgcagaccta ggagcggaaa actggaaacc tatcagcaac ttacatgata
     9061 tgtcatctag tcactcaaag actttaggct ataagaggct gactaaaagc aatccaatct
     9121 catgccaaat tctattgtat aaatctcgct ctaaaggccg aaaaaatcag cgctcgacac
     9181 ggactcattg tcaccacccg tcacctaaaa tctactcagc gtcggcaaag gagccatgga
     9241 ttctagcaac taacttacct gttgaaattc gaacacccaa acaacttgtt aatatctatt
     9301 cgaagcgaat gcagattgaa gaaaccttcc gagacttgaa aagtcctgcc tacggactag
     9361 gcctacgcca tagccgaacg agcagctcag agcgttttga tatcatgctg ctaatcgccc
     9421 tgatgcttca actaacatgt tggcttgcgg gcgttcatgc tcagaaacaa ggttgggaca
     9481 agcacttcca ggctaacaca gtcagaaatc gaaacgtact ctcaacagtt cgcttaggca
     9541 tggaagtttt gcggcattct ggctacacaa taacaaggga agactcactc gtggctgcaa
     9601 ccctgcttac tcaaaatcta ttcacacatg gttacgtttt ggggaaatta tgaggggatc
     9661 tctcagcgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa
     9721 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
     9781 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
     9841 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
     9901 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
     9961 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
    10021 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
    10081 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
    10141 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
    10201 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
    10261 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
    10321 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
    10381 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
    10441 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
    10501 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
    10561 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
    10621 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt
    10681 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
    10741 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
    10801 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
    10861 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
    10921 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac
    10981 gtttttaatg tactgaatta acgccgaatt aattcctagg ccaccatgtt gggcccggcg
    11041 cgcc
//
