LOCUS       sequence-305855-       10829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express YE1-BE3-FNLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Zuo E, Sun Y, Yuan T, He B, Zhou C, Ying W, Liu J, Wei W, Zeng R, Li
            Y, Yang H
  TITLE     A rationally engineered cytosine base editor retains high on-target 
            activity while reducing both DNA and RNA off-target effects.
  JOURNAL   Nat Methods. 2020 May 18. pii: 10.1038/s41592-020-0832-x. doi: 
            10.1038/s41592-020-0832-x.
  PUBMED    32424272
REFERENCE   2  (bases 1 to 10829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2020 May 18. pii: 10.1038/s41592-020-0832-x. doi: 
            10.1038/s41592-020-0832-x."
COMMENT     Sequence Label: CMV-YE1-BE3-FNLS-CMV-mCherry
FEATURES             Location/Qualifiers
     source          1..10829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     246..265
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        246..264
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      271..280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             280..345
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             352..372
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1132..1155,1156..1158,1159..5232)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1132..5232
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5245..5493
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5506..5526
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5566..5790
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5861..5877)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5861..5877)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5874..5896)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5885..5901
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5909..5915,5916..5933,5934..5939))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5954..5975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6092..6109)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6263..6851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6343..6362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7022..7813,7814..7882))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7645..7664
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7883..7987)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8066..8085)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8318..8621
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8622..8825
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      8853..8862
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8859..9569
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9004..9022)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9272..9291
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    9570..10158
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9623..9643)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10041..10052)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    10200..10424
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        10450..10829
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg
      241 gccgctaata cgactcacta taggggatcc gccaccatgg actataagga ccacgacgga
      301 gactacaagg atcatgatat tgattacaaa gacgatgacg ataagatggc cccaaagaag
      361 aagcggaagg tcggtatcca cggagtccca gcagccatga gctcagagac tggcccagtg
      421 gctgtggacc ccacattgag acggcggatc gagccccatg agtttgaggt attcttcgat
      481 ccgagagagc tccgcaagga gacctgcctg ctttacgaaa ttaattgggg gggccggcac
      541 tccatttggc gacatacatc acagaacact aacaagcacg tcgaagtcaa cttcatcgag
      601 aagttcacga cagaaagata tttctgtccg aacacaaggt gcagcattac ctggtttctc
      661 agctacagcc catgcggcga atgtagtagg gccatcactg aattcctgtc aaggtatccc
      721 cacgtcactc tgtttattta catcgcaagg ctgtaccacc acgctgaccc cgaaaatcga
      781 caaggcctgc gggatttgat ctcttcaggt gtgactatcc aaattatgac tgagcaggag
      841 tcaggatact gctggagaaa ctttgtgaat tatagcccga gtaatgaagc ccactggcct
      901 aggtatcccc atctgtgggt acgactgtac gttcttgaac tgtactgcat catactgggc
      961 ctgcctcctt gtctcaacat tctgagaagg aagcagccac agctgacatt ctttaccatc
     1021 gctcttcagt cttgtcatta ccagcgactg cccccacaca ttctctgggc caccgggttg
     1081 aaaagcggca gcgagactcc cgggacctca gagtccgcca cacccgaaag tgacaagaag
     1141 tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag
     1201 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
     1261 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
     1321 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
     1381 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc
     1441 ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac
     1501 gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac
     1561 agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc
     1621 cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg
     1681 ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc
     1741 ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat
     1801 ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggcaacct gattgccctg
     1861 agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg
     1921 cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac
     1981 cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac
     2041 atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga
     2101 tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct
     2161 gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac
     2221 ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac
     2281 ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc
     2341 ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg
     2401 cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg
     2461 accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg
     2521 atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag
     2581 ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac
     2641 gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg
     2701 accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag
     2761 aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg
     2821 aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa
     2881 gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag
     2941 gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca
     3001 ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac
     3061 gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg
     3121 aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag
     3181 tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt
     3241 aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt
     3301 gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg
     3361 gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc
     3421 agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc
     3481 gaagagggca tcaaagagct gggcagccag atcctgaaag aacacccagt ggaaaacacc
     3541 cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg
     3601 gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag
     3661 agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg
     3721 ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg
     3781 cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag
     3841 agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc
     3901 cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac
     3961 gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc
     4021 gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc
     4081 cacgacgcct acctgaacgc cgtcgtggga accgccctaa tcaaaaagta ccctaagctg
     4141 gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag
     4201 agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac
     4261 tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag
     4321 acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg
     4381 aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc
     4441 ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag
     4501 gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg
     4561 gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg
     4621 gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc
     4681 aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc
     4741 gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac
     4801 gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag
     4861 ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac
     4921 tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac
     4981 gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag
     5041 caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc
     5101 aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac
     5161 gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag
     5221 ctgggaggcg attcaggcgg atctactaat ctgtcagata ttattgaaaa ggagaccggt
     5281 aagcaactgg ttatccagga atccatcctc atgctcccag aggaggtgga agaagtcatt
     5341 gggaacaagc cggaaagcga tatactcgtg cacaccgcct acgacgagag caccgacgag
     5401 aatgtcatgc ttctgactag cgacgcccct gaatacaagc cttgggctct ggtcatacag
     5461 gatagcaacg gtgagaacaa gattaagatg ctctctggtg gttctcccaa gaagaagagg
     5521 aaagtcgcta gcggcagcgg ctaaaaaccc gctgatcagc ctcgactgtg ccttctagtt
     5581 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     5641 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     5701 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     5761 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     5821 cgataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     5881 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     5941 cctagggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     6001 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     6061 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6121 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6181 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6241 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6301 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6361 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6421 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6481 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6541 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6601 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6661 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     6721 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6781 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6841 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6901 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6961 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7021 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7081 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7141 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7201 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7261 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7321 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7381 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7441 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7501 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7561 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7621 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7681 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7741 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7801 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7861 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7921 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7981 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatcgatctc
     8041 ccgatcccct agggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     8101 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     8161 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     8221 cgctgcttcg cgatgtacgg gccagatata ggtacccatt aattaaatag taatcaatta
     8281 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     8341 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     8401 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     8461 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
     8521 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
     8581 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     8641 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     8701 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
     8761 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     8821 gaggtcgttt agtgaaccgt cagatcacgc gtgccaccat ggtgagcaag ggcgaggagg
     8881 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     8941 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     9001 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     9061 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     9121 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     9181 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     9241 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     9301 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     9361 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     9421 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     9481 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     9541 ccggcggcat ggacgagctg tacaagtaaa atcaacctct ggattacaaa atttgtgaaa
     9601 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     9661 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     9721 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     9781 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     9841 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     9901 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     9961 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
    10021 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
    10081 tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc
    10141 tttgggccgc ctccccgcca ccggttaggg gcccgtttaa acccgctgat cagcctcgac
    10201 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
    10261 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
    10321 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
    10381 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcggcgc gcctaaaagt
    10441 tttgttactg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
    10501 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
    10561 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
    10621 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
    10681 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
    10741 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
    10801 tctacgtatt agtcatcgct attaccatg
//
