LOCUS       sequence-305894-       11715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing base editor nCDA1-VQRBE3 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   Tan J, Zhang F, Karcher D, Bock R
  TITLE     Expanding the genome-targeting scope and the site selectivity of 
            high-precision base editors.
  JOURNAL   Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.
  PUBMED    32005820
REFERENCE   2  (bases 1 to 11715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-31"; volume: "11(1):629. doi"; pages: " 
            10.1038/s41467-020-14465-z"
COMMENT     Sequence Label: pJT121_GalL_nCDA1-VQRBE3
FEATURES             Location/Qualifiers
     source          1..11715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1439..1462
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     1483..1499
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1483..1499
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             1513..2136
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     CDS             2185..6288
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6301..6549
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6562..6582
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6598..6845
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6614..6632)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(6863..6882)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6864..6882)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6892..6909)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6892..6908)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6901..6923)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7049..7504
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7136..7155)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7346..7367
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7805..8212
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             8213..9307
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(9801..9820)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9920..9942
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9980..9998)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    10040..10543
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        10580..10684
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10685..10753,10754..11545)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10903..10922)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcgt gacagccctc cgaaggaaga ctctcctccg
     1081 tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga tgtgcctcgc gccgcactgc
     1141 tccgaacaat aaagattcta caatactagc ttttatggtt atgaagagga aaaattggca
     1201 gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat taacaaccat aggatgataa
     1261 tgcgattagt tttttagcct tatttctggg gtaattaatc agcgaagcga tgatttttga
     1321 tctattaacg gatatataaa tgcaaaaact gcataaccac tttaactaat actttcaaca
     1381 ttttcggttt gtattacttc ttattcaaat gtaataaaag tatcaacaaa aaattgttaa
     1441 tatacctcta tactttaacg tcaaggagaa aaaaccccgg attctagaac tagtggatcc
     1501 cccgggaaaa aaatgaccga cgctgagtac gtgagaatcc atgagaagtt ggacatctac
     1561 acgtttaaga aacagttttt caacaacaaa aaatccgtgt cgcatagatg ctacgttctc
     1621 tttgaattaa aacgacgggg tgaacgtaga gcgtgttttt ggggctatgc tgtgaataaa
     1681 ccacagagcg ggacagaacg tggcattcac gccgaaatct ttagcattag aaaagtcgaa
     1741 gaatacctgc gcgacaaccc cggacaattc acgataaatt ggtactcatc ctggagtcct
     1801 tgtgcagatt gcgctgaaaa gatcttagaa tggtataacc aggagctgcg ggggaacggc
     1861 cacactttga aaatctgggc ttgcaaactc tattacgaga aaaatgcgag gaatcaaatt
     1921 gggctgtgga acctcagaga taacggggtt gggttgaatg taatggtaag tgaacactac
     1981 caatgttgca ggaaaatatt catccaatcg tcgcacaatc aattgaatga gaatagatgg
     2041 cttgagaaga ctttgaagcg agctgaaaaa cgacggagcg agttgtccat tatgattcag
     2101 gtaaaaatac tccacaccac taagagtcct gctgtttctg gttctgaaac tccaggtact
     2161 tctgaatctg ctactccaga atctatggac aagaagtact ccattgggct cgctatcggc
     2221 acaaacagcg tcggttgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc
     2281 aaagttctgg gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg
     2341 ttcgactccg gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat
     2401 acccgcagaa agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag
     2461 gtggatgact ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag
     2521 cacgagcgcc acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac
     2581 ccaaccatat atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg
     2641 ttgatctatc tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg
     2701 gacctgaacc cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac
     2761 aatcagcttt tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg
     2821 agcgctaggc tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag
     2881 aagaagaacg gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt
     2941 aaatctaact tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat
     3001 gatgatctcg acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg
     3061 gcaaagaacc tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc
     3121 accaaagctc cgctgagcgc tagtatgatc aagcgctatg atgagcacca ccaagacttg
     3181 actttgctga aggcccttgt cagacagcaa ctgcctgaga agtacaagga aattttcttc
     3241 gatcagtcta aaaatggcta cgccggatac attgacggcg gagcaagcca ggaggaattt
     3301 tacaaattta ttaagcccat cttggaaaaa atggacggca ccgaggagct gctggtaaag
     3361 cttaacagag aagatctgtt gcgcaaacag cgcactttcg acaatggaag catcccccac
     3421 cagattcacc tgggcgaact gcacgctatc ctcaggcggc aagaggattt ctaccccttt
     3481 ttgaaagata acagggaaaa gattgagaaa atcctcacat ttcggatacc ctactatgta
     3541 ggccccctcg cccggggaaa ttccagattc gcgtggatga ctcgcaaatc agaagagacc
     3601 atcactccct ggaacttcga ggaagtcgtg gataaggggg cctctgccca gtccttcatc
     3661 gaaaggatga ctaactttga taaaaatctg cctaacgaaa aggtgcttcc taaacactct
     3721 ctgctgtacg agtacttcac agtttataac gagctcacca aggtcaaata cgtcacagaa
     3781 gggatgagaa agccagcatt cctgtctgga gagcagaaga aagctatcgt ggacctcctc
     3841 ttcaagacga accggaaagt taccgtgaaa cagctcaaag aagactattt caaaaagatt
     3901 gaatgtttcg actctgttga aatcagcgga gtggaggatc gcttcaacgc atccctggga
     3961 acgtatcacg atctcctgaa aatcattaaa gacaaggact tcctggacaa tgaggagaac
     4021 gaggacattc ttgaggacat tgtcctcacc cttacgttgt ttgaagatag ggagatgatt
     4081 gaagaacgct tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa acagctcaag
     4141 aggcgccgat atacaggatg ggggcggctg tcaagaaaac tgatcaatgg gatccgagac
     4201 aagcagagtg gaaagacaat cctggatttt cttaagtccg atggatttgc caaccggaac
     4261 ttcatgcagt tgatccatga tgactctctc acctttaagg aggacatcca gaaagcacaa
     4321 gtttctggcc agggggacag tcttcacgag cacatcgcta atcttgcagg tagcccagct
     4381 atcaaaaagg gaatactgca gaccgttaag gtcgtggatg aactcgtcaa agtaatggga
     4441 aggcataagc ccgagaatat cgttatcgag atggcccgag agaaccaaac tacccagaag
     4501 ggacagaaga acagtaggga aaggatgaag aggattgaag agggtataaa agaactgggg
     4561 tcccaaatcc ttaaggaaca cccagttgaa aacacccagc ttcagaatga gaagctctac
     4621 ctgtactacc tgcagaacgg cagggacatg tacgtggatc aggaactgga catcaatcgg
     4681 ctctccgact acgacgtgga tcatatcgtg ccccagtctt ttctcaaaga tgattctatt
     4741 gataataaag tgttgacaag atccgataaa aatagaggga agagtgataa cgtcccctca
     4801 gaagaagttg tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc caaactgatc
     4861 acacaacgga agttcgataa tctgactaag gctgaacgag gtggcctgtc tgagttggat
     4921 aaagccggct tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc
     4981 caaattctcg attcacgcat gaacaccaag tacgatgaaa atgacaaact gattcgagag
     5041 gtgaaagtta ttactctgaa gtctaagctg gtctcagatt tcagaaagga ctttcagttt
     5101 tataaggtga gagagatcaa caattaccac catgcgcatg atgcctacct gaatgcagtg
     5161 gtaggcactg cacttatcaa aaaatatccc aagcttgaat ctgaatttgt ttacggagac
     5221 tataaagtgt acgatgttag gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc
     5281 accgctaagt acttctttta cagcaatatt atgaattttt tcaagaccga gattacactg
     5341 gccaatggag agattcggaa gcgaccactt atcgaaacaa acggagaaac aggagaaatc
     5401 gtgtgggaca agggtaggga tttcgcgaca gtccggaagg tcctgtccat gccgcaggtg
     5461 aacatcgtta aaaagaccga agtacagacc ggaggcttct ccaaggaaag tatcctcccg
     5521 aaaaggaaca gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc
     5581 ggattcgttt ctcctacagt cgcttacagt gtactggttg tggccaaagt ggagaaaggg
     5641 aagtctaaaa aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca
     5701 agcttcgaaa aaaaccccat cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa
     5761 gacctcatca ttaagcttcc caagtactct ctctttgagc ttgaaaacgg ccggaaacga
     5821 atgctcgcta gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac
     5881 gttaatttct tgtatctggc cagccactat gaaaagctca aagggtctcc cgaagataat
     5941 gagcagaagc agctgttcgt ggaacaacac aaacactacc ttgatgagat catcgagcaa
     6001 ataagcgaat tctccaaaag agtgatcctc gccgacgcta acctcgataa ggtgctttct
     6061 gcttacaata agcacaggga taagcccatc agggagcagg cagaaaacat tatccacttg
     6121 tttactctga ccaacttggg cgcgcctgca gccttcaagt acttcgacac caccatagac
     6181 agaaagcaat acagatctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt
     6241 acggggctct atgaaacaag aatcgacctc tctcagctcg gtggagactc tggtggttca
     6301 actaatttat ctgatattat tgaaaaagaa acaggtaaac aattagttat tcaagaatca
     6361 attttaatgt tgccagaaga agttgaagaa gttattggta ataaaccaga atctgatatt
     6421 ttagttcata ctgcttatga tgaatctact gatgaaaatg ttatgttatt gacttcagat
     6481 gctccagaat ataaaccatg ggctttagtt attcaagatt ctaatggtga aaataaaatt
     6541 aaaatgttgt ctggtggttc acccaagaag aagaggaagg tgtgatctct tctcgagtca
     6601 tgtaattagt tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa
     6661 aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata gttatgttag
     6721 tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg
     6781 catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa
     6841 tttgcggccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     6901 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6961 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     7021 acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg
     7081 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7141 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7201 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7261 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7321 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7381 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7441 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     7501 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatcga
     7561 ccggtcgagg agaacttcta gtatatctac atacctaata ttattgcctt attaaaaatg
     7621 gaatcccaac aattacatca aaatccacat tctcttcaaa atcaattgtc ctgtacttcc
     7681 ttgttcatgt gtgttcaaaa acgttatatt tataggataa ttatactcta tttctcaaca
     7741 agtaattggt tgtttggccg agcggtctaa ggcgcctgat tcaagaaata tcttgaccgc
     7801 agttaactgt gggaatactc aggtatcgta agatgcaaga gttcgaatct cttagcaacc
     7861 attatttttt tcctcaacat aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt
     7921 cgatgactgg aaattttttg ttaatttcag aggtcgcctg acgcatatac ctttttcaac
     7981 tgaaaaattg ggagaaaaag gaaaggtgag agcgccggaa ccggcttttc atatagaata
     8041 gagaagcgtt catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa
     8101 ggacctattg ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac
     8161 cttttacatt tcagcaatat atatatatat atttcaagga tataccattc taatgtctgc
     8221 ccctaagaag atcgtcgttt tgccaggtga ccacgttggt caagaaatca cagccgaagc
     8281 cattaaggtt cttaaagcta tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa
     8341 tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtt ccacttccag atgaggcgct
     8401 ggaagcctcc aagaaggctg atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg
     8461 taccggtagt gttagacctg aacaaggttt actaaaaatc cgtaaagaac ttcaattgta
     8521 cgccaactta agaccatgta actttgcatc cgactctctt ttagacttat ctccaatcaa
     8581 gccacaattt gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg gaggtattta
     8641 ctttggtaag agaaaggaag acgatggtga tggtgtcgct tgggatagtg aacaatacac
     8701 cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc atggccctac aacatgagcc
     8761 accattgcct atttggtcct tggataaagc taatgttttg gcctcttcaa gattatggag
     8821 aaaaactgtg gaggaaacca tcaagaacga attccctaca ttgaaggttc aacatcaatt
     8881 gattgattct gccgccatga tcctagttaa gaacccaacc cacctaaatg gtattataat
     8941 caccagcaac atgtttggtg atatcatctc cgatgaagcc tccgttatcc caggttcctt
     9001 gggtttgttg ccatctgcgt ccttggcctc tttgccagac aagaacaccg catttggttt
     9061 gtacgaacca tgccacggtt ctgctccaga tttgccaaag aataaggtca accctatcgc
     9121 cactatcttg tctgctgcaa tgatgttgaa attgtcattg aacttgcctg aagaaggtaa
     9181 ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt atcagaactg gtgatttagg
     9241 tggttccaac agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct
     9301 tgcttaaaaa gattctcttt ttttatgata tttgtacata aactttataa atgaaattca
     9361 taatagaaac gacacgaaat tacaaaatgg aatatgttca tagggtagac gaaactatat
     9421 acgcaatcta catacattta tcaagaagga gaaaaaggag gatgtaaagg aatacaggta
     9481 agcaaattga tactaatggc tcaacgtgat aaggaaaaag aattgcactt taacattaat
     9541 attgacaagg aggagggcac cacacaaaaa gttaggtgta acagaaaatc atgaaactat
     9601 gattcctaat ttatatattg gaggattttc tctaaaaaaa aaaaaataca acaaataaaa
     9661 aacactcaat gacctgacca tttgatggag tttaagtcaa taccttcttg aaccatttcc
     9721 cataatggtg aaagttccct caagaatttt actctgtcag aaacggcctt aacgacgtag
     9781 tcgatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     9841 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     9901 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     9961 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
    10021 ataatggttt cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa
    10081 tgatggaata atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat
    10141 tttagaaagt aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa
    10201 ggtttaaaaa atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc
    10261 agattaaata gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg
    10321 tgtgtggtct tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa
    10381 gagcaagata aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa
    10441 aacaaaaact attttttctt taatttcttt ttttactttc tatttttaat ttatatattt
    10501 atattaaaaa atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg
    10561 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    10621 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    10681 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    10741 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    10801 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    10861 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    10921 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    10981 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    11041 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    11101 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    11161 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    11221 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    11281 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    11341 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    11401 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    11461 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    11521 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    11581 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    11641 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    11701 gatcaaagga tcttc
//
