LOCUS       sequence-305898-       11456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding TRV2 with direct repeat multiplexed truncated FT 
            augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11456)
  AUTHORS   Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
            DF
  TITLE     Multiplexed heritable gene editing using RNA viruses and mobile 
            single guide RNAs.
  JOURNAL   Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y.
  PUBMED    32483329
REFERENCE   2  (bases 1 to 11456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y."
COMMENT     Sequence Label: pEE495
FEATURES             Location/Qualifiers
     source          1..11456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        427..772
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     715..735
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_RNA        2327..2402
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        2533..2608
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        2739..2814
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             3386..3403
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3438..3690
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3712..3736
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5037..5666
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6095..7168
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7234..7428
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7664..7686)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7772..7912
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7827..7846
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7927..7944)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8098..8686)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8178..8197)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10111..10905)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11330..11354
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
      781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
      841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
      901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
      961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
     1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
     1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
     1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
     1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
     1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
     1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
     1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
     1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
     1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
     1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
     1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
     1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
     1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
     1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
     1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
     1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
     1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
     2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
     2101 ttgagtttcg gagcatcttg ttctggggtt tcacactatc tttagagaaa gtgttaagtt
     2161 aattaagtta tcttaattaa gagcataatt atactgattt gtctctcgtt gatagagtct
     2221 atcattctgt tactaaaaat ttgacaactc ggtttgctga cctactggtt actgtatcac
     2281 ttacccgagt taacgagtct agaaacgtgt gaaagaaaca attgaggttt tagagctaga
     2341 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     2401 gctccctagt ctataaatat aagagaccct cttatagtaa gcagagttgt tggagacgtt
     2461 cttgatccgt ttaatagatc aatcactcta aaggttactt atggccaagt gcttggtagt
     2521 agcgactcca tggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     2581 acttgaaaaa gtggcaccga gtcggtgctc cctagtctat aaatataaga gaccctctta
     2641 tagtaagcag agttgttgga gacgttcttg atccgtttaa tagatcaatc actctaaagg
     2701 ttacttatgg ccaacaggtt gattgtcttc tcaagcaggt tttagagcta gaaatagcaa
     2761 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctcccta
     2821 gtctataaat ataagagacc ctcttatagt aagcagagtt gttggagacg ttcttgatcc
     2881 gtttaataga tcaatcactc taaaggttac ttatggccaa agtgccgggc atgtcccgaa
     2941 gacattaaac tacggttctt taagtagatc cgtgtctgaa gttttaggtt caatttaaac
     3001 ctacgagatt gacattctcg actgatcttg attgatcggt aagtcttttg taatttaatt
     3061 ttctttttga ttttatttta aattgttatc tgtttctgtg tatagactgt ttgagatcgg
     3121 cgtttggccg actcattgtc ttaccatagg ggaacggact ttgtttgtgt tgttatttta
     3181 tttgtatttt attaaaattc tcaacgatct gaaaaagcct cgcggctaag agattgttgg
     3241 ggggtgagta agtactttta aagtgatgat ggttacaaag gcaaaagggg taaaacccct
     3301 cgcctacgta agcgttatta cgcccgtctg tacttatatc agtacactga cgagtcccta
     3361 aaggacgaaa cgggagaacg ctagccacca ccaccaccac cacgtgtgaa ttacaggtga
     3421 ccagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     3481 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     3541 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     3601 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     3661 cgcgcgcggt gtcatctatg ttactagatc gggaattaaa ctatcagtgt ttgacaggat
     3721 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag
     3781 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc
     3841 tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg
     3901 ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc
     3961 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa
     4021 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct
     4081 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca
     4141 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc
     4201 ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct
     4261 agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc
     4321 cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg
     4381 catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg
     4441 cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac
     4501 cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt
     4561 gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg
     4621 cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt
     4681 gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa
     4741 ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg
     4801 atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa
     4861 atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa
     4921 gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat
     4981 gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga
     5041 aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc
     5101 tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg
     5161 gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg
     5221 accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg
     5281 acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc
     5341 tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg
     5401 ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg
     5461 cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc
     5521 ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca
     5581 caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg
     5641 ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac
     5701 aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag
     5761 cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac
     5821 caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata
     5881 catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg
     5941 ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca
     6001 tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca
     6061 atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc
     6121 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc
     6181 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc
     6241 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa
     6301 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac
     6361 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg
     6421 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc
     6481 ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac
     6541 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc
     6601 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga
     6661 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa
     6721 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa
     6781 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat
     6841 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt
     6901 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa
     6961 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt
     7021 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga
     7081 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat
     7141 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct
     7201 agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc
     7261 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa
     7321 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa
     7381 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca
     7441 gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc
     7501 cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc
     7561 aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca
     7621 tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     7681 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     7741 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     7801 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     7861 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     7921 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7981 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8041 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8101 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8161 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8221 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8281 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8341 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8401 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8461 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8521 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8581 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8641 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8701 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaagggc tgatgaatcc
     8761 cctaatgatt tttatcaaaa tcattaagtt aaggtagata cacatcttgt catatgatca
     8821 aatggtttcg ccaaaaatca ataatcagac aacaaaatgt gcgaactcga tattttacac
     8881 gactctcttt accaattctg ccccgaatta cacttaaaac gactcaacag cttaacgttg
     8941 gcttgccacg ccttacttga ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg
     9001 ccaaccaaag cgagaacaaa acataacatc aaacgaatcg accgattgtt aggtaatcgt
     9061 cacctccaca aagagcgact cgctgtatac cgttggcatg ctagctttat ctgttcgggc
     9121 aatacgatgc ccattgtact tgttgactgg tctgatatcc gtgagcaaaa acggcttatg
     9181 gtattgcgag cttcagtcgc actacacggt cgttctgtta ctctttatga gaaagcgttc
     9241 ccgctttcag agcaatgttc aaagaaagct catgaccaat ttctagccga ccttgcgagc
     9301 attctaccga gtaacaccac accgctcatt gtcagtgatg ctggctttaa agtgccatgg
     9361 tataaatccg ttgagaagct gggttggtac tggttaagtc gagtaagagg aaaagtacaa
     9421 tatgcagacc taggagcgga aaactggaaa cctatcagca acttacatga tatgtcatct
     9481 agtcactcaa agactttagg ctataagagg ctgactaaaa gcaatccaat ctcatgccaa
     9541 attctattgt ataaatctcg ctctaaaggc cgaaaaaatc agcgctcgac acggactcat
     9601 tgtcaccacc cgtcacctaa aatctactca gcgtcggcaa aggagccatg gattctagca
     9661 actaacttac ctgttgaaat tcgaacaccc aaacaacttg ttaatatcta ttcgaagcga
     9721 atgcagattg aagaaacctt ccgagacttg aaaagtcctg cctacggact aggcctacgc
     9781 catagccgaa cgagcagctc agagcgtttt gatatcatgc tgctaatcgc cctgatgctt
     9841 caactaacat gttggcttgc gggcgttcat gctcagaaac aaggttggga caagcacttc
     9901 caggctaaca cagtcagaaa tcgaaacgta ctctcaacag ttcgcttagg catggaagtt
     9961 ttgcggcatt ctggctacac aataacaagg gaagactcac tcgtggctgc aaccctgctt
    10021 actcaaaatc tattcacaca tggttacgtt ttggggaaat tatgagggga tctctcagcg
    10081 ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta aaatataata
    10141 ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg
    10201 ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc
    10261 cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat
    10321 gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt
    10381 taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca
    10441 atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa
    10501 gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc
    10561 atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac
    10621 gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac
    10681 ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc
    10741 ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc
    10801 caccagctta tataccttag caggagacat tccttccgta tcttttacgc agcggtattt
    10861 ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc
    10921 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
    10981 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
    11041 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag
    11101 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
    11161 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
    11221 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
    11281 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
    11341 attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa
    11401 tgtactgaat taacgccgaa ttaattccta ggccaccatg ttgggcccgg cgcgcc
//
