LOCUS       sequence-305899-       11824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding TRV2 complete genome with MCS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11824)
  AUTHORS   Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
            DF
  TITLE     Multiplexed heritable gene editing using RNA viruses and mobile 
            single guide RNAs.
  JOURNAL   Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y.
  PUBMED    32483329
REFERENCE   2  (bases 1 to 11824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y."
COMMENT     Sequence Label: pEE515
FEATURES             Location/Qualifiers
     source          1..11824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        427..772
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     715..735
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_RNA        2324..2399
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             3263..3568
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3447..3466
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             4045..4062
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4097..4349
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4371..4395
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5696..6325
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6754..7827
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7893..8087
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8323..8345)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8431..8571
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8486..8505
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8586..8603)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8757..9345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8837..8856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10479..11273)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11698..11722
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
      781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
      841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
      901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
      961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
     1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
     1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
     1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
     1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
     1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
     1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
     1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
     1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
     1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
     1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
     1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
     1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
     1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
     1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
     1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
     1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
     1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
     2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
     2101 ttgagtttcg gagcatcttg ttctggggtt tcacactatc tttagagaaa gtgttaagtt
     2161 aattaagtta tcttaattaa gagcataatt atactgattt gtctctcgtt gatagagtct
     2221 atcattctgt tactaaaaat ttgacaactc ggtttgctga cctactggtt actgtatcac
     2281 ttacccgagt taacgagtct agaaacggcg gcaggtgcca tgggttttag agctagaaat
     2341 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     2401 ccctagtcta taaatataag agaccctctt atagtaagca gagttgttgg agacgttctt
     2461 gatccgttta atagatcaat cactctaaag gttacttatg gccaaagaga ggtgactaat
     2521 ggcttggatc taaggccttc tcaggttcaa aacaagccaa gagttgagat tggtggagaa
     2581 gacctcagga acttctatac tttggttatg gtggatccag atgttccaag tcctagcaac
     2641 cctcacctcc gagaatatct ccattggttg gtgactgata tccctgctac aactggaaca
     2701 acctttggca atgagattgt gtgttacgaa aatccaagtc ccactgcagg aattcatcgt
     2761 gtcgtgttta tattgtttcg acagcttggc aggcaaacag tgtatgcacc agggtggcgc
     2821 cagaacttca acactcgcga gtttgctgag atctacaatc tcggccttcc cgtggccgca
     2881 gttttctaca attgtcagag ggagagtggc tgcggaggaa gaagacttta gagtggttta
     2941 aacaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     3001 ctgatatgta tacccgaagt atgtcaaaag gaggtatgct atgaagcagc gtattacagt
     3061 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     3121 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     3181 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     3241 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     3301 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     3361 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     3421 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     3481 ttccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     3541 cattaacctg atgttctggg gaatataaca cctgcgctca gtgccgggca tgtcccgaag
     3601 acattaaact acggttcttt aagtagatcc gtgtctgaag ttttaggttc aatttaaacc
     3661 tacgagattg acattctcga ctgatcttga ttgatcggta agtcttttgt aatttaattt
     3721 tctttttgat tttattttaa attgttatct gtttctgtgt atagactgtt tgagatcggc
     3781 gtttggccga ctcattgtct taccataggg gaacggactt tgtttgtgtt gttattttat
     3841 ttgtatttta ttaaaattct caacgatctg aaaaagcctc gcggctaaga gattgttggg
     3901 gggtgagtaa gtacttttaa agtgatgatg gttacaaagg caaaaggggt aaaacccctc
     3961 gcctacgtaa gcgttattac gcccgtctgt acttatatca gtacactgac gagtccctaa
     4021 aggacgaaac gggagaacgc tagccaccac caccaccacc acgtgtgaat tacaggtgac
     4081 cagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
     4141 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
     4201 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     4261 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     4321 gcgcgcggtg tcatctatgt tactagatcg ggaattaaac tatcagtgtt tgacaggata
     4381 tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg
     4441 gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct
     4501 cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt
     4561 tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct
     4621 gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat
     4681 acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg
     4741 ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac
     4801 gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg
     4861 gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta
     4921 gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc
     4981 ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc
     5041 atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc
     5101 acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc
     5161 ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg
     5221 aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc
     5281 agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg
     5341 accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac
     5401 cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga
     5461 tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa
     5521 tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag
     5581 aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg
     5641 cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa
     5701 ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct
     5761 agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg
     5821 cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga
     5881 ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga
     5941 cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct
     6001 gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt
     6061 taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc
     6121 gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc
     6181 cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac
     6241 aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc
     6301 tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca
     6361 aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc
     6421 ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc
     6481 aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac
     6541 atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc
     6601 taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat
     6661 gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa
     6721 tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg
     6781 gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc
     6841 gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct
     6901 gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag
     6961 ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc
     7021 cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga
     7081 gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg
     7141 gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc
     7201 gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca
     7261 cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac
     7321 ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag
     7381 gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag
     7441 atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg
     7501 taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt
     7561 ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag
     7621 gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc
     7681 aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat
     7741 ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc
     7801 gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta
     7861 gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca
     7921 aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac
     7981 cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac
     8041 tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag
     8101 cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc
     8161 gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca
     8221 ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat
     8281 caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     8341 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     8401 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     8461 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     8521 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     8581 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8641 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8701 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8761 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8821 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8881 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8941 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9001 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9061 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9121 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9181 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9241 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9301 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9361 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggct gatgaatccc
     9421 ctaatgattt ttatcaaaat cattaagtta aggtagatac acatcttgtc atatgatcaa
     9481 atggtttcgc caaaaatcaa taatcagaca acaaaatgtg cgaactcgat attttacacg
     9541 actctcttta ccaattctgc cccgaattac acttaaaacg actcaacagc ttaacgttgg
     9601 cttgccacgc cttacttgac tgtaaaactc tcactcttac cgaacttggc cgtaacctgc
     9661 caaccaaagc gagaacaaaa cataacatca aacgaatcga ccgattgtta ggtaatcgtc
     9721 acctccacaa agagcgactc gctgtatacc gttggcatgc tagctttatc tgttcgggca
     9781 atacgatgcc cattgtactt gttgactggt ctgatatccg tgagcaaaaa cggcttatgg
     9841 tattgcgagc ttcagtcgca ctacacggtc gttctgttac tctttatgag aaagcgttcc
     9901 cgctttcaga gcaatgttca aagaaagctc atgaccaatt tctagccgac cttgcgagca
     9961 ttctaccgag taacaccaca ccgctcattg tcagtgatgc tggctttaaa gtgccatgga
    10021 ttctagcaac taacttacct gttgaaattc gaacacccaa acaacttgtt aatatctatt
    10081 cgaagcgaat gcagattgaa gaaaccttcc gagacttgaa aagtcctgcc tacggactag
    10141 gcctacgcca tagccgaacg agcagctcag agcgttttga tatcatgctg ctaatcgccc
    10201 tgatgcttca actaacatgt tggcttgcgg gcgttcatgc tcagaaacaa ggttgggaca
    10261 agcacttcca ggctaacaca gtcagaaatc gaaacgtact ctcaacagtt cgcttaggca
    10321 tggaagtttt gcggcattct ggctacacaa taacaaggga agactcactc gtggctgcaa
    10381 ccctgcttac tcaaaatcta ttcacacatg gttacgtttt ggggaaatta tgaggggatc
    10441 tctcagcgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa
    10501 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
    10561 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
    10621 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
    10681 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
    10741 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
    10801 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
    10861 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
    10921 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
    10981 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
    11041 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
    11101 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
    11161 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
    11221 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
    11281 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
    11341 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
    11401 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt
    11461 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
    11521 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
    11581 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
    11641 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
    11701 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac
    11761 gtttttaatg tactgaatta acgccgaatt aattcctagg ccaccatgtt gggcccggcg
    11821 cgcc
//
