LOCUS       sequence-305931-       11589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing base editor A3A-Delta-182-BE3 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11589)
  AUTHORS   Tan J, Zhang F, Karcher D, Bock R
  TITLE     Expanding the genome-targeting scope and the site selectivity of 
            high-precision base editors.
  JOURNAL   Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.
  PUBMED    32005820
REFERENCE   2  (bases 1 to 11589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-31"; volume: "11(1):629. doi"; pages: " 
            10.1038/s41467-020-14465-z"
COMMENT     Sequence Label: pJT170_GalL_A3A-Delta-182-BE3
FEATURES             Location/Qualifiers
     source          1..11589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1439..1462
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     1483..1499
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1483..1499
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(2059..2085,2086..2088,2089..6162)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2059..6162
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6175..6423
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6436..6456
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6472..6719
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6488..6506)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(6737..6756)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6738..6756)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6766..6783)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6766..6782)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6775..6797)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6923..7378
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7010..7029)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7220..7241
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7679..8086
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             8087..9181
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(9675..9694)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9794..9816
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9854..9872)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    9914..10417
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        10454..10558
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10559..10627,10628..11419)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10777..10796)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcgt gacagccctc cgaaggaaga ctctcctccg
     1081 tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga tgtgcctcgc gccgcactgc
     1141 tccgaacaat aaagattcta caatactagc ttttatggtt atgaagagga aaaattggca
     1201 gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat taacaaccat aggatgataa
     1261 tgcgattagt tttttagcct tatttctggg gtaattaatc agcgaagcga tgatttttga
     1321 tctattaacg gatatataaa tgcaaaaact gcataaccac tttaactaat actttcaaca
     1381 ttttcggttt gtattacttc ttattcaaat gtaataaaag tatcaacaaa aaattgttaa
     1441 tatacctcta tactttaacg tcaaggagaa aaaaccccgg attctagaac tagtggatcc
     1501 cccgggaaaa aaatggaagc cagcccagca tccgggccca gacacttgat ggatccacac
     1561 atattcactt ccaactttaa caatggcatt ggaaggcata agacctacct gtgctacgaa
     1621 gtggagcgcc tggacaatgg cacctcggtc aagatggacc agcacagggg ctttctacac
     1681 aaccaggcta agaatcttct ctgtggcttt tacggccgcc atgcggagct gcgcttcttg
     1741 gacctggttc cttctttgca gttggacccg gcccagatct acagggtcac ttggttcatc
     1801 tcctggagcc cctgcttctc ctggggctgt gccggggaag tgcgtgcgtt ccttcaggag
     1861 aacacacacg tgagactgcg tatcttcgct gcccgcatct atgattacga ccccctatat
     1921 aaggaggcac tgcaaatgct gcgggatgct ggggcccaag tctccatcat gacctacgat
     1981 gaatttaagc actgctggga cacctttgtg gaccaccagg gatgtccctt ccagccctgg
     2041 gatggactag atgagcacat ggacaagaag tactccattg ggctcgctat cggcacaaac
     2101 agcgtcggtt gggccgtcat tacggacgag tacaaggtgc cgagcaaaaa attcaaagtt
     2161 ctgggcaata ccgatcgcca cagcataaag aagaacctca ttggcgccct cctgttcgac
     2221 tccggggaga cggccgaagc cacgcggctc aaaagaacag cacggcgcag atatacccgc
     2281 agaaagaatc ggatctgcta cctgcaggag atctttagta atgagatggc taaggtggat
     2341 gactctttct tccataggct ggaggagtcc tttttggtgg aggaggataa aaagcacgag
     2401 cgccacccaa tctttggcaa tatcgtggac gaggtggcgt accatgaaaa gtacccaacc
     2461 atatatcatc tgaggaagaa gcttgtagac agtactgata aggctgactt gcggttgatc
     2521 tatctcgcgc tggcgcatat gatcaaattt cggggacact tcctcatcga gggggacctg
     2581 aacccagaca acagcgatgt cgacaaactc tttatccaac tggttcagac ttacaatcag
     2641 cttttcgaag agaacccgat caacgcatcc ggagttgacg ccaaagcaat cctgagcgct
     2701 aggctgtcca aatcccggcg gctcgaaaac ctcatcgcac agctccctgg ggagaagaag
     2761 aacggcctgt ttggtaatct tatcgccctg tcactcgggc tgacccccaa ctttaaatct
     2821 aacttcgacc tggccgaaga tgccaagctt caactgagca aagacaccta cgatgatgat
     2881 ctcgacaatc tgctggccca gatcggcgac cagtacgcag accttttttt ggcggcaaag
     2941 aacctgtcag acgccattct gctgagtgat attctgcgag tgaacacgga gatcaccaaa
     3001 gctccgctga gcgctagtat gatcaagcgc tatgatgagc accaccaaga cttgactttg
     3061 ctgaaggccc ttgtcagaca gcaactgcct gagaagtaca aggaaatttt cttcgatcag
     3121 tctaaaaatg gctacgccgg atacattgac ggcggagcaa gccaggagga attttacaaa
     3181 tttattaagc ccatcttgga aaaaatggac ggcaccgagg agctgctggt aaagcttaac
     3241 agagaagatc tgttgcgcaa acagcgcact ttcgacaatg gaagcatccc ccaccagatt
     3301 cacctgggcg aactgcacgc tatcctcagg cggcaagagg atttctaccc ctttttgaaa
     3361 gataacaggg aaaagattga gaaaatcctc acatttcgga taccctacta tgtaggcccc
     3421 ctcgcccggg gaaattccag attcgcgtgg atgactcgca aatcagaaga gaccatcact
     3481 ccctggaact tcgaggaagt cgtggataag ggggcctctg cccagtcctt catcgaaagg
     3541 atgactaact ttgataaaaa tctgcctaac gaaaaggtgc ttcctaaaca ctctctgctg
     3601 tacgagtact tcacagttta taacgagctc accaaggtca aatacgtcac agaagggatg
     3661 agaaagccag cattcctgtc tggagagcag aagaaagcta tcgtggacct cctcttcaag
     3721 acgaaccgga aagttaccgt gaaacagctc aaagaagact atttcaaaaa gattgaatgt
     3781 ttcgactctg ttgaaatcag cggagtggag gatcgcttca acgcatccct gggaacgtat
     3841 cacgatctcc tgaaaatcat taaagacaag gacttcctgg acaatgagga gaacgaggac
     3901 attcttgagg acattgtcct cacccttacg ttgtttgaag atagggagat gattgaagaa
     3961 cgcttgaaaa cttacgctca tctcttcgac gacaaagtca tgaaacagct caagaggcgc
     4021 cgatatacag gatgggggcg gctgtcaaga aaactgatca atgggatccg agacaagcag
     4081 agtggaaaga caatcctgga ttttcttaag tccgatggat ttgccaaccg gaacttcatg
     4141 cagttgatcc atgatgactc tctcaccttt aaggaggaca tccagaaagc acaagtttct
     4201 ggccaggggg acagtcttca cgagcacatc gctaatcttg caggtagccc agctatcaaa
     4261 aagggaatac tgcagaccgt taaggtcgtg gatgaactcg tcaaagtaat gggaaggcat
     4321 aagcccgaga atatcgttat cgagatggcc cgagagaacc aaactaccca gaagggacag
     4381 aagaacagta gggaaaggat gaagaggatt gaagagggta taaaagaact ggggtcccaa
     4441 atccttaagg aacacccagt tgaaaacacc cagcttcaga atgagaagct ctacctgtac
     4501 tacctgcaga acggcaggga catgtacgtg gatcaggaac tggacatcaa tcggctctcc
     4561 gactacgacg tggatcatat cgtgccccag tcttttctca aagatgattc tattgataat
     4621 aaagtgttga caagatccga taaaaataga gggaagagtg ataacgtccc ctcagaagaa
     4681 gttgtcaaga aaatgaaaaa ttattggcgg cagctgctga acgccaaact gatcacacaa
     4741 cggaagttcg ataatctgac taaggctgaa cgaggtggcc tgtctgagtt ggataaagcc
     4801 ggcttcatca aaaggcagct tgttgagaca cgccagatca ccaagcacgt ggcccaaatt
     4861 ctcgattcac gcatgaacac caagtacgat gaaaatgaca aactgattcg agaggtgaaa
     4921 gttattactc tgaagtctaa gctggtctca gatttcagaa aggactttca gttttataag
     4981 gtgagagaga tcaacaatta ccaccatgcg catgatgcct acctgaatgc agtggtaggc
     5041 actgcactta tcaaaaaata tcccaagctt gaatctgaat ttgtttacgg agactataaa
     5101 gtgtacgatg ttaggaaaat gatcgcaaag tctgagcagg aaataggcaa ggccaccgct
     5161 aagtacttct tttacagcaa tattatgaat tttttcaaga ccgagattac actggccaat
     5221 ggagagattc ggaagcgacc acttatcgaa acaaacggag aaacaggaga aatcgtgtgg
     5281 gacaagggta gggatttcgc gacagtccgg aaggtcctgt ccatgccgca ggtgaacatc
     5341 gttaaaaaga ccgaagtaca gaccggaggc ttctccaagg aaagtatcct cccgaaaagg
     5401 aacagcgaca agctgatcgc acgcaaaaaa gattgggacc ccaagaaata cggcggattc
     5461 gattctccta cagtcgctta cagtgtactg gttgtggcca aagtggagaa agggaagtct
     5521 aaaaaactca aaagcgtcaa ggaactgctg ggcatcacaa tcatggagcg atcaagcttc
     5581 gaaaaaaacc ccatcgactt tctcgaggcg aaaggatata aagaggtcaa aaaagacctc
     5641 atcattaagc ttcccaagta ctctctcttt gagcttgaaa acggccggaa acgaatgctc
     5701 gctagtgcgg gcgagctgca gaaaggtaac gagctggcac tgccctctaa atacgttaat
     5761 ttcttgtatc tggccagcca ctatgaaaag ctcaaagggt ctcccgaaga taatgagcag
     5821 aagcagctgt tcgtggaaca acacaaacac taccttgatg agatcatcga gcaaataagc
     5881 gaattctcca aaagagtgat cctcgccgac gctaacctcg ataaggtgct ttctgcttac
     5941 aataagcaca gggataagcc catcagggag caggcagaaa acattatcca cttgtttact
     6001 ctgaccaact tgggcgcgcc tgcagccttc aagtacttcg acaccaccat agacagaaag
     6061 cggtacacct ctacaaagga ggtcctggac gccacactga ttcatcagtc aattacgggg
     6121 ctctatgaaa caagaatcga cctctctcag ctcggtggag actctggtgg ttcaactaat
     6181 ttatctgata ttattgaaaa agaaacaggt aaacaattag ttattcaaga atcaatttta
     6241 atgttgccag aagaagttga agaagttatt ggtaataaac cagaatctga tattttagtt
     6301 catactgctt atgatgaatc tactgatgaa aatgttatgt tattgacttc agatgctcca
     6361 gaatataaac catgggcttt agttattcaa gattctaatg gtgaaaataa aattaaaatg
     6421 ttgtctggtg gttcacccaa gaagaagagg aaggtgtgat ctcttctcga gtcatgtaat
     6481 tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag
     6541 gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa
     6601 gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta
     6661 acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg
     6721 gccggtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     6781 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     6841 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     6901 gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6961 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     7021 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     7081 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     7141 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     7201 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     7261 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     7321 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc
     7381 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tcgaccggtc
     7441 gaggagaact tctagtatat ctacatacct aatattattg ccttattaaa aatggaatcc
     7501 caacaattac atcaaaatcc acattctctt caaaatcaat tgtcctgtac ttccttgttc
     7561 atgtgtgttc aaaaacgtta tatttatagg ataattatac tctatttctc aacaagtaat
     7621 tggttgtttg gccgagcggt ctaaggcgcc tgattcaaga aatatcttga ccgcagttaa
     7681 ctgtgggaat actcaggtat cgtaagatgc aagagttcga atctcttagc aaccattatt
     7741 tttttcctca acataacgag aacacacagg ggcgctatcg cacagaatca aattcgatga
     7801 ctggaaattt tttgttaatt tcagaggtcg cctgacgcat ataccttttt caactgaaaa
     7861 attgggagaa aaaggaaagg tgagagcgcc ggaaccggct tttcatatag aatagagaag
     7921 cgttcatgac taaatgcttg catcacaata cttgaagttg acaatattat ttaaggacct
     7981 attgtttttt ccaataggtg gttagcaatc gtcttacttt ctaacttttc ttacctttta
     8041 catttcagca atatatatat atatatttca aggatatacc attctaatgt ctgcccctaa
     8101 gaagatcgtc gttttgccag gtgaccacgt tggtcaagaa atcacagccg aagccattaa
     8161 ggttcttaaa gctatttctg atgttcgttc caatgtcaag ttcgatttcg aaaatcattt
     8221 aattggtggt gctgctatcg atgctacagg tgttccactt ccagatgagg cgctggaagc
     8281 ctccaagaag gctgatgccg ttttgttagg tgctgtgggt ggtcctaaat ggggtaccgg
     8341 tagtgttaga cctgaacaag gtttactaaa aatccgtaaa gaacttcaat tgtacgccaa
     8401 cttaagacca tgtaactttg catccgactc tcttttagac ttatctccaa tcaagccaca
     8461 atttgctaaa ggtactgact tcgttgttgt cagagaatta gtgggaggta tttactttgg
     8521 taagagaaag gaagacgatg gtgatggtgt cgcttgggat agtgaacaat acaccgttcc
     8581 agaagtgcaa agaatcacaa gaatggccgc tttcatggcc ctacaacatg agccaccatt
     8641 gcctatttgg tccttggata aagctaatgt tttggcctct tcaagattat ggagaaaaac
     8701 tgtggaggaa accatcaaga acgaattccc tacattgaag gttcaacatc aattgattga
     8761 ttctgccgcc atgatcctag ttaagaaccc aacccaccta aatggtatta taatcaccag
     8821 caacatgttt ggtgatatca tctccgatga agcctccgtt atcccaggtt ccttgggttt
     8881 gttgccatct gcgtccttgg cctctttgcc agacaagaac accgcatttg gtttgtacga
     8941 accatgccac ggttctgctc cagatttgcc aaagaataag gtcaacccta tcgccactat
     9001 cttgtctgct gcaatgatgt tgaaattgtc attgaacttg cctgaagaag gtaaggccat
     9061 tgaagatgca gttaaaaagg ttttggatgc aggtatcaga actggtgatt taggtggttc
     9121 caacagtacc accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta
     9181 aaaagattct ctttttttat gatatttgta cataaacttt ataaatgaaa ttcataatag
     9241 aaacgacacg aaattacaaa atggaatatg ttcatagggt agacgaaact atatacgcaa
     9301 tctacataca tttatcaaga aggagaaaaa ggaggatgta aaggaataca ggtaagcaaa
     9361 ttgatactaa tggctcaacg tgataaggaa aaagaattgc actttaacat taatattgac
     9421 aaggaggagg gcaccacaca aaaagttagg tgtaacagaa aatcatgaaa ctatgattcc
     9481 taatttatat attggaggat tttctctaaa aaaaaaaaaa tacaacaaat aaaaaacact
     9541 caatgacctg accatttgat ggagtttaag tcaatacctt cttgaaccat ttcccataat
     9601 ggtgaaagtt ccctcaagaa ttttactctg tcagaaacgg ccttaacgac gtagtcgata
     9661 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     9721 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     9781 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     9841 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     9901 gtttcttagg acggatcgct tgcctgtaac ttacacgcgc ctcgtatctt ttaatgatgg
     9961 aataatttgg gaatttactc tgtgtttatt tatttttatg ttttgtattt ggattttaga
    10021 aagtaaataa agaaggtaga agagttacgg aatgaagaaa aaaaaataaa caaaggttta
    10081 aaaaatttca acaaaaagcg tactttacat atatatttat tagacaagaa aagcagatta
    10141 aatagatata cattcgatta acgataagta aaatgtaaaa tcacaggatt ttcgtgtgtg
    10201 gtcttctaca cagacaagat gaaacaattc ggcattaata cctgagagca ggaagagcaa
    10261 gataaaaggt agtatttgtt ggcgatcccc ctagagtctt ttacatcttc ggaaaacaaa
    10321 aactattttt tctttaattt ctttttttac tttctatttt taatttatat atttatatta
    10381 aaaaatttaa attataatta tttttatagc acgtgatgaa aaggacccag gtggcacttt
    10441 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
    10501 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    10561 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    10621 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
    10681 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
    10741 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    10801 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    10861 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    10921 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    10981 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    11041 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    11101 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    11161 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    11221 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    11281 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    11341 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    11401 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    11461 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    11521 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    11581 aggatcttc
//
