LOCUS       sequence-305932-        8961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression vector to generate a hypoxia fate-mapping 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Godet I, Shin YJ, Ju JA, Ye IC, Wang G, Gilkes DM
  TITLE     Fate-mapping post-hypoxic tumor cells reveals a ROS-resistant 
            phenotype that promotes metastasis.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4862. doi: 10.1038/s41467-019-12412-1.
  PUBMED    31649238
REFERENCE   2  (bases 1 to 8961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4862. doi"; pages: " 
            10.1038/s41467-019-12412-1"
COMMENT     Sequence Label: 4xHRE-MinTK-CRE-ODD
FEATURES             Location/Qualifiers
     source          1..8961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1797..1914
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2064..2095
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     intron          2169..2741
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     2749..2768
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        2796..2814
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2830..2848
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2830..2847
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             2883..3911
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     LTR             5511..5744
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5816..5937
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5853..5872)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5907..5926
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(6102..6120)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6130..6147)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6130..6146)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6139..6161)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6288..6743
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6375..6394)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6585..6606
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6769..6873
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6874..6942,6943..7734)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7092..7111)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7905..8493
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8394..8413
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8647..8664
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8781..8802
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8817..8822,8823..8840,8841..8847)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8855..8871
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8860..8882
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8879..8895
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8879..8895
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8914..8934
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8916..8934
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggtttt
     1801 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     1861 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttatcgac
     1921 tcagaacacg ccgtgtacgt gcttcacgtg tacgtggcga tcgtgtacgt gctgtctcac
     1981 acagcactct agactactcg tgtacgtgta cacgaagctt cgtagcaatc ggaccgacga
     2041 cgccgggctc gagatctaga ctctagaggg tatataatgg aagctcgaat tccagcttgg
     2101 cattccggta ctgttggtaa aaactaggct tttgcaaaaa gctccggatc gatcctgaga
     2161 acttcagggt gagtttgggg acccttgatt gttctttctt tttcgctatt gtaaaattca
     2221 tgttatatgg agggggcaaa gttttcaggg tgttgtttag aatgggaaga tgtcccttgt
     2281 atcaccatgg accctcatga taattttgtt tctttcactt tctactctgt tgacaaccat
     2341 tgtctcctct tattttcttt tcattttctg taactttttc gttaaacttt agcttgcatt
     2401 tgtaacgaat ttttaaattc acttttgttt atttgtcaga ttgtaagtac tttctctaat
     2461 cacttttttt tcaaggcaat cagggtatat tatattgtac ttcagcacag ttttagagaa
     2521 caattgttat aattaaatga taaggtagaa tatttctgca tataaattct ggctggcgtg
     2581 gaaatattct tattggtaga aacaactaca tcctggtcat catcctgcct ttctctttat
     2641 ggttacaatg atatacactg cttgagatga ggataaaata ctctgagtcc aaaccgggcc
     2701 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     2761 ttattgtgct gtctcatcat tttggcaaag aattgtaata cgactcacta tagggcgaat
     2821 tcaagcttga tttaggtgac actatagaat acaagctact tgttcttttt gcaggatcca
     2881 ccatgtccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc gatgcaacga
     2941 gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg ttttctgagc
     3001 atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc aagttgaata
     3061 accggaaatg gtttcccgca gaacctgaag atgttcgcga ttatcttcta tatcttcagg
     3121 cgcgcggtct ggcagtaaaa actatccagc aacatttggg ccagctaaac atgcttcatc
     3181 gtcggtccgg gctgccacga ccaagtgaca gcaatgctgt ttcactggtt atgcggcgga
     3241 tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca
     3301 ctgatttcga ccaggttcgt tcactcatgg aaaatagcga tcgctgccag gatatacgta
     3361 atctggcatt tctggggatt gcttataaca ccctgttacg tatagccgaa attgccagga
     3421 tcagggttaa agatatctca cgtactgacg gtgggagaat gttaatccat attggcagaa
     3481 cgaaaacgct ggttagcacc gcaggtgtag agaaggcact tagcctgggg gtaactaaac
     3541 tggtcgagcg atggatttcc gtctctggtg tagctgatga tccgaataac tacctgtttt
     3601 gccgggtcag aaaaaatggt gttgccgcgc catctgccac cagccagcta tcaactcgcg
     3661 ccctggaagg gatttttgaa gcaactcatc gattgattta cggcgctaag gatgactctg
     3721 gtcagagata cctggcctgg tctggacaca gtgcccgtgt cggagccgcg cgagatatgg
     3781 cccgcgctgg agtttcaata ccggagatca tgcaagctgg tggctggacc aatgtaaata
     3841 ttgtcatgaa ctatatccgt aacctggata gtgaaacagg ggcaatggtg cgcctgctgg
     3901 aagatggcga tgaattcaag ttggaattgg tagaaaaact ttttgctgaa gacacagaag
     3961 caaagaaccc attttctact caggacacag atttagactt ggagatgtta gctccctata
     4021 tcccaatgga tgatgacttc cagttacgtt ccttcgatca gttgtcacca ttagaaagca
     4081 gttccgcaag ccctgaaagc gcaagtcctc aaagcacagt tacagtattc cagcagactc
     4141 aaatacaaga acctactgct aatgccacca ctaccactgc caccactgat gaattaaaaa
     4201 cagtgacaaa agaccgtatg gaagacatta aaatattgat tgcatctcca tctcctaccc
     4261 acatacataa agaaactact tagcctagtg ccatttgttc agtggttcgt agggctttcc
     4321 cccactgttt ggctttcagt tatatggatg atgtggtatt gggggccaag tctgtacagc
     4381 atcttgagtc cctttttacc gctgttacca attttctttt gtctttgggt atacatttaa
     4441 accctaacaa aacaaagaga tggggttact ctctaaattt tatgggttat gtcattggat
     4501 gttatgggtc cttgccacaa gaacacatca tacaaaaaat caaagaatgt tttagaaaac
     4561 ttcctattaa caggcctatt gattggaaag tatgtcaacg aattgtgggt cttttgggtt
     4621 ttgctgcccc ttttacacaa tgtggttatc ctgcgttgat gcctttgtat gcatgtattc
     4681 aatctaagca ggctttcact ttctcgccaa cttacaaggc ctttctgtgt aaacaatacc
     4741 tgaaccttta ccccgttgcc cggcaacggc caggtctgtg ccaagtgttt gctgacgcaa
     4801 cccccactgg ctggggcttg gtcatgggcc atcagcgcat gcgtggaacc ttttcggctc
     4861 ctctgccgat ccatactgcg gaactcctag ccgcttgttt tgctcgcagc aggtctggag
     4921 caaacattat cgggactgat aactctgttg tcctatcccg caaatataca tcgtttccat
     4981 ggctgctagg ctgtgctgcc aactggatcc tgcgcgggac gtcctttgtt tacgtcccgt
     5041 cggcgctgaa tcctgcggac gacccttctc ggggtcgctt gggactctct cgtccccttc
     5101 tccgtctgcc gttccgaccg accacggggc gcacctctct ttacgcggac tccccgtctg
     5161 tgccttctca tctgccggac cgtgtgcact tcgcttcacc tctgcacgtc gcatggagac
     5221 caccgtgaac gcccaccaaa tattgcccaa ggtcttacat aagaggactc ttggactctc
     5281 agcaatgtca acgaccgacc ttgaggcata cttcaaagac tgtttgttta aagactggga
     5341 ggagttgggg gaggagatta ggttaaaggt ctttgtacta ggaggctgta ggcataaatt
     5401 ggtctgcgca ccagcaccat ggcgcaatca ctagagcggg gtacctttaa gaccaatgac
     5461 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5521 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     5581 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5641 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5701 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     5761 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     5821 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5881 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5941 tgtctggctc tagctatccc gcccctaact ccgcccattc tccgccccat ggctgactaa
     6001 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6061 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     6121 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     6181 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     6241 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     6301 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6361 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6421 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6481 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6541 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6601 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6661 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     6721 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     6781 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6841 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6901 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     6961 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7021 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7081 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7141 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7201 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7261 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7321 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7381 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7441 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7501 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7561 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7621 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7681 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7741 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7801 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7861 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7921 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7981 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8041 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8101 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8161 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8221 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8281 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8341 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8401 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8461 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8521 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8581 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8641 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     8701 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     8761 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     8821 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     8881 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     8941 aagctggagc tgcaagctta a
//
