LOCUS       sequence-305950-        1439 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cassette of 10 different binding sites with high affinity to PP7 
            phage coat protein, as extracted from the oligo pool experiment..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Katz N, Tripto E, Granik N, Goldberg S, Atar O, Yakhini Z, Orenstein
            Y, Amit R
  TITLE     Overcoming the design, build, test bottleneck for synthesis of 
            nonrepetitive protein-RNA cassettes.
  JOURNAL   Nat Commun. 2021 Mar 11;12(1):1576. doi: 10.1038/s41467-021-21578-6.
  PUBMED    33707432
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-11"; volume: "12(1):1576. doi"; pages: " 
            10.1038/s41467-021-21578-6"
COMMENT     Sequence Label: CMV-10xPP7 oligo-library based
FEATURES             Location/Qualifiers
     source          1..1439
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        25..69
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     polyA_signal    633..754
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(644..663)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     698..717
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1009..1026)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acgtacactc cgcccattga cgcaatgggc ggtaggcgtg tacggtggga ggtctatata
       61 agcagagctg gtttatgaac cgtcagatcc gagctcctat ctagcgtcgc gttgacgaat
      121 tcttacatat ggagtttata tggaaaccca tacgcctgct ggtcgtgacc agagatttca
      181 tatgggaaac tctgcgagac aatgctacct taccgtcggc ccacttgttt ttacccatga
      241 ttccagttta tatggaaacg gaatcgcctg ctggtcgtga ctaaggagtt tatatggaaa
      301 cccttagaaa agccgtcgcc ttgaagccga gaacaaaagg agattatatg aaatcccttt
      361 atggattggg tctccaattc ctgtctgtta cgagtcaata tggtgaccgt acatcaggct
      421 tcgcagtatg caacgcttgc gtaagcacat tataaggaat ggcttattca ccgctttcct
      481 aagattggag taaatatggt tacccaatgt actaactcgc agtttcgaca ttatatggaa
      541 tgcgaaacag aaacgtcacg tcctggctac ggagtttata tggaaacccg taggaagaat
      601 tccccctaag cttccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
      661 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      721 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
      781 gtttcaggtt cagggggagg tgttgggaag gttttttaaa gcagttaaaa tctctacaaa
      841 tgtggtaaga tccataagga tcgagtatgt caaccgatgc ccttgagagc cttcaaccca
      901 gtcatctcct tccggtgagc accgggcatg actatcatca ccgcacttat gactgtcgtc
      961 cgtagcaggc cactcgtacg acaggtaccg acatcgctct tccgcttcct cgctcactga
     1021 ctcgctgcgc tcggtcgttc ggctgcggca agcggtatca cctcactcaa aggcagtaat
     1081 acggttatcg acagaatcat gtggacatct catgaaggaa ctgtcgaccc aaaggccagc
     1141 aaaaagccag taaccgtaaa aaggccccgt gctggcgttt ttccaaagcc cccccccccc
     1201 ttactaacat ccaaaaatca cccctgaatt ccaaagtggc caaacccgaa gggctaagga
     1261 agcccggctt tttcccttga aaatccccgg gggcccttcc tgtgccaccc ctcctttacg
     1321 gaaacatatc cccttttccc tctggagagg gggaatttta aaaagagggg ggggaatttt
     1381 agttccgggg gggtgtaagc gggggttgtg acaacccccg ttttaaagaa gaatataag
//
