LOCUS       sequence-305953-        1477 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cassette of 10 different binding sites with high affinity to MS2 
            phage coat protein, as extracted from the oligo pool experiment..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Katz N, Tripto E, Granik N, Goldberg S, Atar O, Yakhini Z, Orenstein
            Y, Amit R
  TITLE     Overcoming the design, build, test bottleneck for synthesis of 
            nonrepetitive protein-RNA cassettes.
  JOURNAL   Nat Commun. 2021 Mar 11;12(1):1576. doi: 10.1038/s41467-021-21578-6.
  PUBMED    33707432
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-11"; volume: "12(1):1576. doi"; pages: " 
            10.1038/s41467-021-21578-6"
COMMENT     Sequence Label: CMV-10xMS2 oligo-library based
FEATURES             Location/Qualifiers
     source          1..1477
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    589..710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(600..619)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     654..673
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(966..983)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccgtaccact tcccgcccca tttgacgcaa tgggcggtta ggcgttgtta cggtgggaag
       61 gttcctatat aagcagaagc tggtttatga accgtcagag accgagctcc tatctggcgt
      121 cgcgttgacg aattcttaca tgaagacgat tacgcttcac gcctgctggt cgtgacagtt
      181 gaccattagg caactcgaga caatgctacc ttaccgtcgg cccacttgtt tttacccatg
      241 agaacacgat tacggttctc gcctgctggt cgtgacacat gaggattacc catgtgaaac
      301 agccgtcgcc ttgaagccga gaacaacttg accattaggc aagtatggat tgggtctcca
      361 attcctgtct gtagatcacc attagggatc tcatcaggct tcgcagtatg caacgcttgc
      421 gatgcgagga ttacccgcat ttcaccgctt tcctaagagt tcagcattag cgaactgtac
      481 taactcgcag ttgtgaggag tacccacaac agaaacgtca cgtcctggca catgaggatt
      541 acccatgtgg aagaattccc cctaagcttc cgcttcgagc agacatgata agatacattg
      601 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      661 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
      721 attgcattca ttttatgttt caggtcaggg ggaggtgttg tggaaagttt ttttaaacca
      781 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcg agtatgtcga ccgatgccct
      841 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
      901 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc
      961 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     1021 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1081 tgagcaaaag gccagccgaa ggccaggaaa ccgtaaaaaa ggccccgttg ctggcgtttt
     1141 ttccataggc tccccccccc ctgacaaaca tcacaaaaaa tcgaaccctc aattccaaag
     1201 gtggccaaaa cccggacagg aactataaaa gatacccagg cgttttcccc cctggaaaac
     1261 tcccctcctt gcccctcccc tgtttccaac ccccgccccc tttccggaaa cccgtccccc
     1321 cttttttccc ttcggggaaa ggtggggctt tttccaaacc cccccttttt aagttttccc
     1381 cttttcgggt gtgcttttcc ccccccccgg ggggggtgtg ttggccacac ccccccccct
     1441 ttttccccac ccccgccccc tttcccgaaa attaatt
//
