LOCUS       sequence-305955-        1384 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cassette of 10 different binding sites with high affinity to QB 
            phage coat protein, as extracted from the oligo pool experiment..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Katz N, Tripto E, Granik N, Goldberg S, Atar O, Yakhini Z, Orenstein
            Y, Amit R
  TITLE     Overcoming the design, build, test bottleneck for synthesis of 
            nonrepetitive protein-RNA cassettes.
  JOURNAL   Nat Commun. 2021 Mar 11;12(1):1576. doi: 10.1038/s41467-021-21578-6.
  PUBMED    33707432
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-11"; volume: "12(1):1576. doi"; pages: " 
            10.1038/s41467-021-21578-6"
COMMENT     Sequence Label: CMV-10xQb oligo-library based
FEATURES             Location/Qualifiers
     source          1..1384
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        25..70
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     polyA_signal    595..716
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(606..625)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     660..679
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ccgtacactc cgcccattga cgcaatgggc ggtaggcgtg tacggtggga ggtcctatat
       61 aagcagagct ggtttatgaa ccgtcagatc cgagctccta tctagcgtcg cgttgacgaa
      121 ttcttacaat gcatgtctaa gacagcatct cgtgctgatc gtgacttgga tgtccaagac
      181 accaacgaga caatgctacc ttaccgtcgg cccacttgtt tttacccatg acatgacgag
      241 atactcgcat gtcgcctgct ggtcgtgact aaggtgttta aggttaaacc cttagaaaca
      301 gccgtcgcct tgaagccgag aacattgcat gtcgaagaca gcaaatggat tgggtctcca
      361 attcctgtct gtatgcatgt ctaagacagc atcatcaggc ttcgcagtat gcaacgcttg
      421 cgatgcattg caaagcaagc atttcaccgc tttcctaaga aggatagtaa tgactacctt
      481 gtactaactc gcagatcgaa ctctaagagt cgatcagaaa cgtcacgtcc tggcatgcat
      541 gtctaagaca gcatggaaga attcccccta agcttccgct tcgagcagac atgataagat
      601 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
      661 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
      721 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggga ggttttttaa
      781 aacaactgga agctcaacca catgcggtaa aatcgatatc gaatcaagta tgtgcagcgg
      841 tgcgcttgga aagcgtcggg ggagtagctc tttatgatga tagcagagaa ttaaaaatag
      901 tcgaagggat tataaatgac tatttatgta gcacaatgag gaacacttcc aaccacgctc
      961 tactgattct cgagtacatg actcggttcg ctacatctcg ctaatgcgaa gcacggaaga
     1021 actaatacac atcctttaca cctctagccc cgaatcatgc aacacaccgc agcctccaga
     1081 aacccagcac acaaaaagcg ggtaaaagaa aaaagccacg aaggtgtact taacgaggtt
     1141 cgcccaccca gacaagagac cggcatatgc catgtccgta gtggggggaa gaagcggcga
     1201 acgcgggtca gggagtaaaa cgagaaagct agctggtgtt ttcttttgaa aagggataac
     1261 agcgagcccg cgctgaggcc tcggcgtgag gatgaatccg atggtggaga tggacgtggt
     1321 aggatgagtt tggccccccg ccgtggaccg acgtattaac ttttcgtggt cgttccagat
     1381 tacc
//
