LOCUS       sequence-305963-        1437 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cassette of 10 different binding sites with high affinity to both 
            PP7 and Qb phage coats protein, as computed by the ML model..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Katz N, Tripto E, Granik N, Goldberg S, Atar O, Yakhini Z, Orenstein
            Y, Amit R
  TITLE     Overcoming the design, build, test bottleneck for synthesis of 
            nonrepetitive protein-RNA cassettes.
  JOURNAL   Nat Commun. 2021 Mar 11;12(1):1576. doi: 10.1038/s41467-021-21578-6.
  PUBMED    33707432
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-11"; volume: "12(1):1576. doi"; pages: " 
            10.1038/s41467-021-21578-6"
COMMENT     Sequence Label: CMV-10xPP7-Qb model based
FEATURES             Location/Qualifiers
     source          1..1437
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        26..71
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     polyA_signal    590..711
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(601..620)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     655..674
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(969..986)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggtacactc cgcccatttg acgcaatggg cggtaggcgt gtacggtggg aggtcctata
       61 taagcagagc tggtttatga accgtcagat ccgagctcct atctagcgtc gcgttgacga
      121 attctacgat gcaacctaag acatcccggc caggacgtga cgtttctgct gttgtctccg
      181 acatcccgct gcgagttagt acttgcagtc taaaacatcc cgcttaggaa agcggtgaat
      241 ggctgtcttc tacatccggc gcaagcgttg catactgcga agcctgatga tgctcacttc
      301 tacatcccga cagacaggaa ttggagaccc aatccatacg ctgtctacta catcccatgt
      361 tctcggcttc aaggcgacgg ctgtttcatg cttactcaga catcgtcgtc acgaccagca
      421 ggcgaagctg tctaatacat catacatggg taaaaacaag tgggcccgac cggtaaggta
      481 gcattgtctc gatgatgtct aagtcatctc cgtcacgacc agcaggcgat gcagtctaag
      541 ccatatcgtg taagaattcc ccctaagctt ccgcttcgag cagacatgat aagatacatt
      601 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
      661 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
      721 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggggaaagg tttttttaaa
      781 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga ttgagtatgt cgaccgatgc
      841 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
      901 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
      961 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1021 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1081 atggtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1141 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa ttcaaaggtg
     1201 gcgaaacccc gacaggaata ttaaggatac caggcctttc cccccggaaa cctccctcgg
     1261 gggctctcct gtttccaacc ctgcccttta cggaaaactt ttcgcctttt cccctttggg
     1321 aaaggggggg gctttttccc aaccccaccc gtggaggttt ttccattttg gggggaggtg
     1381 ctttcccccc ccacgagggg gggttgggga aaaaaacccc cctttaaccg caaaggg
//
