LOCUS       sequence-305966-        1503 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cassette of 10 different binding sites with high affinity to MS2 
            phage coat protein, as extracted form the oligo pool experiment. It 
            does not contain the MS2-wt sequence, therefore QCP cannot bind it..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Katz N, Tripto E, Granik N, Goldberg S, Atar O, Yakhini Z, Orenstein
            Y, Amit R
  TITLE     Overcoming the design, build, test bottleneck for synthesis of 
            nonrepetitive protein-RNA cassettes.
  JOURNAL   Nat Commun. 2021 Mar 11;12(1):1576. doi: 10.1038/s41467-021-21578-6.
  PUBMED    33707432
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-11"; volume: "12(1):1576. doi"; pages: " 
            10.1038/s41467-021-21578-6"
COMMENT     Sequence Label: CMV-10xMS2 no wild type
FEATURES             Location/Qualifiers
     source          1..1503
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    583..704
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(594..613)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     648..667
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(962..979)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctgtacactt ccgctccatt tgacgcaatg ggcggttagg cgttgtacgg tgggaaggtc
       61 tatataagca gagctggttt atgaaccgtc agagaccgag ctcctatcta gcgtcgcgtt
      121 gacgaattct tacatgaaga cgattacgct tcacgcctgc tggtcgtgac agttgaccat
      181 taggcaactc gagacaatgc taccttaccg tcggcccact tgtttttacc catgagaaca
      241 cgattacggt tctcgcctgc tggtcgtgac agctgacgaa tacgcagctg aaacagccgt
      301 cgccttgaag ccgagaacaa cttgaccatt aggcaagtat ggattgggtc tccaattcct
      361 gtctgtagat caccattagg gatctcatca ggcttcgcag tatgcaacgc ttgcgatgcg
      421 aggattaccc gcatttcacc gctttcctaa gagttcagca ttagcgaact gtactaactc
      481 gcagttgtga ggagtaccca caacagaaac gtcacgtcct ggcagaagag cattagcctt
      541 ctggaagaat tccccctaag cttccgcttc gagcagacat gataagatac attgatgagt
      601 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      661 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      721 ttcattttat gtttcaggtc agggggaggg gtgggggggg ggttttttaa acccaattaa
      781 aacctctaca aatgtggtaa aatccaataa ggatcgagta tgtcgaccga tgcccttgag
      841 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
      901 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt
      961 gctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1021 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1081 caaaaggcca gcaaaaggcc caggaaccgt aaaaaggccg cgtttgctgg cgttttttcc
     1141 ctaaggctcc gccccccctg acgagcatcc caaaaatcga cgcctccagt tccaaaggtg
     1201 ggcgaaaccc cgaccgggac tataaagaat acccaggccg ttttcccccc gggaaacttc
     1261 ccccctggcg ccctccctgt ttccaacccg gccccctttc cgggaaacct tgtccgccct
     1321 ttttcccctt tgggaaaagg gggggccttt ttttctaaac ctcccccccg tgagggtttt
     1381 cttttttggg gggtgggtgc ttttcccccc cccccggggg tgggtgtggg cgaaaacccc
     1441 ccctcttctc ccccccgccc gccccctttt tcttgtaata tattttcttt gtgttccacc
     1501 cgg
//
