LOCUS       sequence-305969-        1473 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cassette of 10 different binding sites with high affinity to Qb 
            phage coat protein, as extracted form the oligo pool experiment. It 
            does not contain the Qb-wt sequence, therefore MCP cannot bind it..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Katz N, Tripto E, Granik N, Goldberg S, Atar O, Yakhini Z, Orenstein
            Y, Amit R
  TITLE     Overcoming the design, build, test bottleneck for synthesis of 
            nonrepetitive protein-RNA cassettes.
  JOURNAL   Nat Commun. 2021 Mar 11;12(1):1576. doi: 10.1038/s41467-021-21578-6.
  PUBMED    33707432
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-11"; volume: "12(1):1576. doi"; pages: " 
            10.1038/s41467-021-21578-6"
COMMENT     Sequence Label: CMV-10xQb no wild type
FEATURES             Location/Qualifiers
     source          1..1473
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    608..729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(619..638)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     673..692
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(986..1003)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctgttaccct tcccgctcca tttgacgcaa tgggcggtta ggcgttgtta cggtgggaag
       61 gtcctatata agcagaagct ggtttatgaa ccgtcagaga ccgagctcct atctagcgtc
      121 gcgttgacga attacttaca aaggaactgt aacagtcctt ctcgtgctga tcgtgacttg
      181 gatgtccaag acaccaacga gacaatgcta ccttaccgtc ggcccacttg tttttaccca
      241 tgacatgacg agatactcgc atgtcgcctg ctggtcgtga ctaaggtgtt taaggttaaa
      301 cccttagaaa cagccgtcgc cttgaagccg agaacattgc atgtcgaaga cagcaaatgg
      361 attgggtctc caattcctgt ctgtttccat gactaagtca ggaacatcag gcttcgcagt
      421 atgcaacgct tgcgatgcat tgcaaagcaa gcatttcacc gctttcctaa gaaggatagt
      481 aatgactacc ttgtactaac tcgcagatcg aactctaaga gtcgatcaga aacgtcacgt
      541 cctggcaacc atgtcaggga caggtttgga agaattcccc ctaagcttcc gcttcgagca
      601 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
      661 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
      721 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgttg
      781 ggaaggtttt tttaaggcaa ttaaaacctt ttacaaatgt ggtaaaattg ataaggatca
      841 agtatgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
      901 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
      961 ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1021 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1081 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaaag
     1141 gccgcgtttg ctggcgtttt ttcctaaggc tccgcccccc tgacgaacat cccaaaaatc
     1201 gacccctcaa ttccaaggtg ggcaaacccc caccggaaca ttaaaaaatc caggcttttc
     1261 ccccctggaa acccccccct ggcgcctccc ctgtttcaac cccgcccgct acccgaaaaa
     1321 cctttttccc ccttttccct tttggggaag ggggggggtt ttttctagct cccccccggg
     1381 agttttctat ttttgggtgg ggtgttctcc ccccccctgg ggctgtgtcg caaaaacccc
     1441 ccttctaccc caccgagccc ccttattaga aaa
//
