LOCUS       sequence-305972-        1324 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Qb coat protein fused to three repeats of the blue fluorescent 
            protein under a Efla promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Katz N, Tripto E, Granik N, Goldberg S, Atar O, Yakhini Z, Orenstein
            Y, Amit R
  TITLE     Overcoming the design, build, test bottleneck for synthesis of 
            nonrepetitive protein-RNA cassettes.
  JOURNAL   Nat Commun. 2021 Mar 11;12(1):1576. doi: 10.1038/s41467-021-21578-6.
  PUBMED    33707432
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-11"; volume: "12(1):1576. doi"; pages: " 
            10.1038/s41467-021-21578-6"
COMMENT     Sequence Label: Ef1a-QCP-3xBFP
FEATURES             Location/Qualifiers
     source          1..1324
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgtcgtgacg cggttccggt taaggtgaac gcgccgccac catggcaaaa cttcaggcaa
       61 ttacgttaag tggtatcggt aagaacggtg atgtcacctt gaatttgaac cctcgtggcg
      121 tcaacccgac gaatggtgtg gctgcgctga gtgaagcggg cgcggtgcct gctttggaaa
      181 aacgtgtcac gatctctgtc tctcaaccga gccgtaatcg taaaaactat aaagtacagg
      241 tcaagatcca aaatcctacc agctgcacgg catccggcac gtgcgatccg agcgtgacgc
      301 gttccgcgta tgcggatgtc acgtttagtt tcacgcaata tagtaccgat gaagaacgcg
      361 cactggttcg cacggaactt aaagcccttt tggctgatcc gatgctgatt gatgcgatcg
      421 ataacctgaa tcctgcctat gctagcctgc agggatccgg aagtatggtg tctaagggcg
      481 aagagctgat taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc
      541 atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga
      601 gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct
      661 tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc
      721 agtccttccc tgagggcttc acatggggag agagtcacca catacgaaga acggggggcg
      781 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcagga
      841 ttcagaaggg gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcgggct
      901 gggaggcctt caccgagacg ctgtaccccg ctgacggcgg gcctggaagg cagaaacgac
      961 atggccctga agctcgtggg cgggagccat ctgatcgcaa acgccaagac cacatataga
     1021 tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg
     1081 gaaagaatca aggaggccaa caacgaaacc tacgtcgagc agcaccaagg tggcagtggc
     1141 cagatactgc gacctcccta gcaaactggg ggcacaagct taatctcgag ccaagaaaaa
     1201 gcggaaatgg tattctagat aatccacctc tggattacaa atttgggaaa gaattgacgg
     1261 ggattcctaa caaggttgcc ccttttaccc aaggggaaac cccgctttaa ggccttgtat
     1321 caag
//
