LOCUS       sequence-30598-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Mills E, Truong K
  TITLE     Rate and extent of protein localization is controlled by 
            peptide-binding domain association kinetics and morphology.
  JOURNAL   Protein Sci. 2009 Jun . 18(6):1252-60.
  PUBMED    19472343
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2009 Jun . 18(6):1252-60."
COMMENT     Sequence Label: VCB1-CKKp
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             140..163
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    217..679
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(273..290)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tcgccgccgc ctgccgccca agaagagggc tagtgtgaaa ctgattccga gctggaccac
       61 cgtgattctg gtgaaatcta tgctgcgtaa acgtagcttc ggcaacccgt tcgctagcct
      121 gtttaaactg taactcgagc accaccatca ccatcaccat cactaagtga ttaacctcag
      181 gtgcaggctg cctatcagaa ggtggtggct ggtgtggagg gcccggaaac ctggccctgt
      241 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
      301 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
      361 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
      421 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
      481 agttgtggaa agagtcaaat ggctcacctc aagcgtattc aacaaggggc tgaaggatgc
      541 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
      601 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
      661 ttgaaaaaca cgatgataag cttgccacaa cccgtaccaa agatggatag atccggaaag
      721 cctgaactca ccgcgacgtc tgttgagaag tttctgatcg
//
