LOCUS       sequence-305980-       13682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, 
            activating DsRed-SKL with promoter 5xQUAS+CP_pcbC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13682)
  AUTHORS   Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
  TITLE     Synthetic control devices for gene regulation in Penicillium 
            chrysogenum.
  JOURNAL   Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 
            10.1186/s12934-019-1253-3.
  PUBMED    31739777
REFERENCE   2  (bases 1 to 13682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2019-11-18"; volume: "18(1):203. doi"; pages: " 
            10.1186/s12934-019-1253-3"
COMMENT     Sequence Label: pLM2_42_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS
FEATURES             Location/Qualifiers
     source          1..13682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2222..2332
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             join(2345..2347,2348..2350,2351..3061)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2345..2347,2348..2350,2351..3061)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2390..2411)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2651..2670)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2998..3019
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3062..3082
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4254..4369
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             6952..7626
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(7082..7100)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(7132..7152)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     7544..7567
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    9460..9484
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      9572..10282
                     /label=oriV
                     /note="incP origin of replication"
     promoter        10303..10407
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10408..11202
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11373..11961
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11862..11881
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12297..13445
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    13591..13615
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gcctgcagga tgggcctcca caaccctgcc ctaacattgt
       61 tcgtagaaga tctgccagca ggcttatgtc caggcccctc tcaacccgcc ctttcctttc
      121 agctcgggcg ccgtacttgc cccacccagg cgtgttccgc tgaactgccc atacccaggc
      181 cagcggactg ataaccattt ctctttatgc tttgctattg ggcttgtgac tcgttcttgg
      241 aggcaaggtt atcagattga atagaagatt cggtgatgca gcaaatagcg actgttcgtt
      301 gcggggtccg aacccgctcg gcagcaccgg gctctcccta ctatccctcg atagcagtgg
      361 cgtgcgcggg ctgtcgaccc tgtccatcct gaaagagttg atattgaagg tagtaggtac
      421 ggtgcagggt aacgcgtgat atcctgtcct cagtcttaag acttctcacc tatatcaccc
      481 gcacttaccc tagagtgccg cttaggtgct aagggcacat tgagtattgg ccgtgtagaa
      541 tatatagctt aagtacggcc aagcagacgg gaagccctgt tctccacacc ctatggtcgt
      601 atatatcagg cttctaccgg gaaacgatta agagtgtata atggactgaa aatcaatatg
      661 aacgggacaa tgctcaagtt aaattagtta ggcatcctaa tctctactaa atgttctatc
      721 tagagatcgg ggtactatag gcccgtacgt taatcactct acgcttctct cccttaggta
      781 tagtgtaggt aggggctaga catttatatg agtcagatgg tacaaacggt agcgctgcaa
      841 gaattcaagc ttggagtctt gcgttacggg cgtattttgc tgcggccggt ggtgcccctc
      901 catgccccgc catctttcaa agctcctggc gacgccgtca tctccgaaca ttctcccccc
      961 aaaggaatca attggcaatt ggagtctagt aaagtggtgt ttgtcatcag taaggagttg
     1021 gtgaaactac aatcttccat catgaagaga agggatattt ttggggttgt attttacgat
     1081 gaaggtactg gaaatggtgg gggtttttat agcagtagac agtcagtcag taagtagtat
     1141 gcttgttgta ttacccaaac cagatcaatc caaagaaagc ctgacagaca gccatcaata
     1201 gatactactt cgtactatag ttacccacct aaccatatta ctcaaaaagc atctatctat
     1261 ccgcgggctt ccatgcatgt cccggtagca aactcctccc accggtgtag tactctttgg
     1321 ttagtagtct tgttcaccgg aggactctgc tcctctcctg ctcaggtgct gccccgccct
     1381 ccgtcccacc atgacggaag agatgctccg taagccgtcc agttgcaacg aatcctgctc
     1441 tgacatcttc gaacgccttc tccctttcgc tcgcttctct gcctctttcc tctcttccct
     1501 ttccttcccc tccaaactaa accttcctcc ttttctccat catcctctag gcagttggtt
     1561 cttcctgact gtacatatat ccaccacctc ccccctctat tcttccacct cttccatatc
     1621 tccttctcca gagttcatac cccccacaat gccacccaag cgcaaaacgc ttaacgctgc
     1681 ggctgaggct aacgctcatg ccgacggaca cgccgacgga aacgccgacg gacacgtggc
     1741 caatacggcc gcgtcctcga ataatgcgag gttcgctgat ctcactaaca tcgatactcc
     1801 gggtctggga cccacaacta cgaccctgct cgtggaacca gcacgctcaa agcgtcaacg
     1861 agtgtcccgc gcatgcgacc agtgccgtgc agcccgagag aaatgcgacg gaatacagcc
     1921 tgcgtgtttc ccgtgcgttt cccagggaag gtcctgcact tatcaggctt cgccgaaaaa
     1981 gaggggagtt caaaccggtt atattcgtac gctggagctc gccctcgcct ggatgtttga
     2041 aaatgtcgcg cgttccgaag atgccttgca taacctcctc gtccgtgacg ccggacaagg
     2101 atcagctctg ctcgttggta aagattcgcc ggctgccgag cgactccatg cccgttgggc
     2161 tactagccgt gtcaataaga gcattacccg cctcctcagg tttgaaagcg gcgggccggc
     2221 cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga
     2281 ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggtt
     2341 cgttatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2401 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2461 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     2521 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     2581 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2641 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2701 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2761 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     2821 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     2881 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     2941 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3001 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3061 gccaaagaag aagcggaagg tctaagctta cttctttatc ggttctctct tacgactttt
     3121 tgaatggaac gtttccttct tctcaggcgg gcctatcttt gggccgaagc tcttttcctt
     3181 gtactgtagg acctggttga taatgattcc caaaaagaca tccagcatgt cagttacttg
     3241 cattcgtcag tctatacaaa agcaatggtt tagagaaatt ttgaacttta tacatggttt
     3301 tatttgttgc ttcacggccg taccttctgg aaatccacgg taggagtgtc aatttgcgtt
     3361 tttgataatc cttccaaggt tcttctcgaa gtagttgttc tataattgct tcacagctac
     3421 catggaacat cccaagcacc gctactagaa ttcgagctcg gagtggatcc cccgggctgc
     3481 aggaattcga gctctgtaca gtgaccggtg actctttctg gcatgcggag agacggacgg
     3541 acgcagagag aagggctgag taataagcgc cactgcgcca gacagctctg gcggctctga
     3601 ggtgcagtgg atgattatta atccgggacc ggccgcccct ccgccccgaa gtggaaaggc
     3661 tggtgtgccc ctcgttgacc aagaatctat tgcatcatcg gagaatatgg agcttcatcg
     3721 aatcaccggc agtaagcgaa ggagaatgtg aagccagggg tgtatagccg tcggcgaaat
     3781 agcatgccat taacctaggt acagaagtcc aattgcttcc gatctggtaa aagattcacg
     3841 agatagtacc ttctccgaag taggtagagc gagtacccgg cgcgtaagct ccctaattgg
     3901 cccatccggc atctgtaggg cgtccaaata tcgtgcctct cctgctttgc ccggtgtatg
     3961 aaaccggaaa ggccgctcag gagctggcca gcggcgcaga ccgggaacac aagctggcag
     4021 tcgacccatc cggtgctctg cactcgacct gctgaggtcc ctcagtccct ggtaggcagc
     4081 tttgccccgt ctgtccgccc ggtgtgtcgg cggggttgac aaggtcgttg cgtcagtcca
     4141 acatttgttg ccatattttc ctgctctccc caccagctgc tcttttcttt tctctttctt
     4201 ttcccatctt cagtatattc atcttcccat ccaagaacct ttatttcccc taagtaagta
     4261 ctttgctaca tccatactcc atccttccca tcccttattc ctttgaacct ttcagttcga
     4321 gctttcccac ttcatcgcag cttgactaac agctaccccg cttgagcaga catcaccatg
     4381 cctcaatcct gggaagaact ggccgctgat aagcgcgccc gcctcgcaaa aaccatccct
     4441 gatgaatgga aagtccagac gctgcctgcg gatgacagcg ttattgattt cccaaagaaa
     4501 tcggggatcc tttcagaggc cgaactgaag atcacagagg cctccgctgc agatcttgtg
     4561 tccaagctgg cggccggaga gttgacctcg gtggaagtta cgctagcatt ctgtaaacgg
     4621 gcagcaatcg cccagcagtt agtagggtcc cctctacctc tcagggagat gtaacaacgc
     4681 caccttatgg gactatcaag ctgacgctgg cttctgtgca gacaaactgc gcccacgagt
     4741 tcttccctga cgccgctctc gcgcaggcaa gggaactcga tgaatactac gcaaagcaca
     4801 agacacccgt tggtccactc catggcctcc ccatctctct caaagaccag cttcgagtca
     4861 aggtacaccg ttgcccctaa gtcgttagat gtcccttttt gtcagctaac atatgccacc
     4921 agggctacga aacatcaatg ggctacatct catggctaaa caagtacgac gaaggggact
     4981 cggttctgac aaccatgctc cgcaaagccg gtgccgtttt ctacgtcaag acctctgtcc
     5041 cgcagaccct gatggtctgc gagacagtca acaacatcat cgggcgcacc gtcaacccac
     5101 gcaacaagaa ctggtcgtgc ggcggcagtt ctggtggtga gggtgcgatc gttgggattc
     5161 gtggtggcgt catcggtgta ggaacggata tcggtggctc gattcgagtg ccggccgcgt
     5221 tcaacttcct gtacggtcta aggccgagtc atgggcggct gccgtatgca aagatggcga
     5281 acagcatgga gggtcaggag acggtgcaca gcgttgtcgg gccgattacg cactctgttg
     5341 agggtgagtc cttcgcctct tccttctttt cctgctctat accaggcctc cactgtcctc
     5401 ctttcttgct ttttatacta tatacgagac gggcagtcac tgatgaagta atgttagacc
     5461 tccgcctctt caccaaatcc gtcctcggtc aggagccatg gaaatacgac tccaaggtca
     5521 tccccatgcc ctggcgccag tccgagtcgg acattattgc ctccaagatc aagaacggcg
     5581 ggctcaatat cggctactac aacttcgacg gcaatgtcct tccacaccct cctatcctgc
     5641 gcggcgtgga aaccaccgtc gccgcactcg ccaaagccgg tcacaccgtg accccgtgga
     5701 cgccatacaa gcacgatttc ggccacgatc tcatctccca tatctacgcg gctgacggca
     5761 gcgccgacgt aatgcgcgat atcagtgcat ccggcgagcc ggcgattcca aatatcaaag
     5821 acctactgaa cccgaacatc aaagctgtta acatgaacga gctctgggac acgcatctcc
     5881 agaagtggaa ttaccagatg gagtaccttg agaaatggcg ggaggctgaa gaaaaggccg
     5941 ggaaggaact ggacgccatc atcgcgccga ttacgcctac cgctgcggta cggcatgacc
     6001 agttccggta ctatgggtat gcctctgtga tcaacctgct ggatttcacg agcgtggttg
     6061 ttccggttac ctttgcggat aagaacatcg ataagaagaa tgagagtttc aaggcggtta
     6121 gtgagcttga tgccctcgtg caggaagagt atgatccgga ggcgtaccat ggggcaccgg
     6181 ttgcagtgca ggttatcgga cggagactca gtgaagagag gacgttggcg attgcagagg
     6241 aagtggggaa gttgctggga aatgtggtga ctccataggc ttctaataag tgtcagatag
     6301 caatttgcac aagaaatcaa taccagcaac tgtaaataag cgctgaagtg accatgccat
     6361 gctacgaaag agcagaaaaa aacctgccgt agaaccgaag agatatgaca cgcttccatc
     6421 tctcaaagga agaatccctt cagggttgcg tttccagtct agacacgtat aacggcacaa
     6481 gtgtctctca ccaaatgggt tatatctcaa atgtgatcta aggatggaaa gcccagaata
     6541 ttggctgggt tgatggctgc ttcgagtgca gtctcatgct gccacaggtg actctggatg
     6601 gccccatacc actcaaccca tggtacgagc ggtacgcttt acgaattccc atggggaggg
     6661 gtaatcgatt atcctcggat aaacacttat cccagggtaa tcacttatcc ctggataaac
     6721 aattatcctc gggtaatcgc ttatccccat cgtataatgt ctccaggttg tctcagcata
     6781 aacaccccgc ccccgctcag gcacacagga agagagctca ggtcgtttcc attgcgtcca
     6841 tactcttcac tcattgtcat ctgcaggaga acttcccctg tccctttgcc aagccctctc
     6901 ttcgtcgttg tccacgcctt caagttttca ccattatttt tctagacacc aatggcctcc
     6961 tccgaggacg tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg ctccgtgaac
     7021 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     7081 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     7141 cagttccagt acggctccaa ggtgtacgtg aagcaccccg ccgacatccc cgactacaag
     7201 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     7261 gtggtgaccg tgacccagga ctcctccctg caggacggct ccttcatcta caaggtgaag
     7321 ttcatcggcg tgaacttccc ctccgacggc cccgtaatgc agaagaaaac tatgggctgg
     7381 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccacaag
     7441 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagtccat ctacatggcc
     7501 aagaagcccg tgcagctgcc cggctactac tacgtggact ccaagctgga catcacctcc
     7561 cacaacgagg actacaccat cgtggagcag tacgagcgcg ccgagggccg ccaccacctg
     7621 ttcctgactc acggtatgga cgaattgtac aagtcgaagc tgtaagcttg tgcttctaag
     7681 gtatgagtcg caaaattgtt ttttattttt ggtcttgagt ctaatatgct cgcagctctt
     7741 gcgttgtata tggtcgttgt cgcgtatttt ctgttgtatt aaaagatcaa acgagatcaa
     7801 gggatggctc gcgggctgtc tctcgcacta ggaggaagaa tgcctgaaaa aggaactttg
     7861 attttagctg tggaatagag atggcttgtt tgaggacgct tgtcgcttgg cgcagggact
     7921 tgaatggcag cttgtggaaa ccgaaggcga gaaaagtcga cggatactgt acgtggttct
     7981 attgccagtg cggtggaagc ttccttctca tatagttcaa tccttctttg aatctgtttg
     8041 tttcatattt ggactgtttc attctctgct tgcgcattct catcttcgag aacgactgca
     8101 gggattgttg gttctgtgga gctgatgagc gcgccttgac cacccttgtt cttgttttgc
     8161 tcttttgttc tcatttaacc cgtttctccc ttccaaccct ttgaccttgc aacattgtct
     8221 cccagcgcgt tgccaaagcg aacttgatat cagtatagta tgaccaagta gtctaccaaa
     8281 ataaatttta gtacagtatt gctagttata caagataaat tttgtacaac aatgattatg
     8341 cagagctggt attctgtaag gagtaaattc atgcaggtac agagcgtcca cgtcaggcac
     8401 acgcgcttta gtgctgggta cacattatgc tacctttggg gcaaggtgct attctaggta
     8461 gggtatgcct agcaatgcca tgatcttatg acctcagtaa tctgaccttg gatttaagat
     8521 cggagttctc aaaccctgcg cgctcagaga attcgcatgc ggagacttta gacatacctc
     8581 tcgttgccga caaatcggca ttccgtttcg gtcacggacg taagagttca acaaagccgc
     8641 tcacctgtcc cgttcggcgc ttgagtttcc acttcaaaag cacaaccgct ccgatatttc
     8701 cattgccacc gaccaacaaa aggttcatat cgcgcaatag tcgtccaact ctcactccgc
     8761 ccgcactcga tggcgaagga tggaagaata tccgagcggc atcggataaa gagatctggt
     8821 tcttttcgtg aagtaacgta gggaaccgac ggaaactgta gagctatagg aatttagtat
     8881 atcagaagtc aattgagaga agtgtatagt tagtaactca cttgtgccaa caggtatttc
     8941 caaccgttct tgttcaaatt tcgtcaagtc accatctcta tgtgcaaaac tggatcatac
     9001 aacagctctt attgtagctg catctcgctc tcagagatca tcctatcttc gtcgtcatcc
     9061 accaacacag gtgggagtgc tagttgatcc atgctgaggt caagggacac agacggaatg
     9121 ggtgtagaac gcctcaaaac aggtaaggta tcaggatcgg ttgtccaggg ggaatccaaa
     9181 ccaggttcca ccttgattca gagaataagt atgacgtatt cttggcaatg tttaagcttg
     9241 agatgagccg atgtcaaact gggagacatg atgaaggagg aaatgttgct ttggtggggg
     9301 agacactata aataccaaag agtgaatggt tgataccatt tggggaagga gtgtctatcg
     9361 cagtctctgc agacggcata tcggatagcc tgcaggcgct tgtggaggat gcacatgtga
     9421 ccgagggaca cgaagtgatc cgtttaaact atcagtgttt gacaggatat attggcgggt
     9481 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
     9541 tttatccgtt cgtccatttg tatgtgccag ccgcctttgc gacgctcacc gggctggttg
     9601 ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga
     9661 agccgtgtgc gagacaccgc ggccgccggc gttgtggata cctcgcggaa aacttggccc
     9721 tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt
     9781 tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa
     9841 tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga
     9901 taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat
     9961 ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg
    10021 ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc
    10081 gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg
    10141 gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc
    10201 tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc ggccgcgaac
    10261 ggcctcaccc caaaaatggc agcgctggcc aattcccgag tgcgcggaac ccctatttgt
    10321 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
    10381 cttcaataat attgaaaaag gaagagtatg gctaaaatga gaatatcacc ggaattgaaa
    10441 aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta
    10501 tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa
    10561 gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg
    10621 cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt
    10681 gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt
    10741 atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc
    10801 tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg
    10861 gccgatgtgg attgcgaaaa ctgggaagag gacactccat ttaaagatcc gcgcgagctg
    10921 tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg
    10981 ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga
    11041 agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgct cagggaggat
    11101 atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg
    11161 gagaaaataa aatattatat tttactggat gaattgtttt agctgtcaga ccaagtttac
    11221 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    11281 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    11341 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    11401 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    11461 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    11521 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    11581 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    11641 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    11701 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    11761 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    11821 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    11881 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    11941 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggcagat
    12001 cctagatgtg gcgcaacgat gccggcgaca agcaggagcg caccgacttc ttccgcatca
    12061 agtgttttgg ctctcaggcc gaggcccacg gcaagtattt gggcaagggg tcgctggtat
    12121 tcgtgcaggg caagattcgg aataccaagt acgagaagga cggccagacg gtctacggga
    12181 ccgacttcat tgccgataag gtggattatc tggacaccaa ggcaccaggc gggtcaaatc
    12241 aggaataagg gcacattgcc ccggcgtgag tcggggcaat cccgcaagga gggtgaatga
    12301 atcggacgtt tgaccggaag gcatacaggc aagaactgat cgacgcgggg ttttccgccg
    12361 aggatgccga aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa accttccagt
    12421 ccgtcggctc gatggtccag caagctacgg ccaagatcga gcgcgacagc gtgcaactgg
    12481 ctccccctgc cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt cttgaacagg
    12541 aggcggcagg tttggcgaag tcgatgacca tcgacacgcg aggaactatg acgaccaaga
    12601 agcgaaaaac cgccggcgag gacctggcaa aacaggtcag cgaggccaag caggccgcgt
    12661 tgctgaaaca cacgaagcag cagatcaagg aaatgcagct ttccttgttc gatattgcgc
    12721 cgtggccgga cacgatgcga gcgatgccaa acgacacggc ccgctctgcc ctgttcacca
    12781 cgcgcaacaa gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc cacgtcaaca
    12841 aggacgtgaa gatcacctac accggcgtcg agctgcgggc cgacgatgac gaactggtgt
    12901 ggcagcaggt gttggagtac gcgaagcgca cccctatcgg cgagccgatc accttcacgt
    12961 tctacgagct ttgccaggac ctgggctggt cgatcaatgg ccggtattac acgaaggccg
    13021 aggaatgcct gtcgcgccta caggcgacgg cgatgggctt cacgtccgac cgcgttgggc
    13081 acctggaatc ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc aagaaaacgt
    13141 cccgttgcca ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc gaccactaca
    13201 cgaaattcat atgggagaag taccgcaagc tgtcgccgac ggcccgacgg atgttcgact
    13261 atttcagctc gcaccgggag ccgtacccgc tcaagctgga aaccttccgc ctcatgtgcg
    13321 gatcggattc cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc tgcgaagagt
    13381 tgcgaggcag cggcctggtg gaacacgcct gggtcaatga tgacctggtg cattgcaaac
    13441 gctagggcct tgtggggtca gttccggctg ggggttcagc agcccctgct cggatctgtt
    13501 ggaccggaca gtagtcatgg ttgatgggct gcctgtatcg agtggtgatt ttgtgccgag
    13561 ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt
    13621 gacgcttaga caacttaata acacattgcg gacgttttta atgtactggg gttgaacact
    13681 ct
//
