LOCUS       sequence-305982-       13620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, 
            activating DsRed-SKL with promoter 5xQUAS+CP_ura3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13620)
  AUTHORS   Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
  TITLE     Synthetic control devices for gene regulation in Penicillium 
            chrysogenum.
  JOURNAL   Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 
            10.1186/s12934-019-1253-3.
  PUBMED    31739777
REFERENCE   2  (bases 1 to 13620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2019-11-18"; volume: "18(1):203. doi"; pages: " 
            10.1186/s12934-019-1253-3"
COMMENT     Sequence Label: pLM2_40_pgndA_QfDBD_VP16AD_cp_ura3_DsRed_5xQUAS
FEATURES             Location/Qualifiers
     source          1..13620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2222..2332
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             join(2345..2347,2348..2350,2351..3061)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2345..2347,2348..2350,2351..3061)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2390..2411)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2651..2670)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2998..3019
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3062..3082
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4254..4369
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             6890..7564
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(7020..7038)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(7070..7090)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     7482..7505
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    9398..9422
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      9510..10220
                     /label=oriV
                     /note="incP origin of replication"
     promoter        10241..10345
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10346..11140
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11311..11899
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11800..11819
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12235..13383
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    13529..13553
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gcctgcagga tgggcctcca caaccctgcc ctaacattgt
       61 tcgtagaaga tctgccagca ggcttatgtc caggcccctc tcaacccgcc ctttcctttc
      121 agctcgggcg ccgtacttgc cccacccagg cgtgttccgc tgaactgccc atacccaggc
      181 cagcggactg ataaccattt ctctttatgc tttgctattg ggcttgtgac tcgttcttgg
      241 aggcaaggtt atcagattga atagaagatt cggtgatgca gcaaatagcg actgttcgtt
      301 gcggggtccg aacccgctcg gcagcaccgg gctctcccta ctatccctcg atagcagtgg
      361 cgtgcgcggg ctgtcgaccc tgtccatcct gaaagagttg atattgaagg tagtaggtac
      421 ggtgcagggt aacgcgtgat atcctgtcct cagtcttaag acttctcacc tatatcaccc
      481 gcacttaccc tagagtgccg cttaggtgct aagggcacat tgagtattgg ccgtgtagaa
      541 tatatagctt aagtacggcc aagcagacgg gaagccctgt tctccacacc ctatggtcgt
      601 atatatcagg cttctaccgg gaaacgatta agagtgtata atggactgaa aatcaatatg
      661 aacgggacaa tgctcaagtt aaattagtta ggcatcctaa tctctactaa atgttctatc
      721 tagagatcgg ggtactatag gcccgtacgt taatcactct acgcttctct cccttaggta
      781 tagtgtaggt aggggctaga catttatatg agtcagatgg tacaaacggt agcgctgcaa
      841 gaattcaagc ttggagtctt gcgttacggg cgtattttgc tgcggccggt ggtgcccctc
      901 catgccccgc catctttcaa agctcctggc gacgccgtca tctccgaaca ttctcccccc
      961 aaaggaatca attggcaatt ggagtctagt aaagtggtgt ttgtcatcag taaggagttg
     1021 gtgaaactac aatcttccat catgaagaga agggatattt ttggggttgt attttacgat
     1081 gaaggtactg gaaatggtgg gggtttttat agcagtagac agtcagtcag taagtagtat
     1141 gcttgttgta ttacccaaac cagatcaatc caaagaaagc ctgacagaca gccatcaata
     1201 gatactactt cgtactatag ttacccacct aaccatatta ctcaaaaagc atctatctat
     1261 ccgcgggctt ccatgcatgt cccggtagca aactcctccc accggtgtag tactctttgg
     1321 ttagtagtct tgttcaccgg aggactctgc tcctctcctg ctcaggtgct gccccgccct
     1381 ccgtcccacc atgacggaag agatgctccg taagccgtcc agttgcaacg aatcctgctc
     1441 tgacatcttc gaacgccttc tccctttcgc tcgcttctct gcctctttcc tctcttccct
     1501 ttccttcccc tccaaactaa accttcctcc ttttctccat catcctctag gcagttggtt
     1561 cttcctgact gtacatatat ccaccacctc ccccctctat tcttccacct cttccatatc
     1621 tccttctcca gagttcatac cccccacaat gccacccaag cgcaaaacgc ttaacgctgc
     1681 ggctgaggct aacgctcatg ccgacggaca cgccgacgga aacgccgacg gacacgtggc
     1741 caatacggcc gcgtcctcga ataatgcgag gttcgctgat ctcactaaca tcgatactcc
     1801 gggtctggga cccacaacta cgaccctgct cgtggaacca gcacgctcaa agcgtcaacg
     1861 agtgtcccgc gcatgcgacc agtgccgtgc agcccgagag aaatgcgacg gaatacagcc
     1921 tgcgtgtttc ccgtgcgttt cccagggaag gtcctgcact tatcaggctt cgccgaaaaa
     1981 gaggggagtt caaaccggtt atattcgtac gctggagctc gccctcgcct ggatgtttga
     2041 aaatgtcgcg cgttccgaag atgccttgca taacctcctc gtccgtgacg ccggacaagg
     2101 atcagctctg ctcgttggta aagattcgcc ggctgccgag cgactccatg cccgttgggc
     2161 tactagccgt gtcaataaga gcattacccg cctcctcagg tttgaaagcg gcgggccggc
     2221 cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga
     2281 ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggtt
     2341 cgttatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2401 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2461 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     2521 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     2581 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2641 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2701 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2761 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     2821 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     2881 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     2941 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3001 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3061 gccaaagaag aagcggaagg tctaagctta cttctttatc ggttctctct tacgactttt
     3121 tgaatggaac gtttccttct tctcaggcgg gcctatcttt gggccgaagc tcttttcctt
     3181 gtactgtagg acctggttga taatgattcc caaaaagaca tccagcatgt cagttacttg
     3241 cattcgtcag tctatacaaa agcaatggtt tagagaaatt ttgaacttta tacatggttt
     3301 tatttgttgc ttcacggccg taccttctgg aaatccacgg taggagtgtc aatttgcgtt
     3361 tttgataatc cttccaaggt tcttctcgaa gtagttgttc tataattgct tcacagctac
     3421 catggaacat cccaagcacc gctactagaa ttcgagctcg gagtggatcc cccgggctgc
     3481 aggaattcga gctctgtaca gtgaccggtg actctttctg gcatgcggag agacggacgg
     3541 acgcagagag aagggctgag taataagcgc cactgcgcca gacagctctg gcggctctga
     3601 ggtgcagtgg atgattatta atccgggacc ggccgcccct ccgccccgaa gtggaaaggc
     3661 tggtgtgccc ctcgttgacc aagaatctat tgcatcatcg gagaatatgg agcttcatcg
     3721 aatcaccggc agtaagcgaa ggagaatgtg aagccagggg tgtatagccg tcggcgaaat
     3781 agcatgccat taacctaggt acagaagtcc aattgcttcc gatctggtaa aagattcacg
     3841 agatagtacc ttctccgaag taggtagagc gagtacccgg cgcgtaagct ccctaattgg
     3901 cccatccggc atctgtaggg cgtccaaata tcgtgcctct cctgctttgc ccggtgtatg
     3961 aaaccggaaa ggccgctcag gagctggcca gcggcgcaga ccgggaacac aagctggcag
     4021 tcgacccatc cggtgctctg cactcgacct gctgaggtcc ctcagtccct ggtaggcagc
     4081 tttgccccgt ctgtccgccc ggtgtgtcgg cggggttgac aaggtcgttg cgtcagtcca
     4141 acatttgttg ccatattttc ctgctctccc caccagctgc tcttttcttt tctctttctt
     4201 ttcccatctt cagtatattc atcttcccat ccaagaacct ttatttcccc taagtaagta
     4261 ctttgctaca tccatactcc atccttccca tcccttattc ctttgaacct ttcagttcga
     4321 gctttcccac ttcatcgcag cttgactaac agctaccccg cttgagcaga catcaccatg
     4381 cctcaatcct gggaagaact ggccgctgat aagcgcgccc gcctcgcaaa aaccatccct
     4441 gatgaatgga aagtccagac gctgcctgcg gatgacagcg ttattgattt cccaaagaaa
     4501 tcggggatcc tttcagaggc cgaactgaag atcacagagg cctccgctgc agatcttgtg
     4561 tccaagctgg cggccggaga gttgacctcg gtggaagtta cgctagcatt ctgtaaacgg
     4621 gcagcaatcg cccagcagtt agtagggtcc cctctacctc tcagggagat gtaacaacgc
     4681 caccttatgg gactatcaag ctgacgctgg cttctgtgca gacaaactgc gcccacgagt
     4741 tcttccctga cgccgctctc gcgcaggcaa gggaactcga tgaatactac gcaaagcaca
     4801 agacacccgt tggtccactc catggcctcc ccatctctct caaagaccag cttcgagtca
     4861 aggtacaccg ttgcccctaa gtcgttagat gtcccttttt gtcagctaac atatgccacc
     4921 agggctacga aacatcaatg ggctacatct catggctaaa caagtacgac gaaggggact
     4981 cggttctgac aaccatgctc cgcaaagccg gtgccgtttt ctacgtcaag acctctgtcc
     5041 cgcagaccct gatggtctgc gagacagtca acaacatcat cgggcgcacc gtcaacccac
     5101 gcaacaagaa ctggtcgtgc ggcggcagtt ctggtggtga gggtgcgatc gttgggattc
     5161 gtggtggcgt catcggtgta ggaacggata tcggtggctc gattcgagtg ccggccgcgt
     5221 tcaacttcct gtacggtcta aggccgagtc atgggcggct gccgtatgca aagatggcga
     5281 acagcatgga gggtcaggag acggtgcaca gcgttgtcgg gccgattacg cactctgttg
     5341 agggtgagtc cttcgcctct tccttctttt cctgctctat accaggcctc cactgtcctc
     5401 ctttcttgct ttttatacta tatacgagac gggcagtcac tgatgaagta atgttagacc
     5461 tccgcctctt caccaaatcc gtcctcggtc aggagccatg gaaatacgac tccaaggtca
     5521 tccccatgcc ctggcgccag tccgagtcgg acattattgc ctccaagatc aagaacggcg
     5581 ggctcaatat cggctactac aacttcgacg gcaatgtcct tccacaccct cctatcctgc
     5641 gcggcgtgga aaccaccgtc gccgcactcg ccaaagccgg tcacaccgtg accccgtgga
     5701 cgccatacaa gcacgatttc ggccacgatc tcatctccca tatctacgcg gctgacggca
     5761 gcgccgacgt aatgcgcgat atcagtgcat ccggcgagcc ggcgattcca aatatcaaag
     5821 acctactgaa cccgaacatc aaagctgtta acatgaacga gctctgggac acgcatctcc
     5881 agaagtggaa ttaccagatg gagtaccttg agaaatggcg ggaggctgaa gaaaaggccg
     5941 ggaaggaact ggacgccatc atcgcgccga ttacgcctac cgctgcggta cggcatgacc
     6001 agttccggta ctatgggtat gcctctgtga tcaacctgct ggatttcacg agcgtggttg
     6061 ttccggttac ctttgcggat aagaacatcg ataagaagaa tgagagtttc aaggcggtta
     6121 gtgagcttga tgccctcgtg caggaagagt atgatccgga ggcgtaccat ggggcaccgg
     6181 ttgcagtgca ggttatcgga cggagactca gtgaagagag gacgttggcg attgcagagg
     6241 aagtggggaa gttgctggga aatgtggtga ctccataggc ttctaataag tgtcagatag
     6301 caatttgcac aagaaatcaa taccagcaac tgtaaataag cgctgaagtg accatgccat
     6361 gctacgaaag agcagaaaaa aacctgccgt agaaccgaag agatatgaca cgcttccatc
     6421 tctcaaagga agaatccctt cagggttgcg tttccagtct agacacgtat aacggcacaa
     6481 gtgtctctca ccaaatgggt tatatctcaa atgtgatcta aggatggaaa gcccagaata
     6541 ttggctgggt tgatggctgc ttcgagtgca gtctcatgct gccacaggtg actctggatg
     6601 gccccatacc actcaaccca tggtacgagc ggtacgcttt acgaattccc atggggaggg
     6661 gtaatcgatt atcctcggat aaacacttat cccagggtaa tcacttatcc ctggataaac
     6721 aattatcctc gggtaatcgc ttatccccat cagaaggaag aacgaaggaa ggagcacaga
     6781 cttagattgg tatatatacg catatgtggt gttgaagaaa catgaaattg cccagtattc
     6841 ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcaa tggcctcctc
     6901 cgaggacgtc atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg
     6961 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     7021 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca
     7081 gttccagtac ggctccaagg tgtacgtgaa gcaccccgcc gacatccccg actacaagaa
     7141 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     7201 ggtgaccgtg acccaggact cctccctgca ggacggctcc ttcatctaca aggtgaagtt
     7261 catcggcgtg aacttcccct ccgacggccc cgtaatgcag aagaaaacta tgggctggga
     7321 ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccacaaggc
     7381 cctgaagctg aaggacggcg gccactacct ggtggagttc aagtccatct acatggccaa
     7441 gaagcccgtg cagctgcccg gctactacta cgtggactcc aagctggaca tcacctccca
     7501 caacgaggac tacaccatcg tggagcagta cgagcgcgcc gagggccgcc accacctgtt
     7561 cctgactcac ggtatggacg aattgtacaa gtcgaagctg taagcttgtg cttctaaggt
     7621 atgagtcgca aaattgtttt ttatttttgg tcttgagtct aatatgctcg cagctcttgc
     7681 gttgtatatg gtcgttgtcg cgtattttct gttgtattaa aagatcaaac gagatcaagg
     7741 gatggctcgc gggctgtctc tcgcactagg aggaagaatg cctgaaaaag gaactttgat
     7801 tttagctgtg gaatagagat ggcttgtttg aggacgcttg tcgcttggcg cagggacttg
     7861 aatggcagct tgtggaaacc gaaggcgaga aaagtcgacg gatactgtac gtggttctat
     7921 tgccagtgcg gtggaagctt ccttctcata tagttcaatc cttctttgaa tctgtttgtt
     7981 tcatatttgg actgtttcat tctctgcttg cgcattctca tcttcgagaa cgactgcagg
     8041 gattgttggt tctgtggagc tgatgagcgc gccttgacca cccttgttct tgttttgctc
     8101 ttttgttctc atttaacccg tttctccctt ccaacccttt gaccttgcaa cattgtctcc
     8161 cagcgcgttg ccaaagcgaa cttgatatca gtatagtatg accaagtagt ctaccaaaat
     8221 aaattttagt acagtattgc tagttataca agataaattt tgtacaacaa tgattatgca
     8281 gagctggtat tctgtaagga gtaaattcat gcaggtacag agcgtccacg tcaggcacac
     8341 gcgctttagt gctgggtaca cattatgcta cctttggggc aaggtgctat tctaggtagg
     8401 gtatgcctag caatgccatg atcttatgac ctcagtaatc tgaccttgga tttaagatcg
     8461 gagttctcaa accctgcgcg ctcagagaat tcgcatgcgg agactttaga catacctctc
     8521 gttgccgaca aatcggcatt ccgtttcggt cacggacgta agagttcaac aaagccgctc
     8581 acctgtcccg ttcggcgctt gagtttccac ttcaaaagca caaccgctcc gatatttcca
     8641 ttgccaccga ccaacaaaag gttcatatcg cgcaatagtc gtccaactct cactccgccc
     8701 gcactcgatg gcgaaggatg gaagaatatc cgagcggcat cggataaaga gatctggttc
     8761 ttttcgtgaa gtaacgtagg gaaccgacgg aaactgtaga gctataggaa tttagtatat
     8821 cagaagtcaa ttgagagaag tgtatagtta gtaactcact tgtgccaaca ggtatttcca
     8881 accgttcttg ttcaaatttc gtcaagtcac catctctatg tgcaaaactg gatcatacaa
     8941 cagctcttat tgtagctgca tctcgctctc agagatcatc ctatcttcgt cgtcatccac
     9001 caacacaggt gggagtgcta gttgatccat gctgaggtca agggacacag acggaatggg
     9061 tgtagaacgc ctcaaaacag gtaaggtatc aggatcggtt gtccaggggg aatccaaacc
     9121 aggttccacc ttgattcaga gaataagtat gacgtattct tggcaatgtt taagcttgag
     9181 atgagccgat gtcaaactgg gagacatgat gaaggaggaa atgttgcttt ggtgggggag
     9241 acactataaa taccaaagag tgaatggttg ataccatttg gggaaggagt gtctatcgca
     9301 gtctctgcag acggcatatc ggatagcctg caggcgcttg tggaggatgc acatgtgacc
     9361 gagggacacg aagtgatccg tttaaactat cagtgtttga caggatatat tggcgggtaa
     9421 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
     9481 tatccgttcg tccatttgta tgtgccagcc gcctttgcga cgctcaccgg gctggttgcc
     9541 ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag
     9601 ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc
     9661 actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg cgcggcgttg
     9721 acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc ctcgcaaatc
     9781 ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag cctggggata
     9841 agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg ggcgcgatcc
     9901 ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac atttgagggg
     9961 ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt cggccaccgc
    10021 taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt taaccagggc
    10081 tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc
    10141 ccggcccgct aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg ccgcgaacgg
    10201 cctcacccca aaaatggcag cgctggccaa ttcccgagtg cgcggaaccc ctatttgttt
    10261 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
    10321 tcaataatat tgaaaaagga agagtatggc taaaatgaga atatcaccgg aattgaaaaa
    10381 actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata
    10441 taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg
    10501 gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc
    10561 tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga
    10621 ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat
    10681 cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta
    10741 tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata acgatctggc
    10801 cgatgtggat tgcgaaaact gggaagagga cactccattt aaagatccgc gcgagctgta
    10861 tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg
    10921 agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag
    10981 cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgctca gggaggatat
    11041 cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga
    11101 gaaaataaaa tattatattt tactggatga attgttttag ctgtcagacc aagtttactc
    11161 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    11221 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    11281 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    11341 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    11401 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    11461 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    11521 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    11581 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    11641 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    11701 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    11761 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    11821 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    11881 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggcagatcc
    11941 tagatgtggc gcaacgatgc cggcgacaag caggagcgca ccgacttctt ccgcatcaag
    12001 tgttttggct ctcaggccga ggcccacggc aagtatttgg gcaaggggtc gctggtattc
    12061 gtgcagggca agattcggaa taccaagtac gagaaggacg gccagacggt ctacgggacc
    12121 gacttcattg ccgataaggt ggattatctg gacaccaagg caccaggcgg gtcaaatcag
    12181 gaataagggc acattgcccc ggcgtgagtc ggggcaatcc cgcaaggagg gtgaatgaat
    12241 cggacgtttg accggaaggc atacaggcaa gaactgatcg acgcggggtt ttccgccgag
    12301 gatgccgaaa ccatcgcaag ccgcaccgtc atgcgtgcgc cccgcgaaac cttccagtcc
    12361 gtcggctcga tggtccagca agctacggcc aagatcgagc gcgacagcgt gcaactggct
    12421 ccccctgccc tgcccgcgcc atcggccgcc gtggagcgtt cgcgtcgtct tgaacaggag
    12481 gcggcaggtt tggcgaagtc gatgaccatc gacacgcgag gaactatgac gaccaagaag
    12541 cgaaaaaccg ccggcgagga cctggcaaaa caggtcagcg aggccaagca ggccgcgttg
    12601 ctgaaacaca cgaagcagca gatcaaggaa atgcagcttt ccttgttcga tattgcgccg
    12661 tggccggaca cgatgcgagc gatgccaaac gacacggccc gctctgccct gttcaccacg
    12721 cgcaacaaga aaatcccgcg cgaggcgctg caaaacaagg tcattttcca cgtcaacaag
    12781 gacgtgaaga tcacctacac cggcgtcgag ctgcgggccg acgatgacga actggtgtgg
    12841 cagcaggtgt tggagtacgc gaagcgcacc cctatcggcg agccgatcac cttcacgttc
    12901 tacgagcttt gccaggacct gggctggtcg atcaatggcc ggtattacac gaaggccgag
    12961 gaatgcctgt cgcgcctaca ggcgacggcg atgggcttca cgtccgaccg cgttgggcac
    13021 ctggaatcgg tgtcgctgct gcaccgcttc cgcgtcctgg accgtggcaa gaaaacgtcc
    13081 cgttgccagg tcctgatcga cgaggaaatc gtcgtgctgt ttgctggcga ccactacacg
    13141 aaattcatat gggagaagta ccgcaagctg tcgccgacgg cccgacggat gttcgactat
    13201 ttcagctcgc accgggagcc gtacccgctc aagctggaaa ccttccgcct catgtgcgga
    13261 tcggattcca cccgcgtgaa gaagtggcgc gagcaggtcg gcgaagcctg cgaagagttg
    13321 cgaggcagcg gcctggtgga acacgcctgg gtcaatgatg acctggtgca ttgcaaacgc
    13381 tagggccttg tggggtcagt tccggctggg ggttcagcag cccctgctcg gatctgttgg
    13441 accggacagt agtcatggtt gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
    13501 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga
    13561 cgcttagaca acttaataac acattgcgga cgtttttaat gtactggggt tgaacactct
//
