LOCUS       sequence-305983-       14499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes p40S:QfDBD-QF2AD-GFP-NLS synthetic transcription factor, 
            activating DsRed-SKL with promoter 5xQUAS+CP_nirA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14499)
  AUTHORS   Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
  TITLE     Synthetic control devices for gene regulation in Penicillium 
            chrysogenum.
  JOURNAL   Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 
            10.1186/s12934-019-1253-3.
  PUBMED    31739777
REFERENCE   2  (bases 1 to 14499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2019-11-18"; volume: "18(1):203. doi"; pages: " 
            10.1186/s12934-019-1253-3"
COMMENT     Sequence Label: pLM2_35_p40S_QfDBD_Qf2AD_cp_nirA_DsRed_5xQUAS
FEATURES             Location/Qualifiers
     source          1..14499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(3270..3272,3273..3275,3276..3986)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3270..3272,3273..3275,3276..3986)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3315..3336)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3576..3595)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3923..3944
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3987..4007
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          5179..5294
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             7769..8443
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(7899..7917)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(7949..7969)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     8361..8384
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    10277..10301
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      10389..11099
                     /label=oriV
                     /note="incP origin of replication"
     promoter        11120..11224
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11225..12019
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12190..12778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12679..12698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             13114..14262
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    14408..14432
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gcctgcagga tgggcctcca caaccctgcc ctaacattgt
       61 tcgtagaaga tctgccagca ggcttatgtc caggcccctc tcaacccgcc ctttcctttc
      121 agctcgggcg ccgtacttgc cccacccagg cgtgttccgc tgaactgccc atacccaggc
      181 cagcggactg ataaccattt ctctttatgc tttgctattg ggcttgtgac tcgttcttgg
      241 aggcaaggtt atcagattga atagaagatt cggtgatgca gcaaatagcg actgttcgtt
      301 gcggggtccg aacccgctcg gcagcaccgg gctctcccta ctatccctcg atagcagtgg
      361 cgtgcgcggg ctgtcgaccc tgtccatcct gaaagagttg atattgaagg tagtaggtac
      421 ggtgcagggt aacgcgtgat atcctgtcct cagtcttaag acttctcacc tatatcaccc
      481 gcacttaccc tagagtgccg cttaggtgct aagggcacat tgagtattgg ccgtgtagaa
      541 tatatagctt aagtacggcc aagcagacgg gaagccctgt tctccacacc ctatggtcgt
      601 atatatcagg cttctaccgg gaaacgatta agagtgtata atggactgaa aatcaatatg
      661 aacgggacaa tgctcaagtt aaattagtta ggcatcctaa tctctactaa atgttctatc
      721 tagagatcgg ggtactatag gcccgtacgt taatcactct acgcttctct cccttaggta
      781 tagtgtaggt aggggctaga catttatatg agtcagatgg tacaaacggt agcgctgcaa
      841 gaattcaagc ttggaggagt tatagacggt ccggcatagg taaggagaga ttagatattt
      901 cgacttcccg attccttagt ctccttagtc tggaaactga gaaaggcctg aactcgttca
      961 atctattagc tctaatcacg tagtctcaac tagaatcctg aacaggcgcc ggaaccagtc
     1021 caaatgcgtg gagtacagca agtccagtca aatcacgatt agaatggcca tatataagca
     1081 aaaaaccctt ggacaatacg tccgatcgac accaaagcaa tctggtacat cacccttctt
     1141 atatacaggg tagccggaat tgtattgact ctatacgaag ccgcaggttt accagcacag
     1201 aggtgcgata gctacacgaa tgtagagtga aggcattaca accgattgct aagtgtcata
     1261 gaggctaagc ttcacggctc aaggagtctg tggaggatac ctgatataag aattgagagt
     1321 tatactccgg aggttatgtt agtacctcaa aaatgtgaag tacaacgaaa ctcgaacttc
     1381 gaatgacaat agcttggctg gctgttagtc aactgaggta caacgtcatc ttgcaattag
     1441 ttatgttctt tgctagatag tgagctgctg taaacttgta gttcttagaa acatgtctga
     1501 agtctagagt cgagacttgt gattggtgta aattagtggg ggtccggagc ggaataacaa
     1561 tcgaggtcag cctcctgggt ctgtcagtaa gctaaccacg aaaggacaag cgtagacgag
     1621 gtcacggggt actggcctag ccggcttcgg ctagcgccca cgaaaatagg gcaacgtcga
     1681 agcgagagac acacacggat tccacgatcg ccgacgacaa gcactcaact ccacgacaac
     1741 ccacgacaac ccttgccgct gcgttcttgg tgaagggctg caggattaga ggtgagtatt
     1801 ttattttctg gctattgtga tctttttccc ccccggtttc cgctggttct atcttgtcga
     1861 gcgagtcggt gcgccttcga tcggtctgtc aaatttccat ccgtccactc gttcacccgg
     1921 tatcgataca agagggaatg gagcattacg acaggggagc agaggcagaa ctctcgatgt
     1981 tgctactttt tttgcttgga acaacttcct tgaaagattc ttttctgaat gcggctggga
     2041 aactgcgaat tggatatcca gcgaaaggtt accgacagac tcacggccat cgataccact
     2101 cagcgggata gaattgaagg cattcggaca ttagatgatc agcttcgctt gtttcgactc
     2161 gtgctaatca attatccctg gacagtcccc cacatagaca gcaaacaatg ccacccaagc
     2221 gcaaaacgct taacgctgcg gctgaggcta acgctcatgc cgacggacac gccgacggaa
     2281 acgccgacgg acacgtggcc aatacggccg cgtcctcgaa taatgcgagg ttcgctgatc
     2341 tcactaacat cgatactccg ggtctgggac ccacaactac gaccctgctc gtggaaccag
     2401 cacgctcaaa gcgtcaacga gtgtcccgcg catgcgacca gtgccgtgca gcccgagaga
     2461 aatgcgacgg aatacagcct gcgtgtttcc cgtgcgtttc ccagggaagg tcctgcactt
     2521 atcaggcttc gccgaaaaag aggggagttc aaaccggtta tattcgtacg ctggagctcg
     2581 ccctcgcctg gatgtttgaa aatgtcgcgc gttccgaaga tgccttgcat aacctcctcg
     2641 tccgtgacgc cggacaagga tcagctctgc tcgttggtaa agattcgccg gctgccgagc
     2701 gactccatgc ccgttgggct actagccgtg tcaataagag cattacccgc ctcctcaggt
     2761 ttcgtcagtt ggagctccct cctaccgcca cggctacggc ctcgataatg ccgcacgtga
     2821 tggagcagcc tctcagtacc agcattaacc ccgtcaacga ccgcttcaac ggtattccca
     2881 accccactcc gtataactcc gatgcagctc tcgatgctat cactcagacc aacgattatg
     2941 gaagcgtaaa tacacatggt atcctctcta cttacccgcc accggctacg caccttaatg
     3001 aagcttccgt cgctctcgct cccggtggcg cccccccccg accgcctcct ccgtatgttg
     3061 acagcacgac caatcacccg ccgtaccact cgaatctggt tccaatggcg aactttggtt
     3121 actcgaccgt tgattacgat gccatggttg acgatttggc tagcattgaa tacacggacg
     3181 ctgtggatgt cgacccacag tttatgacca atctgggatt cgttcctgga tgtaacttct
     3241 ccgacattaa tacatacgaa cagttcgtta tggtgagcaa gggcgaggag ctgttcaccg
     3301 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     3361 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     3421 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     3481 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     3541 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     3601 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     3661 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     3721 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     3781 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     3841 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     3901 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     3961 ctctcggcat ggacgagctg tacaagccaa agaagaagcg gaaggtctaa gcttacttct
     4021 ttatcggttc tctcttacga ctttttgaat ggaacgtttc cttcttctca ggcgggccta
     4081 tctttgggcc gaagctcttt tccttgtact gtaggacctg gttgataatg attcccaaaa
     4141 agacatccag catgtcagtt acttgcattc gtcagtctat acaaaagcaa tggtttagag
     4201 aaattttgaa ctttatacat ggttttattt gttgcttcac ggccgtacct tctggaaatc
     4261 cacggtagga gtgtcaattt gcgtttttga taatccttcc aaggttcttc tcgaagtagt
     4321 tgttctataa ttgcttcaca gctaccatgg aacatcccaa gcaccgctac tagaattcga
     4381 gctcggagtg gatcccccgg gctgcaggaa ttcgagctct gtacagtgac cggtgactct
     4441 ttctggcatg cggagagacg gacggacgca gagagaaggg ctgagtaata agcgccactg
     4501 cgccagacag ctctggcggc tctgaggtgc agtggatgat tattaatccg ggaccggccg
     4561 cccctccgcc ccgaagtgga aaggctggtg tgcccctcgt tgaccaagaa tctattgcat
     4621 catcggagaa tatggagctt catcgaatca ccggcagtaa gcgaaggaga atgtgaagcc
     4681 aggggtgtat agccgtcggc gaaatagcat gccattaacc taggtacaga agtccaattg
     4741 cttccgatct ggtaaaagat tcacgagata gtaccttctc cgaagtaggt agagcgagta
     4801 cccggcgcgt aagctcccta attggcccat ccggcatctg tagggcgtcc aaatatcgtg
     4861 cctctcctgc tttgcccggt gtatgaaacc ggaaaggccg ctcaggagct ggccagcggc
     4921 gcagaccggg aacacaagct ggcagtcgac ccatccggtg ctctgcactc gacctgctga
     4981 ggtccctcag tccctggtag gcagctttgc cccgtctgtc cgcccggtgt gtcggcgggg
     5041 ttgacaaggt cgttgcgtca gtccaacatt tgttgccata ttttcctgct ctccccacca
     5101 gctgctcttt tcttttctct ttcttttccc atcttcagta tattcatctt cccatccaag
     5161 aacctttatt tcccctaagt aagtactttg ctacatccat actccatcct tcccatccct
     5221 tattcctttg aacctttcag ttcgagcttt cccacttcat cgcagcttga ctaacagcta
     5281 ccccgcttga gcagacatca ccatgcctca atcctgggaa gaactggccg ctgataagcg
     5341 cgcccgcctc gcaaaaacca tccctgatga atggaaagtc cagacgctgc ctgcggatga
     5401 cagcgttatt gatttcccaa agaaatcggg gatcctttca gaggccgaac tgaagatcac
     5461 agaggcctcc gctgcagatc ttgtgtccaa gctggcggcc ggagagttga cctcggtgga
     5521 agttacgcta gcattctgta aacgggcagc aatcgcccag cagttagtag ggtcccctct
     5581 acctctcagg gagatgtaac aacgccacct tatgggacta tcaagctgac gctggcttct
     5641 gtgcagacaa actgcgccca cgagttcttc cctgacgccg ctctcgcgca ggcaagggaa
     5701 ctcgatgaat actacgcaaa gcacaagaca cccgttggtc cactccatgg cctccccatc
     5761 tctctcaaag accagcttcg agtcaaggta caccgttgcc cctaagtcgt tagatgtccc
     5821 tttttgtcag ctaacatatg ccaccagggc tacgaaacat caatgggcta catctcatgg
     5881 ctaaacaagt acgacgaagg ggactcggtt ctgacaacca tgctccgcaa agccggtgcc
     5941 gttttctacg tcaagacctc tgtcccgcag accctgatgg tctgcgagac agtcaacaac
     6001 atcatcgggc gcaccgtcaa cccacgcaac aagaactggt cgtgcggcgg cagttctggt
     6061 ggtgagggtg cgatcgttgg gattcgtggt ggcgtcatcg gtgtaggaac ggatatcggt
     6121 ggctcgattc gagtgccggc cgcgttcaac ttcctgtacg gtctaaggcc gagtcatggg
     6181 cggctgccgt atgcaaagat ggcgaacagc atggagggtc aggagacggt gcacagcgtt
     6241 gtcgggccga ttacgcactc tgttgagggt gagtccttcg cctcttcctt cttttcctgc
     6301 tctataccag gcctccactg tcctcctttc ttgcttttta tactatatac gagacgggca
     6361 gtcactgatg aagtaatgtt agacctccgc ctcttcacca aatccgtcct cggtcaggag
     6421 ccatggaaat acgactccaa ggtcatcccc atgccctggc gccagtccga gtcggacatt
     6481 attgcctcca agatcaagaa cggcgggctc aatatcggct actacaactt cgacggcaat
     6541 gtccttccac accctcctat cctgcgcggc gtggaaacca ccgtcgccgc actcgccaaa
     6601 gccggtcaca ccgtgacccc gtggacgcca tacaagcacg atttcggcca cgatctcatc
     6661 tcccatatct acgcggctga cggcagcgcc gacgtaatgc gcgatatcag tgcatccggc
     6721 gagccggcga ttccaaatat caaagaccta ctgaacccga acatcaaagc tgttaacatg
     6781 aacgagctct gggacacgca tctccagaag tggaattacc agatggagta ccttgagaaa
     6841 tggcgggagg ctgaagaaaa ggccgggaag gaactggacg ccatcatcgc gccgattacg
     6901 cctaccgctg cggtacggca tgaccagttc cggtactatg ggtatgcctc tgtgatcaac
     6961 ctgctggatt tcacgagcgt ggttgttccg gttacctttg cggataagaa catcgataag
     7021 aagaatgaga gtttcaaggc ggttagtgag cttgatgccc tcgtgcagga agagtatgat
     7081 ccggaggcgt accatggggc accggttgca gtgcaggtta tcggacggag actcagtgaa
     7141 gagaggacgt tggcgattgc agaggaagtg gggaagttgc tgggaaatgt ggtgactcca
     7201 taggcttcta ataagtgtca gatagcaatt tgcacaagaa atcaatacca gcaactgtaa
     7261 ataagcgctg aagtgaccat gccatgctac gaaagagcag aaaaaaacct gccgtagaac
     7321 cgaagagata tgacacgctt ccatctctca aaggaagaat cccttcaggg ttgcgtttcc
     7381 agtctagaca cgtataacgg cacaagtgtc tctcaccaaa tgggttatat ctcaaatgtg
     7441 atctaaggat ggaaagccca gaatattggc tgggttgatg gctgcttcga gtgcagtctc
     7501 atgctgccac aggtgactct ggatggcccc ataccactca acccatggta cgagcggtac
     7561 gctttacgaa ttcccatggg gaggggtaat cgattatcct cggataaaca cttatcccag
     7621 ggtaatcact tatccctgga taaacaatta tcctcgggta atcgcttatc cccatcaggg
     7681 aaacacgccg agcgtcctcc tccgataagc atgcgctgtc ttggtctgtc gctgtcaccg
     7741 aacatatctg tctgggcttg atttaccaat ggcctcctcc gaggacgtca tcaaggagtt
     7801 catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     7861 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     7921 cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt
     7981 gtacgtgaag caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt
     8041 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     8101 ctccctgcag gacggctcct tcatctacaa ggtgaagttc atcggcgtga acttcccctc
     8161 cgacggcccc gtaatgcaga agaaaactat gggctgggag gcctccaccg agcgcctgta
     8221 cccccgcgac ggcgtgctga agggcgagat ccacaaggcc ctgaagctga aggacggcgg
     8281 ccactacctg gtggagttca agtccatcta catggccaag aagcccgtgc agctgcccgg
     8341 ctactactac gtggactcca agctggacat cacctcccac aacgaggact acaccatcgt
     8401 ggagcagtac gagcgcgccg agggccgcca ccacctgttc ctgactcacg gtatggacga
     8461 attgtacaag tcgaagctgt aagcttgtgc ttctaaggta tgagtcgcaa aattgttttt
     8521 tatttttggt cttgagtcta atatgctcgc agctcttgcg ttgtatatgg tcgttgtcgc
     8581 gtattttctg ttgtattaaa agatcaaacg agatcaaggg atggctcgcg ggctgtctct
     8641 cgcactagga ggaagaatgc ctgaaaaagg aactttgatt ttagctgtgg aatagagatg
     8701 gcttgtttga ggacgcttgt cgcttggcgc agggacttga atggcagctt gtggaaaccg
     8761 aaggcgagaa aagtcgacgg atactgtacg tggttctatt gccagtgcgg tggaagcttc
     8821 cttctcatat agttcaatcc ttctttgaat ctgtttgttt catatttgga ctgtttcatt
     8881 ctctgcttgc gcattctcat cttcgagaac gactgcaggg attgttggtt ctgtggagct
     8941 gatgagcgcg ccttgaccac ccttgttctt gttttgctct tttgttctca tttaacccgt
     9001 ttctcccttc caaccctttg accttgcaac attgtctccc agcgcgttgc caaagcgaac
     9061 ttgatatcag tatagtatga ccaagtagtc taccaaaata aattttagta cagtattgct
     9121 agttatacaa gataaatttt gtacaacaat gattatgcag agctggtatt ctgtaaggag
     9181 taaattcatg caggtacaga gcgtccacgt caggcacacg cgctttagtg ctgggtacac
     9241 attatgctac ctttggggca aggtgctatt ctaggtaggg tatgcctagc aatgccatga
     9301 tcttatgacc tcagtaatct gaccttggat ttaagatcgg agttctcaaa ccctgcgcgc
     9361 tcagagaatt cgcatgcgga gactttagac atacctctcg ttgccgacaa atcggcattc
     9421 cgtttcggtc acggacgtaa gagttcaaca aagccgctca cctgtcccgt tcggcgcttg
     9481 agtttccact tcaaaagcac aaccgctccg atatttccat tgccaccgac caacaaaagg
     9541 ttcatatcgc gcaatagtcg tccaactctc actccgcccg cactcgatgg cgaaggatgg
     9601 aagaatatcc gagcggcatc ggataaagag atctggttct tttcgtgaag taacgtaggg
     9661 aaccgacgga aactgtagag ctataggaat ttagtatatc agaagtcaat tgagagaagt
     9721 gtatagttag taactcactt gtgccaacag gtatttccaa ccgttcttgt tcaaatttcg
     9781 tcaagtcacc atctctatgt gcaaaactgg atcatacaac agctcttatt gtagctgcat
     9841 ctcgctctca gagatcatcc tatcttcgtc gtcatccacc aacacaggtg ggagtgctag
     9901 ttgatccatg ctgaggtcaa gggacacaga cggaatgggt gtagaacgcc tcaaaacagg
     9961 taaggtatca ggatcggttg tccaggggga atccaaacca ggttccacct tgattcagag
    10021 aataagtatg acgtattctt ggcaatgttt aagcttgaga tgagccgatg tcaaactggg
    10081 agacatgatg aaggaggaaa tgttgctttg gtgggggaga cactataaat accaaagagt
    10141 gaatggttga taccatttgg ggaaggagtg tctatcgcag tctctgcaga cggcatatcg
    10201 gatagcctgc aggcgcttgt ggaggatgca catgtgaccg agggacacga agtgatccgt
    10261 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
    10321 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
    10381 gtgccagccg cctttgcgac gctcaccggg ctggttgccc tcgccgctgg gctggcggcc
    10441 gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag acaccgcggc
    10501 cgccggcgtt gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac
    10561 gttgacactt gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga
    10621 tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac
    10681 gcgagtttcc cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac
    10741 ttgaggggcg cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt
    10801 gctgacagat gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc
    10861 agcatttgca agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct
    10921 tttaaaccaa tatttataaa ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg
    10981 cgcacgccga aggggggtgc ccccccttct cgaaccctcc cggcccgcta acgcgggcct
    11041 cccatccccc caggggctgc gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc
    11101 gctggccaat tcccgagtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    11161 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    11221 gagtatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
    11281 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
    11341 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
    11401 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
    11461 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
    11521 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
    11581 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
    11641 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
    11701 ggaagaggac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
    11761 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
    11821 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
    11881 tgacattgcc ttctgcgtcc ggtcgctcag ggaggatatc ggggaagaac agtatgtcga
    11941 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
    12001 actggatgaa ttgttttagc tgtcagacca agtttactca tatatacttt agattgattt
    12061 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    12121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    12181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    12241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    12301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    12361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    12421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    12481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    12541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    12601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    12661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    12721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    12781 cgccagcaac gcggcctttt tacggttcct ggcagatcct agatgtggcg caacgatgcc
    12841 ggcgacaagc aggagcgcac cgacttcttc cgcatcaagt gttttggctc tcaggccgag
    12901 gcccacggca agtatttggg caaggggtcg ctggtattcg tgcagggcaa gattcggaat
    12961 accaagtacg agaaggacgg ccagacggtc tacgggaccg acttcattgc cgataaggtg
    13021 gattatctgg acaccaaggc accaggcggg tcaaatcagg aataagggca cattgccccg
    13081 gcgtgagtcg gggcaatccc gcaaggaggg tgaatgaatc ggacgtttga ccggaaggca
    13141 tacaggcaag aactgatcga cgcggggttt tccgccgagg atgccgaaac catcgcaagc
    13201 cgcaccgtca tgcgtgcgcc ccgcgaaacc ttccagtccg tcggctcgat ggtccagcaa
    13261 gctacggcca agatcgagcg cgacagcgtg caactggctc cccctgccct gcccgcgcca
    13321 tcggccgccg tggagcgttc gcgtcgtctt gaacaggagg cggcaggttt ggcgaagtcg
    13381 atgaccatcg acacgcgagg aactatgacg accaagaagc gaaaaaccgc cggcgaggac
    13441 ctggcaaaac aggtcagcga ggccaagcag gccgcgttgc tgaaacacac gaagcagcag
    13501 atcaaggaaa tgcagctttc cttgttcgat attgcgccgt ggccggacac gatgcgagcg
    13561 atgccaaacg acacggcccg ctctgccctg ttcaccacgc gcaacaagaa aatcccgcgc
    13621 gaggcgctgc aaaacaaggt cattttccac gtcaacaagg acgtgaagat cacctacacc
    13681 ggcgtcgagc tgcgggccga cgatgacgaa ctggtgtggc agcaggtgtt ggagtacgcg
    13741 aagcgcaccc ctatcggcga gccgatcacc ttcacgttct acgagctttg ccaggacctg
    13801 ggctggtcga tcaatggccg gtattacacg aaggccgagg aatgcctgtc gcgcctacag
    13861 gcgacggcga tgggcttcac gtccgaccgc gttgggcacc tggaatcggt gtcgctgctg
    13921 caccgcttcc gcgtcctgga ccgtggcaag aaaacgtccc gttgccaggt cctgatcgac
    13981 gaggaaatcg tcgtgctgtt tgctggcgac cactacacga aattcatatg ggagaagtac
    14041 cgcaagctgt cgccgacggc ccgacggatg ttcgactatt tcagctcgca ccgggagccg
    14101 tacccgctca agctggaaac cttccgcctc atgtgcggat cggattccac ccgcgtgaag
    14161 aagtggcgcg agcaggtcgg cgaagcctgc gaagagttgc gaggcagcgg cctggtggaa
    14221 cacgcctggg tcaatgatga cctggtgcat tgcaaacgct agggccttgt ggggtcagtt
    14281 ccggctgggg gttcagcagc ccctgctcgg atctgttgga ccggacagta gtcatggttg
    14341 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
    14401 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca
    14461 cattgcggac gtttttaatg tactggggtt gaacactct
//
